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Objective To investigate the distribution of Escherichia coli (E. coli) contamination in fruits sold in some cities and counties of Hainan Province, and to analyze the isolates for drug resistance, aiming at identifying the risk of foodborne E. coli and evaluating its potential impact on food safety. Methods Isolation and identification of E. coli in samples and detection of drug resistance of E. coli isolates by VITEK 2 method. Results A total of 51 strains of E. coli were isolated from 240 fruit samples, with a detection rate of 21.3%. 19 strains of E. coli were drug-resistant, with a resistance rate of 37.3%, including 7 single-resistant and 12 multi-resistant strains. Comparative analysis of the distribution of E. coli in different regions and fruits showed that fruit species and sampling locations had a significant effect on the distribution of Escherichia coli. Conclusion The distribution of E. coli in commercially available fruits is widespread and drug-resistant, the detection of pathogenic bacteria distribution and drug resistance in fruits should be strengthened, and this study also provides an important reference basis for food safety management and monitoring.
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*FENG Chen-Yun, Engineer, Hainan Academy of Inspection and Testing, Haikou 570203, China. E-mail:
378733138@qq.com, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Wei-Dao Yuan, Chun-Yan Feng, Cai-Xia Chen, Xue-Yin Wang, Chen-Yun Feng), CN=ArticleExt(id=1156967378736210029, articleId=1156967362164515797, tenantId=1146029695717560320, journalId=1146119944283992078, language=CN, title=海南市售水果中大肠埃希菌的分布特征及其耐药性分析, columnId=1152194691782505406, journalTitle=实验室检测, columnName=评价与分析, runingTitle=null, highlight=null, articleAbstract=
目的 调查海南省部分市县市售水果中大肠埃希菌(Escherichia coli)的污染分布情况,并对分离菌株进行耐药性分析,旨在识别食源性大肠埃希菌的风险并评估其对食品安全的潜在影响。方法 对样品中的大肠埃希菌进行分离鉴定,采用VITEK 2 法检测大肠埃希菌分离株的耐药性。结果 在240 份水果样品中共分离出大肠埃希菌51 株,检出率为21.3%,其中19 株大肠埃希菌具有耐药性,耐药率为37.3%,包括7 株单重耐药和12 株菌多重耐药。通过比较分析不同地区和不同水果中大肠埃希菌的分布情况,发现水果种类和采样地点对大肠埃希菌的分布有显著影响。结论 在市售水果中大肠埃希菌的分布比较广泛,且存在耐药情况,应加强对水果中病原菌分布和耐药性的检测,同时这项研究也为食品安全管理和监测提供了重要参考依据。
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袁维道,硕士,研究方向:药品、食品、化妆品微生物检验 |
冯晨韵,工程师,研究方向为药品、食品、化妆品微生物检验。
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Detection of Escherichia coli in different regions, figureFileSmall=sMQizihhKgKaoanjRksNKg==, figureFileBig=+gDBIuBp0Chmjkc08eVeWg==, tableContent=null), ArticleFig(id=1156967389649789260, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156967362164515797, language=CN, label=图 1, caption=
不同地区大肠埃希菌检出情况, figureFileSmall=sMQizihhKgKaoanjRksNKg==, figureFileBig=+gDBIuBp0Chmjkc08eVeWg==, tableContent=null), ArticleFig(id=1156967389712703821, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156967362164515797, language=EN, label=Table 1, caption=
Detection of Escherichia coli in different fruits, figureFileSmall=null, figureFileBig=null, tableContent=
