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An lpxtg270 knockoutstrainof Corynebacterium pseudotuberculosis: construction, biological characterization, and evaluation of pathogenicity in mice
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Luting NIU1, Hong LYU1, Lianghui LONG1, Yiqian ZHANG1, Chengwei XU1, Xinzhi ZHOU1, Xiaohua WANG2, Zhiying WANG1, Zuoyong ZHOU1
Acta Microbiologica Sinica | 2025, 65(10) : 4550 - 4564
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Acta Microbiologica Sinica | 2025, 65(10): 4550-4564
• Research Article •
An lpxtg270 knockoutstrainof Corynebacterium pseudotuberculosis: construction, biological characterization, and evaluation of pathogenicity in mice
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Luting NIU1, Hong LYU1, Lianghui LONG1, Yiqian ZHANG1, Chengwei XU1, Xinzhi ZHOU1, Xiaohua WANG2, Zhiying WANG1, Zuoyong ZHOU1
Affiliations
  • 1College of Veterinary Medicine, Southwest University, Chongqing, China
  • 2Jinghua Pharmaceutical (Chengdu) Co. , Ltd. , Chengdu, Sichuan, China
Published: 2025-09-04 doi: 10.13343/j.cnki.wsxb.20250217
Outline
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[Objective] To construct a strain XH02Δlpxtg270 with knockout of the LPXTG motif protein-coding gene from Corynebacterium pseudotuberculosis XH02 and explore the role of lpxtg270 in the growth, biofilm formation, and infection of XH02. [Methods] CRISPR/Cas9 was employed to construct XH02Δlpxtg270. The knockout strain and the wild strain XH02 were compared in terms of biological characteristics, invasion into J774A.1 macrophages, and pathogenicity in mice. [Results] Compared with XH02, XH02Δlpxtg270 did not change significantly in the colony morphology, growth curve, adhesion to J774A.1 macrophages, or intracellular proliferation, while it demonstrated reductions in the biofilm formation and invasion into J774 A.1 cells. Moreover, the release of lactate dehydrogenase and secretion of interleukin-1β from J774 A.1 cells infected with the knockout strain decreased compared with those infected with the wild strain. Compared with XH02, XH02Δlpxtg270 showed weakened pathogenicity in mice and decreased loads in the liver, spleen, kidney, lung, and brain, causing milder pathological changes of above organs in mice. [Conclusion] LPXTG270 of C. pseudotuberculosis is related to the biofilm formation and invasion into macrophages, playing a key role in the pathogenicity of this bacterium in mice.

Corynebacterium pseudotuberculosis  /  LPXTG motif protein-coding gene lpxtg270  /  gene knockout  /  biological characteristic  /  pathogenicity
Luting NIU, Hong LYU, Lianghui LONG, Yiqian ZHANG, Chengwei XU, Xinzhi ZHOU, Xiaohua WANG, Zhiying WANG, Zuoyong ZHOU. An lpxtg270 knockoutstrainof Corynebacterium pseudotuberculosis: construction, biological characterization, and evaluation of pathogenicity in mice[J]. Acta Microbiologica Sinica, 2025 , 65 (10) : 4550 -4564 . DOI: 10.13343/j.cnki.wsxb.20250217
Funding
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  • the National College Students Innovation Training Program(202410635070)
  • the Chongqing Natural Science Foundation(CSTB2024NSCQ-MSX1262)
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Year 2025 volume 65 Issue 10
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Article Info
doi: 10.13343/j.cnki.wsxb.20250217
  • Receive Date：2025-03-19
  • Online Date：2025-11-03
  • Published：2025-09-04
Article Data
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History
  • Received：2025-03-19
  • Accepted：2025-05-09
Funding
the National College Students Innovation Training Program(202410635070)
the Chongqing Natural Science Foundation(CSTB2024NSCQ-MSX1262)
Affiliations
    1College of Veterinary Medicine, Southwest University, Chongqing, China
    2Jinghua Pharmaceutical (Chengdu) Co. , Ltd. , Chengdu, Sichuan, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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