Article(id=1153429496670703859, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1153429493357203682, articleNumber=null, orderNo=null, doi=10.19812/j.cnki.jfsq11-5956/ts.20240827003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1724688000000, receivedDateStr=2024-08-27, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1752928621690, onlineDateStr=2025-07-19, pubDate=1741968000000, pubDateStr=2025-03-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1752928621690, onlineIssueDateStr=2025-07-19, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1752928621690, creator=13701087609, updateTime=1752928621690, updator=13701087609, issue=Issue{id=1153429493357203682, tenantId=1146029695717560320, journalId=1149652044408987649, year='2025', volume='16', issue='5', pageStart='1', pageEnd='326', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=0, createTime=1752928620900, creator=13701087609, updateTime=1758690311058, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1177595773500932351, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1153429493357203682, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1177595773500932352, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1153429493357203682, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=215, endPage=221, ext={EN=ArticleExt(id=1153429497140465923, articleId=1153429496670703859, tenantId=1146029695717560320, journalId=1149652044408987649, language=EN, title=Determination of 6 kinds of Sudan dyes in
Capsicum annuum powder by deep eutectic solvent liquid-liquid microextraction-high performance liquid chromatography, columnId=1151895321388347923, journalTitle=Journal of Food Safety & Quality, columnName=Food Analysis and Detection, runingTitle=null, highlight=null, articleAbstract=
Objective To establish an analytical method for the determination of 6 kinds of Sudan dyes in Capsicum annuum powder based on liquid-liquid microextraction with deep eutectic solvents (DES) combined with high performance liquid chromatography. Methods Sudan red I, Sudan red II, Sudan red III, Sudan red IV, Sudan red 7B and Sudan red G in Capsicum annuum powder were extracted by liquid-liquid microextraction. The extract was filtered through a microporous membrane and then determined by high performance liquid chromatography with external standard method for quantification. The effects of DES dilution ratio, DES molar ratio, DES addition amount, extraction time, and extraction method on the extraction efficiency of 6 kinds of Sudan dyes were investigated. Results The results showed that the optimal method conditions were: Dilution ratio of DES 5 times, molar ratio of DES 1:2.5, addition amount of DES 600 μL, extraction time 50 s, and extraction method was vortex extraction. Under these conditions, the established equation had a good linear relationship in the range of mass concentration 0.1-50.0 mg/L, the correlation coefficients were all greater than 0.999, the limits of detection were 0.03-0.20 mg/kg, and the limits of quantitative were 0.10-1.00 mg/kg. The recovery rates of Sudan red G, Sudan red I, and Sudan red II were higher, ranging from 71.6% to 117.5%, with relative standard deviations of 0.6% to 4.7%. Conclusion The established method is simple, efficient, and environmentally friendly, and can be used for rapid detection of 6 kinds of Sudan dyes in Capsicum annuum.
, correspAuthors=Zhao-Jing XIAO, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Zong-Qin RAN, Wen-Qin YU, Qiong CAI, Li-Li YAO, Xiao-Yan TIAN, Zhao-Jing XIAO), CN=ArticleExt(id=1153429526513177503, articleId=1153429496670703859, tenantId=1146029695717560320, journalId=1149652044408987649, language=CN, title=低共熔溶剂液-液微萃取-高效液相色谱法检测辣椒粉中6种苏丹红色素含量, columnId=1151895321958773274, journalTitle=食品安全质量检测学报, columnName=食品分析与检测, runingTitle=null, highlight=null, articleAbstract=
