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Objective To address the limitations of current hair-loss treatments, we employed an in vitro human dermal papilla cell (HDPC) model and a telogen C57BL/6 mouse model to study the effects of Lactiplantibacillus plantarum CCFM1352 fermentation metabolites and bacterial preparations on the hair follicle cycle and decipher the associated molecular mechanisms. Methods HDPCs were treated with different concentrations of CCFM1352 fermentation metabolites or bacterial lysates in vitro, and cell viability as well as the expression of molecules related to Wnt/β-catenin signaling, anagen/catagen regulation, and apoptosis was assessed. Telogen C57BL/6 mice were administrated with the fermentation metabolites or heat-killed bacterial cells by gavage for 21 days, and changes in hair coverage, follicular structure, and associated signaling molecules were analyzed. Results CCFM1352 fermentation metabolites at a volume fraction of 10% increased the viability of HDPCs to 135.30%, up-regulated Wnt10b and FGF-7, down-regulated DKK1 and TGF-β1, and increased Bcl-2 and decreased Bax, inducing a favorable change pattern associated with the anagen phase. Continuous gavage of the fermentation metabolites promoted the transition of hair follicles from telogen to anagen, increased the hair coverage to 61.62%, and enhanced hair follicle density, dermal thickness, and β-catenin accumulation and nuclear translocation. However, the regulatory effects of bacterial lysate and heat-killed bacteria were weaker in both models and showed only limited improvements in some indices. Conclusion CCFM1352 fermentation metabolites could modulate Wnt/β-catenin-related molecules and the expression of key factors such as FGF-7, TGF-β1, Bcl-2, and Bax, which favors the transition of hair follicles from telogen to anagen and suggests an important role in promoting hair growth. This study provides experimental evidence for the application of bioactive metabolites from probiotics in the field of hair health.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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