PDF
Full
Outline
Objective To breed Bifidobacterium adolescentis strains that can adapt to the gastrointestinal environment of felines and have strong intestinal colonization capabilities. Methods B. adolescentis was isolated from the feces of felines with long and regular life spans. The original strain QC-Y (with the life span extension rate reaching 33.85%) was selected through biomass assessments and mouse life span experiments and it was identified as B. adolescentis. After radiation-induced mutation, QC-Y-09 was screened out by the biomass assessment, gastrointestinal tolerance domestication, and evaluation. Results QC-Y-09 showed the biomass 55.667 times and the tolerance score 5.66 times that of QC-Y. Moreover, the tolerance of the strain to the feline gastrointestinal environment showed good genetic stability. The survival rates of the 10th generation of QC-Y-09 in the artificial gastric juice, intestinal juice, anaerobic, and micro-aerobic environments were 18.80%, 41.60%, 93.26%, and 48.39%, respectively, which were 62.67%, 108.53%, 97.92%, and 94.40% of those of the original generation. The intestinal colonization test showed that the colonization ability of QC-Y-09 in felines was significantly stronger than that of QC-Y and human-derived B. adolescentis. Seven days after the feeding of the microbial inoculum was stopped, the viable count of B. adolescentis in the feline feces of the QC-Y-09 group still reached 4.37 lg CFU/g, which was 1.74 lg CFU/g and 3.02 lg CFU/g, respectively, higher than those of the QC-Y group and the human-derived B. adolescentis group. In addition, QC-Y-09 had a good relieving effect on the feline food change stress, reducing the feline food change stress rate by 85.71%. The results of SNP analysis showed that QC-Y-09 was significantly different from QC-Y at the gene level, and the differentially expressed genes were mainly enriched in the ribosome structure and aminoacyl-tRNA biosynthesis pathway. Conclusion Feline-derived B. adolescentis QC-Y-09 bred in this study can effectively adapt to the gastrointestinal environment and colonize the intestines of felines. This study provides both theoretical and practical bases for the application of B. adolescentis in functional food for felines.
PDF
277
111
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
