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Objective Comparing two detection methods for chlamydial trachomatis in the genital tract (GeneXpertⓇ CT/NG and Roche® Cobas 4800 CT/NG), we summarize their respective advantages and disadvantages to provide references for the promotion and application of these two methods. Methods During a field survey conducted in a sample hospital, a convenience sampling method was employed to select 60 patients. Vaginal swabs were collected from these patients for testing using the GeneXpert® CT/NG system, while urine samples were collected for testing with the Roche® Cobas 4800 CT/NG system. Staff members were tasked with recording and compiling data related to the testing process, including the time taken for testing, the cost of testing, and the positive detection rates. This data was then used to compare the two testing methods. Results The testing time for Roche® Cobas 4800 CT/NG is 293 minutes, while the testing time for GeneXpertⓇ CT/NG is 92.95 minutes. In other words, the testing time for Roche® Cobas 4800 CT/NG is 3.15 times longer than that of GeneXpert® CT/NG; When the number of tests conducted in the sample hospital reaches 3000 cases, the cost of Roche® Cobas 4800 CT/NG testing is approximately 1/5 of that of GeneXpert® CT/NG testing. As the number of tests increases, when the volume reaches 200,000 cases, the unit cost of Roche® Cobas 4800 CT/NG testing is approximately 1/3 of the unit cost of GeneXpert® CT/NG testing; Among the 60 participants in this study, 11 cases of CT positive patients were detected by GeneXpert® CT/NG, with a CT positive rate of 0.183. Roche®Cobas 4800 CT/NG detected 10 cases, with a CT positive rate of 0.167. Conclusion The GeneXpert® CT/NG test has a significant time advantage, as it takes less time and provides faster results; The Roche® Cobas 4800 CT/NG test has a cost advantage, with lower detection costs; The GeneXpert® CT/ NG test has a higher positive detection rate.

, correspAuthors=You-Lin QIAO, authorNote=null, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yang TENG, Ting-Ting JIANG, Hai-Ling YE, Yu-Ping WANG, Xiang-Sheng CHEN, You-Lin QIAO), CN=ArticleExt(id=1156987936379064888, articleId=1156987906255573159, tenantId=1146029695717560320, journalId=1146119944283992078, language=CN, title=两种沙眼衣原体检测方法的比较, columnId=1152194691782505406, journalTitle=实验室检测, columnName=评价与分析, runingTitle=null, highlight=null, articleAbstract=

目的 比较两种生殖道沙眼衣原体检测方法(GeneXpert® CT/NG 和 Roche® Cobas 4800 CT/NG),总结各自优势劣势,从而为这两种方法的推广应用提供参考。方法 实地调查样本医院,利用方便抽样抽取 60 名患者, 分别采集阴道拭子用于GeneXpert $\circledast$ CT/NG 检测,尿液标本用于 Roche® CT/NG 检测,由工作人员记录、整理检测过程中的检测花费时间、检测成本以及检出阳性率等数据,从而比较两种检测方法。结果 Roche®Cobas 4800 CT/NG 的检测时间为 293 分钟, GeneXpert® CT/NG 检测时间为 92.95 分钟,即 Roche®Cobas 4800 CT/NG 检测是 GeneXpert® CT/NG 检测时间的 3.15 倍；当样本医院检测量在 3000 例时, Roche® Cobas 4800 CT/NG 检测成本约为 GeneXpert® CT/NG 检测成本的 1/5, 随着检测量的增大, 当检测量在 200000 例时, Roche® Cobas 4800 CT/NG 检测单位成本约为 GeneXpert@ CT/NG 检测单位成本的 1/3; 本研究 60 例参与者中, GeneXpert@ CT/NG 检测出 11 例 CT 阳性患者, CT 阳性率为 0.183。Roche®Cobas4800 CT/NG 检测出 10 例, CT 阳性率为 0.167。结论 GeneXpert@ CT/NG 检测更具有时间优势,耗费时间短,出结果更快；Roche®Cobas 4800 CT/NG 检测更具有成本优势,检测成本更低；GeneXpert@CT/NG 检测阳性检出率更高。

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滕扬,硕士,研究方向:衣原体感染。

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滕扬,硕士,研究方向:衣原体感染。

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乔友林,博士,教授,研究方向:肿瘤流行病学与人群预防,肿瘤筛查和分子标志物,HPV疫苗,职业癌的流行病学与预防。

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乔友林,博士,教授,研究方向:肿瘤流行病学与人群预防,肿瘤筛查和分子标志物,HPV疫苗,职业癌的流行病学与预防。

