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Objective To clarify the role and molecular mechanism of the host protein solute carrier family 25 member 6 (SLC25A6) during fowl adenovirus serotype-4 (FAdV-4) infection, thus providing a theoretical basis for elucidating the pathogenic mechanism of FAdV-4 and developing novel prevention and control strategies. Methods First, we confirmed that infection of LMH cells with FAdV-4 at different time points and multiplicities of infection (MOI) resulted in obvious cytopathic effects (CPE). Second, on the basis of the host protein SLC25A6 identified in previous screening, Western blotting was employed to examine the effect of FAdV-4 on the expression of endogenous SLC25A6 in cells. Subsequently, transfection experiments were performed to regulate the expression of SLC25A6 (overexpression or interference with endogenous expression). RT-qPCR and Western blotting were employed to analyze the effect of SLC25A6 on FAdV-4 replication from the aspects of mRNA level, protein level, and viral titer. Finally, co-immunoprecipitation (Co-IP) was employed to verify the interaction between SLC25A6 and the core capsid protein Hexon of FAdV-4. Results FAdV-4 significantly inhibited the expression of endogenous SLC25A6 in cells. The overexpression of SLC25A6 markedly inhibited FAdV-4 replication, while interference with endogenous SLC25A6 promoted viral replication. SLC25A6 could directly interact with the Hexon protein of FAdV-4. Conclusion The host protein SLC25A6 inhibits FAdV-4 replication through its interaction with the viral Hexon protein. The results provide a theoretical basis for further elucidating the pathogenic mechanism of FAdV-4 and developing novel prevention and control strategies.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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