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Overexpression of the phosphatase gene Ssppe1 regulates the sexual mating and pathogenicity of Sporisorium scitamineum
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Jinfeng QIU1, 3, 4, Zhuyi HU2, Ailing ZHOU2, Haoming WU1, 3, 4, Lijiu ZHAO1, 2, 4, Ru LI1, 2, 3
Acta Microbiologica Sinica | 2026, 66(7) : 3526 - 3543
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Acta Microbiologica Sinica | 2026, 66(7): 3526-3543
• Research Article •
Overexpression of the phosphatase gene Ssppe1 regulates the sexual mating and pathogenicity of Sporisorium scitamineum
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Jinfeng QIU1, 3, 4, Zhuyi HU2, Ailing ZHOU2, Haoming WU1, 3, 4, Lijiu ZHAO1, 2, 4, Ru LI1, 2, 3
Affiliations
  • 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Nanning, Guangxi, China
  • 2.College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China
  • 3.Guangxi Key Laboratory of Sugarcane Biology, Nanning, Guangxi, China
  • 4.College of Agriculture, Guangxi University, Nanning, Guangxi, China
Published: 2026-07-04 doi: 10.13343/j.cnki.wsxb.20250970
Outline
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Sugarcane smut is a severe fungal disease caused by Sporisorium scitamineum, resulting in yield reduction and economic losses. Reversible protein phosphorylation plays a crucial role in the sexual mating and pathogenicity of S. scitamineum. Protein phosphatases, as key regulators of reversible protein phosphorylation, remain poorly characterized in S. scitamineum. Objective To elucidate the biological functions of the protein phosphatase SsPpe1 in S. scitamineum, providing a potential target for effective control of sugarcane smut. Methods We constructed overexpression mutants OE-Ssppe1 by Agrobacterium-mediated genetic transformation technology and analyzed the sporidium morphology, sexual mating ability, stress tolerance, and pathogenicity. Results The OE-Ssppe1 sporidia exhibited pseudohyphal morphology with multiple nuclei and abnormal chitin accumulation. The OE-Ssppe1 mutants showed reduced tolerance to NaCl and SDS, sexual mating, and pathogenicity. RT-qPCR and RNA-seq analyses revealed that Ssppe1 overexpression affected the expression of genes related to pheromone response, MAPK, and cAMP-PKA signaling pathways. In addition, Ssppe1 overexpression affected protein synthesis and folding process. Conclusion The protein phosphatase SsPpe1 is involved in regulating the sporidium morphology, stress responses, sexual mating, and pathogenicity of S. scitamineum. These findings provide a theoretical basis for thoroughly elucidating the pathogenic mechanisms of S. scitamineum and developing targeted disease control strategies.

Sporisorium scitamineum  /  protein phosphatase  /  SsPpe1  /  sexual mating  /  pathogenicity
Jinfeng QIU, Zhuyi HU, Ailing ZHOU, Haoming WU, Lijiu ZHAO, Ru LI. Overexpression of the phosphatase gene Ssppe1 regulates the sexual mating and pathogenicity of Sporisorium scitamineum[J]. Acta Microbiologica Sinica, 2026 , 66 (7) : 3526 -3543 . DOI: 10.13343/j.cnki.wsxb.20250970
Funding
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  • The National Natural Science Foundation of China(32472510)
  • The Guangxi Zhuang Autonomous Region Science and Technology Major Program(GKAA24206010-1)
Appendix
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Year 2026 volume 66 Issue 7
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113
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Article Info
doi: 10.13343/j.cnki.wsxb.20250970
  • Receive Date：2025-12-25
  • Online Date：2026-07-06
  • Published：2026-07-04
Article Data
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History
  • Received：2025-12-25
  • Accepted：2026-02-12
Funding
The National Natural Science Foundation of China(32472510)
The Guangxi Zhuang Autonomous Region Science and Technology Major Program(GKAA24206010-1)
Affiliations
    1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Nanning, Guangxi, China
    2.College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China
    3.Guangxi Key Laboratory of Sugarcane Biology, Nanning, Guangxi, China
    4.College of Agriculture, Guangxi University, Nanning, Guangxi, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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