PDF
Full
Outline
Objective To elucidate and compare the diversity, structure, and functional characteristics of gut microbiota in sympatric fish species from a perspective of microbial ecology and explore the role of gut microbiota in feeding habit and ecological niche differentiation. Methods Foregut, midgut, and hindgut samples from Gymnocypris eckloni and Schizopygopsis pylzovi, along with their aquatic environmental samples, were collected from the upper Yellow River. The gut microbiota and potential functions were compared by 16S rRNA gene high-throughput sequencing and multiple bioinformatics approaches. Results Microbial alpha diversity followed the trend of aquatic environment>S. pylzovi>G. eckloni (P<0.05). In G. eckloni, alpha diversity was highest in the foregut, whereas in S. pylzovi, it decreased progressively from the foregut to the midgut and then to the hindgut. Beta diversity analysis based on clustering and CPCoA demonstrated that microbial communities derived from different gut segments of the same species were more similar to each other than those derived from the same gut segment of different species, indicating that the differences attributable to species were greater than those attributable to gut segments (P<0.001). Pseudomonadota accounted for over 50.00% of the gut microbiota in all sample types, while Bacillota comprised more than 25.00% in the guts of both fish species, which was significantly higher than that (3.80%) observed in the aquatic environment. Fusobacteriota and Cetobacterium were nearly absent in water and S. pylzovi but showed high abundance in the midgut and hindgut of G. eckloni. Enterococcus was specifically enriched in the hindgut of G. eckloni, while Lactococcus were predominantly found in the hindgut of S. pylzovi. PICRUS2 functional prediction revealed that gut microbiota of both species primarily enriched amino acid and carbohydrate metabolism pathways. The enriched metabolic pathways varied significantly across different gut segments of S. pylzovi, while significant differences in signaling molecules and interaction, cardiovascular diseases, and metabolism of terpenoids and polyketides were noted for the same gut segments between the two species. Conclusion There were significant differences in the microbiota composition and diversity in the gut between the two fish species and their aquatic environments, with distinct gut microbiota functions for each species. This study establishes a micrological foundation for research on the feeding habit and ecological niche differentiation of fish and provides theoretical support for exploitation of gut microbial resources and conservation and resource management of plateau fish species.
PDF
197
70
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
