Article(id=1261728988259561985, tenantId=1146029695717560320, journalId=1146119944283992078, issueId=1261728796072358112, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1778749232592, onlineDateStr=2026-05-14, pubDate=1774195200000, pubDateStr=2026-03-23, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1778749232592, onlineIssueDateStr=2026-05-14, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1778749232592, creator=13701087609, updateTime=1778749232592, updator=13701087609, issue=Issue{id=1261728796072358112, tenantId=1146029695717560320, journalId=1146119944283992078, year='2026', volume='4', issue='6', pageStart='1', pageEnd='234', issueExtLink='null', onlineDate='null', pubDate='1774195200000', pubDateStr='2026-03-23', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1778749186771, creator='13701087609', updateTime=1778750183088, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1261732975067148479, tenantId=1146029695717560320, journalId=1146119944283992078, issueId=1261728796072358112, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1261732975071342784, tenantId=1146029695717560320, journalId=1146119944283992078, issueId=1261728796072358112, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=228, endPage=230, ext={CN=ArticleExt(id=1261728992386757154, articleId=1261728988259561985, tenantId=1146029695717560320, journalId=1146119944283992078, language=CN, title=高效液相色谱法检测易降解、基质复杂药物含量, columnId=1261728976733594600, journalTitle=实验室检测, columnName=分析与检测, runingTitle=null, highlight=null, articleAbstract=
目的 针对口服易降解、基质复杂药物（阿司匹林、布洛芬、硝苯地平）的含量检测难题，优化高效液相色谱法（high performance liquid chromatography, HPLC）检测条件并结合固相萃取（solid phase extraction, SPE）前处理技术，建立准确、灵敏的分析方法。
方法 以阿司匹林（水解敏感）、布洛芬（脂溶性干扰）、硝苯地平（光热不稳定）为研究对象，通过优化流动相组成（pH、有机相比例）、色谱柱类型及柱温，结合HLB SPE小柱净化消除基质效应；采用外标法定量，验证线性范围、回收率、精密度等指标。
结果 3种药物检测方法均达到药典要求：阿司匹林线性范围为5~500 μg/mL （r=0.9998），加样回收率为98.2%~101.5%｛日内/日间精密度［相对标准偏差（relative standard deviation, RSD）］≤1.5%｝；布洛芬线性范围为2~200 μg/mL （r=0.9997），回收率为97.8%~102.1%（RSD≤1.8%）；硝苯地平线性范围为1~100 μg/mL （r=0.9999），回收率为98.5%~101.8%（RSD≤1.2%）。实际样品检测结果显示，方法抗干扰能力强，适用于常规实验室质量控制。
结论 优化的HPLC-SPE方法可有效解决易降解/复杂基质口服药物的含量检测问题，操作简便、结果可靠，具有显著实验室应用价值。
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