Article(id=1216517522879332543, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1216517514570417012, articleNumber=null, orderNo=null, doi=10.19812/j.cnki.jfsq11-5956/ts.20250219003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1739894400000, receivedDateStr=2025-02-19, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1767969979256, onlineDateStr=2026-01-09, pubDate=1755187200000, pubDateStr=2025-08-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1767969979256, onlineIssueDateStr=2026-01-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1767969979256, creator=13701087609, updateTime=1767969979256, updator=13701087609, issue=Issue{id=1216517514570417012, tenantId=1146029695717560320, journalId=1149652044408987649, year='2025', volume='16', issue='15', pageStart='1', pageEnd='322', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1767969977276, creator=13701087609, updateTime=1768211590858, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1217530915467743720, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1216517514570417012, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1217530915467743721, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1216517514570417012, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=118, endPage=125, ext={EN=ArticleExt(id=1216517524225704206, articleId=1216517522879332543, tenantId=1146029695717560320, journalId=1149652044408987649, language=EN, title=Rapid colorimetric detection of histamine in fish based on the oxidase activity of Ag₃PO₄ nanozyme, columnId=1216517518575980656, journalTitle=Journal of Food Safety & Quality, columnName=Special Topic: Application of Biosensors in Food Safety Detection, runingTitle=null, highlight=null, articleAbstract=

Objective To establish a rapid colorimetric method for the detection of histamine in fish samples based on the oxidase activity of Ag₃PO₄ nanozyme. Methods Ag₃PO₄ nanoparticles were prepared by the hydrothermal method. Utilizing their oxidase catalytic activity, with CH₃COONa as the buffer system and 3,3',5,5'-tetramethylbenzidine as the oxidation substrate, a rapid colorimetric detection method for histamine in fish was established. Results The detection time of this method was about 100 seconds. The limit of detection was 0.58 mg/L, indicating high detection sensitivity. In the detection of multiple competitive targets, this method showed good specificity for histamine molecules. In the spiked experiments on actual samples, the spiked recovery rate of this method ranged from 104.24% to 108.30%, and the relative standard deviations were from 1.81% to 3.44%, which had good detection accuracy. Conclusion This method demonstrates the advantages of being intuitive, rapid, highly sensitive and having good specificity. It can adapt to the complex daily detection environment and provides a new idea for the detection of histamine in fish meat.

, correspAuthors=Bin SHI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Bin SHI, Li JIANG, Yong-Fu LI, Yao-Qi RAN, Qi-Qi LUO), CN=ArticleExt(id=1216517532048081588, articleId=1216517522879332543, tenantId=1146029695717560320, journalId=1149652044408987649, language=CN, title=基于Ag₃PO₄纳米酶氧化活性快速比色检测鱼肉中组胺, columnId=1216517518756335730, journalTitle=食品安全质量检测学报, columnName=专题：生物传感器在食品安全检测中的应用, runingTitle=null, highlight=null, articleAbstract=

目的 建立基于Ag₃PO₄纳米酶氧化活性快速比色法检测鱼肉样品中组胺的方法。方法 通过水热法制备了Ag₃PO₄纳米颗粒, 并利用其氧化酶催化活性, 以CH₃COONa为缓冲体系, 以3,3',5,5'-四甲基联苯胺作为氧化底物, 建立了一种鱼肉中组胺的快速比色检测方法。结果 该方法检测时间在100 s左右, 检出限为0.58 mg/L, 具有较高的检测灵敏度; 在多种竞争性靶标检测中, 该方法表现出对组胺分子的良好的特异性; 在实际样品加标实验中, 该方法加标回收率为104.24%~108.30%, 相对标准偏差为1.81%~3.44%, 具有良好的检测准确性。结论 该方法表现出了直观、快速、灵敏度高、特异性好的优点, 能适应日常复杂的检测环境, 为鱼肉中组胺的检测提供了一种新的思路。

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Preparation of silver phosphate-polyethersulfone composite membrane and its adsorptive removal of bromide ions[J]. New Chemical Materials, 2022, 50(10): 81-85., articleTitle=Preparation of silver phosphate-polyethersulfone composite membrane and its adsorptive removal of bromide ions, refAbstract=null)], funds=[Fund(id=1217127908661973178, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, awardId=黔科合支撑[2021]一般108, language=CN, fundingSource=贵州省科技支撑项目(黔科合支撑[2021]一般108), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1217127900655047436, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, xref=null, ext=[AuthorCompanyExt(id=1217127900663436045, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, companyId=1217127900655047436, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Guizhou Academy of Agricultural Science, Guizhou Institute of integrated Agricultural Development, Guiyang 550009, China), AuthorCompanyExt(id=1217127900671824657, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, companyId=1217127900655047436, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=贵州省农业科学院, 贵州省现代农业发展研究所, 贵阳 550009)])], figs=[ArticleFig(id=1217127905277169696, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Fig.1, caption=Characterization diagram of the prepared Ag₃PO₄ nanomaterial, figureFileSmall=tk7ZT8TOAe3ZWK8uVu0Q/Q==, figureFileBig=IZSXjFegSPMVZazbmpJWKA==, tableContent=null), ArticleFig(id=1217127905402998829, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=图1, caption=制备Ag₃PO₄纳米材料表征图

注: A. 制备Ag₃PO₄纳米材料5000倍电镜图; B. 制备Ag₃PO₄纳米材料20000倍电镜图; C. Ag₃PO₄晶体的标准卡XRD图谱; D. 制备Ag₃PO₄纳米材料XRD图谱。

