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Single-chain dimerization enhances the immunogenicity of rotavirus ∆VP8* vaccine
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Chunyu WANG1, 2, Zhiyu SONG2, Yan HUO2, Han LI3, Huiying JI1, Tianhao ZHANG2, Zhimin LIU2, 3, Rongxiang FANG2, Lili ZHANG2
Acta Microbiologica Sinica | 2025, 65(10) : 4392 - 4405
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Acta Microbiologica Sinica | 2025, 65(10): 4392-4405
• Research Article •
Single-chain dimerization enhances the immunogenicity of rotavirus ∆VP8* vaccine
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Chunyu WANG1, 2, Zhiyu SONG2, Yan HUO2, Han LI3, Huiying JI1, Tianhao ZHANG2, Zhimin LIU2, 3, Rongxiang FANG2, Lili ZHANG2
Affiliations
  • 1 School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, China
  • 2 State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
  • 3 College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, Shanxi, China
Published: 2025-09-04 doi: 10.13343/j.cnki.wsxb.20250133
Outline
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[Objective] Rotavirus (RV) is a major pathogen causing acute dehydrating gastroenteritis in infants and young children. Currently, no specific therapeutic drugs are available, making preventive vaccination the most effective strategy for controlling RV infection. We targeted the RV receptor-binding domain viral protein 8* (VP8*) and selected its functional region ΔVP8* (amino acids 65-223) to construct a single-chain dimer ΔVP8*-sc-dimer. We expressed and purified this recombinant protein in a prokaryotic system using the pET-30a(+) vector and evaluated its immunogenicity and neutralizing antibody induction capacity to provide scientific evidence for developing safe and effective RV subunit vaccines. [Methods] The ΔVP8*-sc-dimer sequence was synthesized and cloned into the prokaryotic expression vector pET-30a(+) via homologous recombination. The purified recombinant protein was formulated with AddaVax adjuvant and administered to 6 to 7-week-old BALB/c mice via intramuscular injection. ΔVP8*-specific IgG antibody titers in sera were determined by enzyme-linked immunosorbent assay (ELISA), and neutralization activity of immune sera was assessed through virus neutralization assays. [Results] The recombinant protein ΔVP8*-sc-dimer was successfully expressed with 90% purity. ELISA results showed that both ΔVP8* and ΔVP8*-sc-dimer induced specific anti-ΔVP8* IgG antibodies following immunization, with the ΔVP8*-sc-dimer group exhibiting significantly higher antibody titers. Virus neutralization assays revealed that immune sera from both groups neutralized the RV Wa strain, with the ΔVP8*-sc-dimer group showing significantly superior neutralizing antibody titers. [Conclusion] The ΔVP8*-sc-dimer subunit vaccine effectively stimulates high-level antibody production against RV Wa strain, demonstrating significantly enhanced immune responses compared with ΔVP8*. With its excellent immunogenicity, ΔVP8*-sc-dimer represents a promising candidate antigen for developing novel RV vaccines with substantial clinical application potential.

human rotavirus  /  subunit vaccine  /  recombinant protein  /  ΔVP8* homodimer  /  immunogenicity
Chunyu WANG, Zhiyu SONG, Yan HUO, Han LI, Huiying JI, Tianhao ZHANG, Zhimin LIU, Rongxiang FANG, Lili ZHANG. Single-chain dimerization enhances the immunogenicity of rotavirus ∆VP8* vaccine[J]. Acta Microbiologica Sinica, 2025 , 65 (10) : 4392 -4405 . DOI: 10.13343/j.cnki.wsxb.20250133
Funding
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  • the Contract Research Project Quanzhou Runyuan Medical Technology Co., Ltd(2025110043103983)
  • the Key Deployment Project Support Fund of the “Three-year Action Plan” of the Institute of Microbiology, Chinese Academy of Sciences(E2SJ060603)
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Year 2025 volume 65 Issue 10
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Article Info
doi: 10.13343/j.cnki.wsxb.20250133
  • Receive Date：2025-02-24
  • Online Date：2025-11-03
  • Published：2025-09-04
Article Data
Affiliations
History
  • Received：2025-02-24
  • Accepted：2025-06-23
Funding
the Contract Research Project Quanzhou Runyuan Medical Technology Co., Ltd(2025110043103983)
the Key Deployment Project Support Fund of the “Three-year Action Plan” of the Institute of Microbiology, Chinese Academy of Sciences(E2SJ060603)
Affiliations
    1 School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, China
    2 State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
    3 College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, Shanxi, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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