Home Journals Articles Updates About
CN
image
收藏切换
Biological characteristics of the transposon insertion mutant of TonB in Avibacterium paragallinarum
收藏切换
PDF
Bingbing FAN1, 2, Yan SHAO2, Quan ZHANG2, Lulu LI2, Jingwen LU3, Shuqi LU2, Weiwei XU2, Weidong SUN1, Wei JIANG2
Acta Microbiologica Sinica | 2025, 65(10) : 4528 - 4536
Less
收藏切换
Acta Microbiologica Sinica | 2025, 65(10): 4528-4536
• Research Article •
Biological characteristics of the transposon insertion mutant of TonB in Avibacterium paragallinarum
Full
Bingbing FAN1, 2, Yan SHAO2, Quan ZHANG2, Lulu LI2, Jingwen LU3, Shuqi LU2, Weiwei XU2, Weidong SUN1, Wei JIANG2
Affiliations
  • 1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China
  • 2Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China
  • 3Shanghai University of Medicine & Health Sciences, Shanghai, China
Published: 2025-09-04 doi: 10.13343/j.cnki.wsxb.20250214
Outline
收藏切换

[Objective] To elucidate the virulence-associated biological characteristics of a transposon insertion mutant with impaired biofilm formation of Avibacterium paragallinarum. [Methods] The wild-type strain JZIC-005 and a biofilm-deficient mutant Tn-1504 (from a pre-established transposon mutant library) were selected for determination of insertion sites biological characterization. [Results] PCR results demonstrated site-specific transposon integration into TonB of A. paragallinarum. While mutant Tn-1504 exhibited no growth defects compared with the wild-type strain (P>0.05), it demonstrated reductions of 46.57% in adhesion to DF-1 cells and 77.61% in cellular invasion. Cytotoxicity assays revealed a 34.97% reduction of Tn-1504 in inducing host cell damage. All of the above phenotypic differences were statistically significant (P<0.01). [Conclusion] We demonstrate that inactivation of TonB specifically impairs the adhesion, invasion, and virulence characteristics of A. paragallinarum, providing a key target for analyzing the pathogenic mechanism and developing anti-virulence strategies for this pathogen.

Avibacterium paragallinarum  /  transposon  /  biological characteristics  /  TonB
Bingbing FAN, Yan SHAO, Quan ZHANG, Lulu LI, Jingwen LU, Shuqi LU, Weiwei XU, Weidong SUN, Wei JIANG. Biological characteristics of the transposon insertion mutant of TonB in Avibacterium paragallinarum[J]. Acta Microbiologica Sinica, 2025 , 65 (10) : 4528 -4536 . DOI: 10.13343/j.cnki.wsxb.20250214
Funding
Less
  • the National Key Research and Development Program of China(2023YFD1800602)
  • the National Natural Science Foundation of China(32473039)
Appendix
Less
Year 2025 volume 65 Issue 10
PDF
211
72
Cite this Article
BibTeX
Article Info
doi: 10.13343/j.cnki.wsxb.20250214
  • Receive Date：2025-03-18
  • Online Date：2025-11-03
  • Published：2025-09-04
Article Data
Affiliations
History
  • Received：2025-03-18
  • Accepted：2025-04-10
Funding
the National Key Research and Development Program of China(2023YFD1800602)
the National Natural Science Foundation of China(32473039)
Affiliations
    1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China
    2Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China
    3Shanghai University of Medicine & Health Sciences, Shanghai, China

Corresponding:

E-mail: JIANG Wei,
SUN Weidong,
References
Share
https://castjournals.cast.org.cn/joweb/wswxb/EN/10.13343/j.cnki.wsxb.20250214
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House