In view of the problems existing in the copper and sulfur separation process of a certain concentrator, such as high copper grade (>0.19%), large circulation load and low copper-sulfur separation efficiency of the tailings, operation process of one-roughing, three-scavenging and two-cleaning for cleaning stage of copper-sulfur separation was formed. Investigation of current status of the on-site process flow and mineralogy analysis of intermediate products in cleaning stage were conducted. On-site process optimization industrial tests were executed based on small-scale experiments on intermediate product grinding. Industrial test results of process flow transformation showed that the copper grade of tailings in cleaning stage dropped from 0.19% to 0.156%; the copper recovery rate in cleaning stage increased from 98.04% to 98.56%. The proposed process technological transformation can create economic benefits of about 16 million yuan per year.