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Geneva: World Health Organization, 2021., articleTitle=Global progress report on HIV, viral hepatitis and sexually transmitted infections, 2021-Accountability for the global health sector strategies 2016-2021: actions for impact, refAbstract=null), Reference(id=1156987949704368974, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2021, volume=43, issue=05, pageStart=623, pageEnd=625, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=马娜, 张小斌, 刘春桃, journalName=皮肤病与性病, refType=null, unstructuredReference=马娜, 张小斌, 刘春桃, 等. 生殖道沙眼衣原体和淋球菌感染流行状况与防治[J]. 皮肤病与性病, 2021, 43: (05): 623-625., articleTitle=生殖道沙眼衣原体和淋球菌感染流行状况与防治, refAbstract=null), Reference(id=1156987949779866449, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2024, volume=24, issue=01, pageStart=405, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=ZHOU Q, LI J, LUO L, journalName=BMC Infect Dis, refType=null, unstructuredReference=ZHOU Q, LI J, LUO L, et al. 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BMJ Open, 2019, 9: (02): e026888., articleTitle=Diagnostic accuracy of the Xpert CT/NG and OSOM Trichomonas Rapid assays for point-of-care STI testing among young women in South Africa: a cross-sectional study, refAbstract=null), Reference(id=1156987950111216473, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2018, volume=126, issue=12, pageStart=907, pageEnd=912, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=JACOBSSON S, BOIKO I, GOLPARIAN D, journalName=APMIS, refType=null, unstructuredReference=JACOBSSON S, BOIKO I, GOLPARIAN D, et al. WHO laboratory validation of Xpert® CT/NG and Xpert® TV on the GeneXpert system verifies high performances[J]. APMIS, 2018, 126: (12): 907-912., articleTitle=WHO laboratory validation of Xpert® CT/NG and Xpert® TV on the GeneXpert system verifies high performances, refAbstract=null), Reference(id=1156987950174131035, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2024, volume=24, issue=Suppl 1, pageStart=277, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=SHEPHARD M, MATTHEWS S, KULARATNE R, journalName=BMC Infect Dis, refType=null, unstructuredReference=SHEPHARD M, MATTHEWS S, KULARATNE R, et al. Independent clinic-based evaluation of point-of-care testing for the screening of Chlamydia trachomatis, Neisseria gonorrhoea and Trichomonas vaginalis in women-at-risk in Australia, Guatemala, Morocco, and South Africa[J]. BMC Infect Dis, 2024, 24: (Suppl 1): 277., articleTitle=Independent clinic-based evaluation of point-of-care testing for the screening of Chlamydia trachomatis, Neisseria gonorrhoea and Trichomonas vaginalis in women-at-risk in Australia, Guatemala, Morocco, and South Africa, refAbstract=null), Reference(id=1156987950253822814, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2023, volume=12, issue=10, pageStart=1268, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=THAMMAJARUK N, RAMAUTARSING RA, HIRANSUTHIKUL A, journalName=Pathogens, refType=null, unstructuredReference=THAMMAJARUK N, RAMAUTARSING RA, HIRANSUTHIKUL A, et al. Pooled Pharyngeal, Rectal, and Urine Specimens for the Point-of-Care Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Lay Providers in Key Population-Led Health Services in Thailand[J]. Pathogens, 2023, 12: (10): 1268., articleTitle=Pooled Pharyngeal, Rectal, and Urine Specimens for the Point-of-Care Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Lay Providers in Key Population-Led Health Services in Thailand, refAbstract=null), Reference(id=1156987950333514592, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2022, volume=11, issue=null, pageStart=784610, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=HAN Y, SHI MQ, JIANG QP, journalName=Front Cell Infect Microbiol, refType=null, unstructuredReference=HAN Y, SHI MQ, JIANG QP, et al. Clinical Performance of the Xpert® CT/NG Test for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae: A Multicenter Evaluation in Chinese Urban Hospitals[J]. Front Cell Infect Microbiol, 2022, 11: 784610., articleTitle=Clinical Performance of the Xpert® CT/NG Test for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae: A Multicenter Evaluation in Chinese Urban Hospitals, refAbstract=null), Reference(id=1156987950396429155, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2016, volume=3, issue=01, pageStart=1021, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=GEIGER R, SMITH DM, LITTLE SJ, journalName=Austin. J. HIV AIDS Res, refType=null, unstructuredReference=GEIGER R, SMITH DM, LITTLE SJ, et al. Validation of the GeneXpert® CT/NG Assay for use with Male Pharyngeal and Rectal Swabs[J]. Austin. J. HIV AIDS Res, 2016, 3: (01): 1021., articleTitle=Validation of the GeneXpert® CT/NG Assay for use with Male Pharyngeal and Rectal Swabs, refAbstract=null), Reference(id=1156987950450955109, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, doi=null, pmid=null, pmcid=null, year=2021, volume=32, issue=02, pageStart=200, pageEnd=208, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=BERNARDINI S, PIERI M, CIOTTI M, journalName=EJIFCC, refType=null, unstructuredReference=BERNARDINI S, PIERI M, CIOTTI M. How Could POCT be a Useful Tool for Migrant and Refugee Health[J]. 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步骤名称	具体职能
问诊	医生通过系统询问获取患者病史资料
采样	医生采集阴道拭子
检测	检测样本, 检验步骤: 样品预处理 (人工)、加载样品 (人工)、加载试剂和检测 (自动)、样品卸载 (人工)
读取结果	读取检测结果

