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Interaction between type Ⅰ signal peptidase and signal peptides in Bacillus amyloliquefaciens
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Jiejie GUO, Weihong WANG, Jiemin HE, Dengke LI, Yexue LIU, Fuping LU, Yu LI*
Acta Microbiologica Sinica | 2025, 65(1) : 256 - 267
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Acta Microbiologica Sinica | 2025, 65(1): 256-267
• Research Articles •
Interaction between type Ⅰ signal peptidase and signal peptides in Bacillus amyloliquefaciens
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Jiejie GUO, Weihong WANG, Jiemin HE, Dengke LI, Yexue LIU, Fuping LU, Yu LI*
Affiliations
  • Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China
Published: 2025-01-04 doi: 10.13343/j.cnki.wsxb.20240504
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[Objective] Based on the critical role of type Ⅰ signal peptidase in the secretion system, this study explores the interaction between signal peptidase and signal peptides to guide the optimization of aminopeptidase secretion expression in Bacillus amyloliquefaciens. [Methods] The endogenous signal peptidase and signal peptide of Bacillus amyloliquefaciens TCCC 19030 were examined using relative fluorescence intensity and enzyme activity for analysis, and molecular docking to study their interaction. [Results] The signal peptide YolC fused with aminopeptidase exhibited the highest extracellular enzyme activity, reaching 11 847.67 U/mL. Overexpression of the signal peptidase SipW increased aminopeptidase activity to 16 261 U/mL. Molecular docking results also showed that YolC had the lowest binding free energy with SipW, at −4.4 kcal/mol. [Conclusion] Optimization of signal peptides and overexpression of signal peptidase can effectively enhance the secretion of aminopeptidase. The binding energy between signal peptidase and signal peptide is a key factor influencing the secretion levels of the target protein.

signal peptidase  /  signal peptide  /  molecular docking
郭洁洁, 王伟红, 何杰民, 李登科, 刘业学, 路福平, 李玉. 解淀粉芽孢杆菌Ⅰ型信号肽酶与信号肽的相互作用. 微生物学报, 2025 , 65 (1) : 256 -267 . DOI: 10.13343/j.cnki.wsxb.20240504
Jiejie GUO, Weihong WANG, Jiemin HE, Dengke LI, Yexue LIU, Fuping LU, Yu LI. Interaction between type Ⅰ signal peptidase and signal peptides in Bacillus amyloliquefaciens[J]. Acta Microbiologica Sinica, 2025 , 65 (1) : 256 -267 . DOI: 10.13343/j.cnki.wsxb.20240504
Funding
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  • National Key Research and Development Program of China(2021YFC2104000)
Appendix
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Year 2025 volume 65 Issue 1
841
411
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Article Info
doi: 10.13343/j.cnki.wsxb.20240504
  • Receive Date：2024-08-15
  • Online Date：2026-03-21
  • Published：2025-01-04
Article Data
Affiliations
History
  • Received：2024-08-15
  • Accepted：2024-10-30
Funding
National Key Research and Development Program of China(2021YFC2104000)
Affiliations
    Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China

Corresponding:

*LI Yu E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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