目的 建立一种基于低共熔溶剂(deep eutectic solvent, DES)萃取结合高效液相色谱法快速检测辣椒粉中6种苏丹红的方法。方法 利用液-液微萃取方法提取辣椒粉中的苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ、苏丹红7B、苏丹红G。将提取液通过微孔滤膜过滤后进行高效液相色谱法测定, 外标法定量。考察DES稀释倍数、DES摩尔比、DES用量、萃取时间和萃取方式对6种苏丹红色素萃取效果的影响。结果 最优方法条件为DES稀释倍数为5倍、DES摩尔比1:2.5、DES用量600 μL、萃取时间50 s、萃取方式为涡旋萃取, 在此条件下, 建立的方程在质量浓度0.1~50.0 mg/L范围内, 线性关系良好, 相关系数均大于0.999, 检出限为0.03~0.20 mg/L, 定量限为0.10~1.00 mg/L。其中苏丹红G、苏丹红Ⅰ和苏丹红Ⅱ的回收率较高, 为71.6%~117.5%, 相对标准偏差为0.6%~4.7%。结论 本研究建立的方法操作简便、高效、绿色环保, 可对辣椒粉中6种苏丹红进行快速检测。
, correspAuthors=肖昭竞, authorNote=null, correspAuthorsNote=
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, authorsList=冉宗勤, 余文琴, 蔡琼, 姚莉莉, 田小艳, 肖昭竞)}, authors=[Author(id=1177619554688713497, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=940719699@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1177619554751628059, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, authorId=1177619554688713497, language=EN, stringName=Zong-Qin RAN, firstName=Zong-Qin, middleName=null, lastName=RAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Chongqing Academy of Metrology and Quality Inspection, Chongqing 401123, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1177619554806154012, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, authorId=1177619554688713497, language=CN, stringName=冉宗勤, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=重庆市计量质量检测研究院, 重庆 401123, bio={"content":"
冉宗勤(1993—), 女, 硕士, 工程师, 主要研究方向为食品安全与检测。E-mail: 940719699@qq.com
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冉宗勤(1993—), 女, 硕士, 工程师, 主要研究方向为食品安全与检测。E-mail: 940719699@qq.com
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Capsicum annuum powder), Keyword(id=1177619556597121851, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, orderNo=1, keyword=低共熔溶剂), Keyword(id=1177619556668425020, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, orderNo=2, keyword=液-液微萃取), Keyword(id=1177619556748116797, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, orderNo=3, keyword=高效液相色谱法), Keyword(id=1177619556823614270, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, orderNo=4, keyword=苏丹红), Keyword(id=1177619556886528831, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, orderNo=5, keyword=辣椒粉)], refs=[Reference(id=1177619558807520085, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, doi=null, pmid=null, pmcid=null, year=2021, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=苏非语, journalName=市售火锅底料中苏丹红残留量评价及检测方法研究, refType=null, unstructuredReference=苏非语.
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9(25): 3810-3818., articleTitle=Electrolyte-assisted microemulsion breaking in vortexagitated solidified floating organic drop microextraction for preconcentration and analysis of sudan dyes in chili products, refAbstract=null)], funds=[Fund(id=1177619558601999188, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, awardId=CQSJKJ2020002, language=CN, fundingSource=重庆市市场监督管理局项目(CQSJKJ2020002), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1177619554625798933, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, xref=null, ext=[AuthorCompanyExt(id=1177619554629993238, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, companyId=1177619554625798933, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Chongqing Academy of Metrology and Quality Inspection, Chongqing 401123, China), AuthorCompanyExt(id=1177619554638381847, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, companyId=1177619554625798933, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=重庆市计量质量检测研究院, 重庆 401123)])], figs=[ArticleFig(id=1177619557096244032, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Fig.1, caption=
Chromatograms of different mobile phase, figureFileSmall=Iu7YuudyA+yoNLYvS96odQ==, figureFileBig=tS3mcKnim1/lYfkJw2JMyA==, tableContent=null), ArticleFig(id=1177619557209490241, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=图1, caption=
不同洗脱条件下的色谱图 注: a、b、c分别为表1中不同洗脱方案对应的色谱图。1~6分别为苏丹红G、苏丹红I、苏丹红II、苏丹红III、苏丹红7B、苏丹红IV。
, figureFileSmall=Iu7YuudyA+yoNLYvS96odQ==, figureFileBig=tS3mcKnim1/lYfkJw2JMyA==, tableContent=null), ArticleFig(id=1177619557280793410, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Fig.2, caption=