), ArticleFig(id=1156987948978754357, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, language=CN, label=表 1, caption=GeneXpert® CT/NG 检测各项步骤名称及具体职能表, figureFileSmall=null, figureFileBig=null, tableContent=

步骤名称	具体职能
问诊	医生通过系统询问获取患者病史资料
采样	医生采集阴道拭子
检测	检测样本, 检验步骤: 样品预处理 (人工)、加载样品 (人工)、加载试剂和检测 (自动)、样品卸载 (人工)
读取结果	读取检测结果

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步骤名称	具体职能
问诊	医生通过系统询问获取患者病史资料
采样	患者采集尿液
检测	检测样本, 检验步骤: 样品运输 (人工)、加载样品 (人工)、加载试剂 (人工)、样品和试剂预处理 (自动)、 密封和转移样品载板(人工)、检测(自动)、样品卸载(人工)
读取结果	读取检测结果

), ArticleFig(id=1156987949100389176, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, language=CN, label=表 2, caption=Roche® Cobas 4800 CT/NG 检测各项步骤名称及具体职能表, figureFileSmall=null, figureFileBig=null, tableContent=

步骤名称	具体职能
问诊	医生通过系统询问获取患者病史资料
采样	患者采集尿液
检测	检测样本, 检验步骤: 样品运输 (人工)、加载样品 (人工)、加载试剂 (人工)、样品和试剂预处理 (自动)、 密封和转移样品载板(人工)、检测(自动)、样品卸载(人工)
读取结果	读取检测结果

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	问诊	采样	检测	读取结果	总耗时
GeneXpert@CT/NG 检测	2.300	2.000	92.950	1.000	98.250
Roche®Cobas 4800 CT/NG 检测	2.300	3.120	293.000	1.000	299.420

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	问诊	采样	检测	读取结果	总耗时
GeneXpert@CT/NG 检测	2.300	2.000	92.950	1.000	98.250
Roche®Cobas 4800 CT/NG 检测	2.300	3.120	293.000	1.000	299.420

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检测量 (例)	GeneXpert® CT/NG 检测成本	Roche® Cobas 4800 CT/NG 检测成本	成本对比
3000	809.370	162.405	约 $1/5$
10000	461.750	117.815	约 $1/4$
30000	362.430	105.075	约 $1/3$
100000	327.668	100.616	约 $1/3$
200000	320.219	99.661	约 $1/3$

), ArticleFig(id=1156987949356241726, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, language=CN, label=表 4, caption=GeneXpert® CT/NG 检测和 Roche® Cobas 4800 CT/NG 检测成本对比分析表 (元), figureFileSmall=null, figureFileBig=null, tableContent=

检测量 (例)	GeneXpert® CT/NG 检测成本	Roche® Cobas 4800 CT/NG 检测成本	成本对比
3000	809.370	162.405	约 $1/5$
10000	461.750	117.815	约 $1/4$
30000	362.430	105.075	约 $1/3$
100000	327.668	100.616	约 $1/3$
200000	320.219	99.661	约 $1/3$

), ArticleFig(id=1156987949431739200, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, language=EN, label=Table 5, caption=Comparison of positive rates between two detection methods, figureFileSmall=null, figureFileBig=null, tableContent=

检测方法	检测量 (例)	CT 阳性( 例 )	阳性率
GeneXpert@CT/NG 检测	60	11	0.183
Roche® Cobas4800 CT/NG 检测	60	10	0.167

), ArticleFig(id=1156987949507236679, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156987906255573159, language=CN, label=表 5, caption=两种检测方法阳性率比较, figureFileSmall=null, figureFileBig=null, tableContent=

检测方法	检测量 (例)	CT 阳性( 例 )	阳性率
GeneXpert@CT/NG 检测	60	11	0.183
Roche® Cobas4800 CT/NG 检测	60	10	0.167

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