| 水果名称 | 样品数量 | 检出数量 | 检出率(%) |
| 龙眼 | 30 | 12 | 40.0 |
| 芒果 | 30 | 2 | 6.7 |
| 沃柑 | 30 | 5 | 16.7 |
| 苹果 | 30 | 1 | 3.3 |
| 火龙果 | 30 | 7 | 23.3 |
| 香蕉 | 30 | 13 | 43.3 |
| 黑葡萄 | 30 | 9 | 30.0 |
| 百香果 | 30 | 2 | 6.7 |
| 总计 | 240 | 51 | 21.3 |
), ArticleFig(id=1156967389788201294, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156967362164515797, language=CN, label=表 1, caption=
不同水果中大肠埃希菌的检出情况, figureFileSmall=null, figureFileBig=null, tableContent=
| 水果名称 | 样品数量 | 检出数量 | 检出率(%) |
| 龙眼 | 30 | 12 | 40.0 |
| 芒果 | 30 | 2 | 6.7 |
| 沃柑 | 30 | 5 | 16.7 |
| 苹果 | 30 | 1 | 3.3 |
| 火龙果 | 30 | 7 | 23.3 |
| 香蕉 | 30 | 13 | 43.3 |
| 黑葡萄 | 30 | 9 | 30.0 |
| 百香果 | 30 | 2 | 6.7 |
| 总计 | 240 | 51 | 21.3 |
), ArticleFig(id=1156967389863698768, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156967362164515797, language=EN, label=Table 2, caption=
Analysis of antibiotic resistance of Escherichia coli $\left({n ={51}}\right)$, figureFileSmall=null, figureFileBig=null, tableContent=
| 抗生素 | 大肠埃希菌($n ={51}$) |
| R(%) | I (%) | S(%) |
| 氨苄西林(AM) | 17(33.3) | 0(0.0) | 34(66.7) |
| 氨苄西林/舒巴坦(SAM) | 7(13.7) | 7(13.7) | 37(72.5) |
| 哌拉西林 - 他唑巴坦(TZP) | 0(0.0) | 0(0.0) | 51(100.0) |
| 头孢唑啉(CZ) | 3(5.9) | 0(0.0) | ${48}\left({94.1}\right)$ |
| 头孢替坦(CTT) | 0(0.0) | 0(0.0) | 51(100.0) |
| 头孢他啶(CAZ) | 3(5.9) | 0(0.0) | 48(94.1) |
| 头孢曲松(CRO) | 3(5.9) | 0(0.0) | 48(94.1) |
| 头孢吡肟(FEP) | 4(7.8) | 0(0.0) | 47(92.2) |
| 氨曲南(ATM) | 3(5.9) | 0(0.0) | 48(94.1) |
| 厄他培南(ETP) | 0(0.0) | 0(0.0) | 51(100.0) |
| 亚胺培南(ETP) | 0 (0.0) | 0(0.0) | 51(100.0) |
| 阿米卡星(AN) | 0(0.0) | 0(0.0) | 51(100.0) |
| 庆大霉素(GM) | 0(0.0) | 0(0.0) | 51(100.0) |
| 妥布霉素(TM) | 0(0.0) | 0(0.0) | 51(100.0) |
| 环丙沙星(CIP) | 4(7.8) | 0(0.0) | 47(92.2) |
| 左旋氧氟沙星(LEV) | 4(7.8) | 0(0.0) | 47(92.2) |
| 呋喃妥因(FT) | 0(0.0) | 0(0.0) | 51(100.0) |
| 复方新诺明(SXT) | 3(5.9) | 0 (0.0) | 48(94.1) |
), ArticleFig(id=1156967389989527891, tenantId=1146029695717560320, journalId=1146119944283992078, articleId=1156967362164515797, language=CN, label=表 2, caption=
大肠埃希菌耐药性分析 (n=51), figureFileSmall=null, figureFileBig=null, tableContent=
| 抗生素 | 大肠埃希菌($n ={51}$) |
| R(%) | I (%) | S(%) |
| 氨苄西林(AM) | 17(33.3) | 0(0.0) | 34(66.7) |
| 氨苄西林/舒巴坦(SAM) | 7(13.7) | 7(13.7) | 37(72.5) |
| 哌拉西林 - 他唑巴坦(TZP) | 0(0.0) | 0(0.0) | 51(100.0) |
| 头孢唑啉(CZ) | 3(5.9) | 0(0.0) | ${48}\left({94.1}\right)$ |
| 头孢替坦(CTT) | 0(0.0) | 0(0.0) | 51(100.0) |
| 头孢他啶(CAZ) | 3(5.9) | 0(0.0) | 48(94.1) |
| 头孢曲松(CRO) | 3(5.9) | 0(0.0) | 48(94.1) |
| 头孢吡肟(FEP) | 4(7.8) | 0(0.0) | 47(92.2) |
| 氨曲南(ATM) | 3(5.9) | 0(0.0) | 48(94.1) |
| 厄他培南(ETP) | 0(0.0) | 0(0.0) | 51(100.0) |
| 亚胺培南(ETP) | 0 (0.0) | 0(0.0) | 51(100.0) |
| 阿米卡星(AN) | 0(0.0) | 0(0.0) | 51(100.0) |
| 庆大霉素(GM) | 0(0.0) | 0(0.0) | 51(100.0) |
| 妥布霉素(TM) | 0(0.0) | 0(0.0) | 51(100.0) |
| 环丙沙星(CIP) | 4(7.8) | 0(0.0) | 47(92.2) |
| 左旋氧氟沙星(LEV) | 4(7.8) | 0(0.0) | 47(92.2) |
| 呋喃妥因(FT) | 0(0.0) | 0(0.0) | 51(100.0) |
| 复方新诺明(SXT) | 3(5.9) | 0 (0.0) | 48(94.1) |
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