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Isolation, characterization, and degradation pathway prediction of a myosmine-degrading bacterial strain Shinella G-2
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Hui GAO1, Mengmeng YU1, Ruyun ZHANG1, Wei JIA1, Xi ZHANG2, Bo FU1, Zicheng XU1, Zhigang GUO2, *, Bingjun DANG1, *
Acta Microbiologica Sinica | 2025, 65(11) : 5074 - 5091
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Acta Microbiologica Sinica | 2025, 65(11): 5074-5091
• Research Article •
Isolation, characterization, and degradation pathway prediction of a myosmine-degrading bacterial strain Shinella G-2
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Hui GAO1, Mengmeng YU1, Ruyun ZHANG1, Wei JIA1, Xi ZHANG2, Bo FU1, Zicheng XU1, Zhigang GUO2, *, Bingjun DANG1, *
Affiliations
  • 1 College of Tobacco Science, Henan Agricultural University, Zhengzhou, Henan, China
  • 2 China Tobacco Shaanxi Industrial Co. , Ltd. , Xi’an, Shaanxi, China
Published: 2025-11-04 doi: 10.13343/j.cnki.wsxb.20250304
Outline
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Myosmine, also known as 3-(3,4-dihydro-2H-pyrrol-5-yl) pyridine, is a tobacco alkaloid found not only in tobacco but also in various foods, fruits, and vegetables. It serves as one of the precursors for the formation of the carcinogenic tobacco-specific nitrosamine N′-nitrosonornicotine, posing a potential threat to human health. Objective To screen bacterial strains capable of degrading myosmine and preliminarily explore the pathways and mechanisms underlying myosmine degradation. Methods We used myosmine as the sole carbon source to enrich and isolate the myosmine-degrading bacterial strain from tobacco-growing soil. Taxonomic identification of this myosmine-degrading strain was achieved by a combination of morphological observation, physiological and biochemical testing, and molecular analysis. The myosmine degradation products by this strain were analyzed by HPLC and UHPLC-MS/MS. The degradation genes were predicted by BLAST comparison. Results A strain G-2 capable of degrading myosmine was successfully isolated. The strain was identified as a member of Shinella, designated Shinella sp. G-2. HPLC and UHPLC-MS/MS identified five degradation products. Genomic analysis showed that strain G-2 possessed a homologous gene cluster of a variant of the pyridine and pyrrolidine pathway (VPP) gene cluster. Conclusion In this study, a strain Shinella sp. G-2 with the ability to degrade myosmine was isolated. Strain G-2 might use enzymes in the VPP pathway to degrade myosmine through a metabolic pathway similar to the VPP pathway.

Shinella sp. G-2  /  myosmine  /  degradation products  /  degradation pathway
Hui GAO, Mengmeng YU, Ruyun ZHANG, Wei JIA, Xi ZHANG, Bo FU, Zicheng XU, Zhigang GUO, Bingjun DANG. Isolation, characterization, and degradation pathway prediction of a myosmine-degrading bacterial strain Shinella G-2[J]. Acta Microbiologica Sinica, 2025 , 65 (11) : 5074 -5091 . DOI: 10.13343/j.cnki.wsxb.20250304
Funding
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  • Science and Technology Project of China Tobacco Shaanxi Industrial Co., Ltd(BA000-ZB23007)
  • Young Talents Foundation of Henan Agricultural University(30500568)
Appendix
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Year 2025 volume 65 Issue 11
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252
106
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Article Info
doi: 10.13343/j.cnki.wsxb.20250304
  • Receive Date：2025-04-12
  • Online Date：2025-11-10
  • Published：2025-11-04
Article Data
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History
  • Received：2025-04-12
  • Accepted：2025-07-10
Funding
Science and Technology Project of China Tobacco Shaanxi Industrial Co., Ltd(BA000-ZB23007)
Young Talents Foundation of Henan Agricultural University(30500568)
Affiliations
    1 College of Tobacco Science, Henan Agricultural University, Zhengzhou, Henan, China
    2 China Tobacco Shaanxi Industrial Co. , Ltd. , Xi’an, Shaanxi, China

Corresponding:

*E-mail: GUO Zhigang,
DANG Bingjun,
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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