Home Journals Articles Updates About
CN
image
收藏切换
Pathogenicity and fungicide resistance of Lichtheimiaramosa LYSF001: analysis of virulence genes and evaluation of sensitivity to fungicides
收藏切换
PDF
Keyu ZOU1, Yanwen YANG1, Liu XING1, Jingyun CHEN1, Li WANG1, Yong WEI2
Acta Microbiologica Sinica | 2025, 65(10) : 4517 - 4527
Less
收藏切换
Acta Microbiologica Sinica | 2025, 65(10): 4517-4527
• Research Article •
Pathogenicity and fungicide resistance of Lichtheimiaramosa LYSF001: analysis of virulence genes and evaluation of sensitivity to fungicides
Full
Keyu ZOU1, Yanwen YANG1, Liu XING1, Jingyun CHEN1, Li WANG1, Yong WEI2
Affiliations
  • 1Key Laboratory of Qinghai-Xizang Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, Sichuan, China
  • 2Sichuan Animal Science Academy, Chengdu, Sichuan, China
Published: 2025-09-04 doi: 10.13343/j.cnki.wsxb.20250205
Outline
收藏切换

[Objective] To investigate the pathogenicity of Lichtheimiaramosa LYSF001 and ITS resistance to common fungicides, providing experimental evidence for the diagnosis and treatment of fungal infections in animals. [Methods] The strain was identified by morphological characterization and molecular biology techniques. PCR was employed to detect virulence genes in strain LYSF001, and an animal infection model was established to evaluate the pathogenicity of the strain. Histopathological examination, Grocott’s methenamine silver (GMS) staining, and ITS sequences amplification were employed to analyze pathological changes in the livers and kidneys of infected animals. Additionally, antimicrobial susceptibility tests were conducted to assess the sensitivity of strain LYSF001 to caspofungin, amphotericin B, and itraconazole. [Results] Strain LYSF001 was identified as L. ramosa. PCR analysis revealed that strain LYSF001 carried five virulence genes (CalA, PKP2, LaeA, Alp2, and AspF1). Animal experiments demonstrated that the strain led to the mortality of 40% and caused visceral hyperemia in mice, with the most significant pathological changes (inflammatory cell infiltration and tissue necrosis) observed in the livers and kidneys. Severe infections led to animal mortality. GMS staining revealed the presence of dark-colored hyphae in the heart and liver, and ITS sequences amplification further confirmed fungal infection. The antimicrobial susceptibility test results indicated that strain LYSF001 was resistant to caspofungin but sensitive to amphotericin B and itraconazole. [Conclusion] L. ramosa LYSF001 exhibits strong pathogenicity, capable of causing severe infections and even death in animals. Additionally, the strain showed resistance to caspofungin but sensitivity to amphotericin B and itraconazole. The findings provide important experimental evidence and technical support for the diagnosis and treatment of fungal infections in animals.

Lichtheimiaramosa  /  virulence genes  /  pathogenicity  /  fungicide resistance
Keyu ZOU, Yanwen YANG, Liu XING, Jingyun CHEN, Li WANG, Yong WEI. Pathogenicity and fungicide resistance of Lichtheimiaramosa LYSF001: analysis of virulence genes and evaluation of sensitivity to fungicides[J]. Acta Microbiologica Sinica, 2025 , 65 (10) : 4517 -4527 . DOI: 10.13343/j.cnki.wsxb.20250205
Funding
Less
  • the Sichuan Meat Sheep Innovation Team—Epidemic Prevention Position(sccxtd-2024-14)
  • the Southwest Minzu University for the Fundamental Research Funds for the Central Universities(ZYN2025149)
Appendix
Less
Year 2025 volume 65 Issue 10
PDF
261
93
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20250205
  • Receive Date：2025-03-17
  • Online Date：2025-11-03
  • Published：2025-09-04
Article Data
Affiliations
History
  • Received：2025-03-17
  • Accepted：2025-05-06
Funding
the Sichuan Meat Sheep Innovation Team—Epidemic Prevention Position(sccxtd-2024-14)
the Southwest Minzu University for the Fundamental Research Funds for the Central Universities(ZYN2025149)
Affiliations
    1Key Laboratory of Qinghai-Xizang Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu, Sichuan, China
    2Sichuan Animal Science Academy, Chengdu, Sichuan, China

Corresponding:

E-mail: WANG Li,
WEI Yong,
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20250205
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House