, figureFileSmall=tk7ZT8TOAe3ZWK8uVu0Q/Q==, figureFileBig=IZSXjFegSPMVZazbmpJWKA==, tableContent=null), ArticleFig(id=1217127906774536251, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Fig.2, caption=Catalytic effects of Ag₃PO₄ with different concentrations, figureFileSmall=e8nIXQlsa1CuZOzHnXuTRw==, figureFileBig=dUK0bx0xXMjejTf7F+wyXQ==, tableContent=null), ArticleFig(id=1217127906912948291, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=图2, caption=不同浓度Ag₃PO₄催化效果

注: 1: 0 mg/L; 2: 10 mg/L; 3: 15 mg/L; 4: 20 mg/L; 5: 25 mg/L; 6: 30 mg/L。

, figureFileSmall=e8nIXQlsa1CuZOzHnXuTRw==, figureFileBig=dUK0bx0xXMjejTf7F+wyXQ==, tableContent=null), ArticleFig(id=1217127907059748941, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Fig.3, caption=Schematic diagram of the principle for detecting histamine by Ag₃PO₄, figureFileSmall=4iAS9LivO9BI2TP1kJrjZw==, figureFileBig=Uu0cNtuDzIlJXuD1WQk/RA==, tableContent=null), ArticleFig(id=1217127907206549589, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=图3, caption=Ag₃PO₄检测组胺原理示意图, figureFileSmall=4iAS9LivO9BI2TP1kJrjZw==, figureFileBig=Uu0cNtuDzIlJXuD1WQk/RA==, tableContent=null), ArticleFig(id=1217127907311407195, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Fig.4, caption=Detection results of histamine in the Ag₃PO₄ system under different conditions, figureFileSmall=jRNE5UimLd0tOQ+E5JYQYQ==, figureFileBig=acJynm6ykwJWZKTowecRig==, tableContent=null), ArticleFig(id=1217127907403681888, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=图4, caption=不同条件Ag₃PO₄体系对组胺的检测结果

注: 1: 纯净水+TMB; 2: 20 mg/L Ag₃PO₄+TMB; 3: 2+100 μmol/L组胺; 4: 2+50 μmol/L组胺; 5: 2+20 μmol/L组胺; 6: 2+5 μmol/L组胺。

, figureFileSmall=jRNE5UimLd0tOQ+E5JYQYQ==, figureFileBig=acJynm6ykwJWZKTowecRig==, tableContent=null), ArticleFig(id=1217127907483373670, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Fig.5, caption=Influences of different conditions on the detection system, figureFileSmall=ZTxYe//b+t8epbwjPOZ4PA==, figureFileBig=gUwTvTLE8aAeq5ZoWmV5XA==, tableContent=null), ArticleFig(id=1217127907630174318, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=图5, caption=不同条件对检测体系的影响, figureFileSmall=ZTxYe//b+t8epbwjPOZ4PA==, figureFileBig=gUwTvTLE8aAeq5ZoWmV5XA==, tableContent=null), ArticleFig(id=1217127907764392054, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Fig.6, caption=ΔA650 changes in the detection of histamine with different concentrations, figureFileSmall=mpPFk4DtHJmR6WJTKaPO/A==, figureFileBig=gVdGdZ/oKEpshq/v8vo9kw==, tableContent=null), ArticleFig(id=1217127907911192704, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=图6, caption=不同浓度组胺的检测的ΔA650变化, figureFileSmall=mpPFk4DtHJmR6WJTKaPO/A==, figureFileBig=gVdGdZ/oKEpshq/v8vo9kw==, tableContent=null), ArticleFig(id=1217127908016050314, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Fig.7, caption=Effects of different competitive substances on the detection, figureFileSmall=GU8djXBkT7tAAJciJt7KzQ==, figureFileBig=Jv7PsvSLBf0fW4wDtbbfmg==, tableContent=null), ArticleFig(id=1217127908129296531, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=图7, caption=不同竞争性物质对检测的影响

注: A. 添加200 μmol/L浓度不同的竞争性物质体系颜色变化。B. 添加200 μmol/L浓度不同的竞争性物质体系ΔA650变化。

, figureFileSmall=GU8djXBkT7tAAJciJt7KzQ==, figureFileBig=Jv7PsvSLBf0fW4wDtbbfmg==, tableContent=null), ArticleFig(id=1217127908246737053, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=EN, label=Table 1, caption=

Detection table of spiked recovery of histamine in fish samples

, figureFileSmall=null, figureFileBig=null, tableContent=

加标量 /(μmol/L)	本方法			高效液相色谱法
	测定值 /(μmol/L)	平均回收率 /%	相对标准偏差/%	测定值 /(mg/kg)	平均回收率 /%	相对标准偏差/%
5.0	5.26	105.20	1.81	5.38	107.60	1.86
10.0	10.81	108.10	2.81	11.62	116.20	3.18
30.0	32.49	108.30	3.44	28.01	93.34	3.12
50.0	52.12	104.24	2.26	52.45	104.90	2.18

), ArticleFig(id=1217127908376760486, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1216517522879332543, language=CN, label=表1, caption=

鱼肉样品中组胺加标回收检测表

, figureFileSmall=null, figureFileBig=null, tableContent=

加标量 /(μmol/L)	本方法			高效液相色谱法
	测定值 /(μmol/L)	平均回收率 /%	相对标准偏差/%	测定值 /(mg/kg)	平均回收率 /%	相对标准偏差/%
5.0	5.26	105.20	1.81	5.38	107.60	1.86
10.0	10.81	108.10	2.81	11.62	116.20	3.18
30.0	32.49	108.30	3.44	28.01	93.34	3.12
50.0	52.12	104.24	2.26	52.45	104.90	2.18

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