Chromatogram of standard product at different dilution multiples, figureFileSmall=YMFuVhm9S6y/wRIT8UnIaQ==, figureFileBig=6DA/VQXtfC8yTt3lbp7yMA==, tableContent=null), ArticleFig(id=1177619557385651011, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=图2, caption=
不同稀释倍数下标准品色谱图, figureFileSmall=YMFuVhm9S6y/wRIT8UnIaQ==, figureFileBig=6DA/VQXtfC8yTt3lbp7yMA==, tableContent=null), ArticleFig(id=1177619557482120004, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Fig.3, caption=
Effects of molar ratio on recovery rates, figureFileSmall=Q6gcl83sf64OSEt/nDBKUA==, figureFileBig=ri52etHs942N5bkB6dYHog==, tableContent=null), ArticleFig(id=1177619557595366213, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=图3, caption=
摩尔比对回收率的影响, figureFileSmall=Q6gcl83sf64OSEt/nDBKUA==, figureFileBig=ri52etHs942N5bkB6dYHog==, tableContent=null), ArticleFig(id=1177619557670863686, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Fig.4, caption=
Effects of DES dosage on recovery rates, figureFileSmall=eQjHqrMlP+f65fuCs8caIw==, figureFileBig=w2H90rKQtkZ7OpMBCxixZw==, tableContent=null), ArticleFig(id=1177619557733778247, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=图4, caption=
DES用量对回收率的影响, figureFileSmall=eQjHqrMlP+f65fuCs8caIw==, figureFileBig=w2H90rKQtkZ7OpMBCxixZw==, tableContent=null), ArticleFig(id=1177619557775721288, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Fig.5, caption=
Effects of extraction method and time on recovery rate, figureFileSmall=KxV1nvz0UxzFi1NiakdONA==, figureFileBig=d08B1sSUGDzrIj1LOPzY3w==, tableContent=null), ArticleFig(id=1177619557842830153, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=图5, caption=
萃取方式及时间对回收率的影响, figureFileSmall=KxV1nvz0UxzFi1NiakdONA==, figureFileBig=d08B1sSUGDzrIj1LOPzY3w==, tableContent=null), ArticleFig(id=1177619557914133322, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Table 1, caption=
Composition and ratio of the DES
, figureFileSmall=null, figureFileBig=null, tableContent=
| DES编号 | 甲基三辛基氯化铵与油酸摩尔比 |
| DES-1 | 1.0:1.0 |
| DES-2 | 1.0:1.5 |
| DES-3 | 1.0:2.0 |
| DES-4 | 1.0:2.5 |
| DES-5 | 1.0:3.0 |
), ArticleFig(id=1177619557972853579, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=表1, caption=
DES的组成及配比
, figureFileSmall=null, figureFileBig=null, tableContent=
| DES编号 | 甲基三辛基氯化铵与油酸摩尔比 |
| DES-1 | 1.0:1.0 |
| DES-2 | 1.0:1.5 |
| DES-3 | 1.0:2.0 |
| DES-4 | 1.0:2.5 |
| DES-5 | 1.0:3.0 |
), ArticleFig(id=1177619558023185228, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Table 2, caption=
Different gradient elution conditions
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| 方案 | 梯度洗脱程序 |
| a | 时间/min | 0.01 | 10.00 | 25.00 | 32.00 | 35.00 | 40.00 |
| 流动相A/% | 75 | 75 | 100 | 100 | 75 | 75 |
| 流动相B/% | 25 | 25 | 0 | 0 | 25 | 25 |
| b | 时间/min | 0.01 | 10.00 | 25.00 | 32.00 | 35.00 | 45.00 |
| 流动相A/% | 75 | 75 | 100 | 100 | 100 | 75 |
| 流动相B/% | 25 | 25 | 0 | 0 | 0 | 25 |
| c | 时间/min | 4.00 | 8.00 | 20.00 | 25.00 | | |
| 流动相A/% | 80 | 100 | 100 | 80 | | |
| 流动相B/% | 20 | 0 | 0 | 20 | | |
), ArticleFig(id=1177619558098682701, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=表2, caption=
不同梯度洗脱条件
, figureFileSmall=null, figureFileBig=null, tableContent=
| 方案 | 梯度洗脱程序 |
| a | 时间/min | 0.01 | 10.00 | 25.00 | 32.00 | 35.00 | 40.00 |
| 流动相A/% | 75 | 75 | 100 | 100 | 75 | 75 |
| 流动相B/% | 25 | 25 | 0 | 0 | 25 | 25 |
| b | 时间/min | 0.01 | 10.00 | 25.00 | 32.00 | 35.00 | 45.00 |
| 流动相A/% | 75 | 75 | 100 | 100 | 100 | 75 |
| 流动相B/% | 25 | 25 | 0 | 0 | 0 | 25 |
| c | 时间/min | 4.00 | 8.00 | 20.00 | 25.00 | | |
| 流动相A/% | 80 | 100 | 100 | 80 | | |
| 流动相B/% | 20 | 0 | 0 | 20 | | |
), ArticleFig(id=1177619558161597262, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Table 3, caption=
Separation degree under different elution conditions
, figureFileSmall=null, figureFileBig=null, tableContent=
| 化合物 | 方案a | 方案b | 方案c |
| 苏丹红G | 2.576 | 2.529 | 2.280 |
| 苏丹红Ⅰ | 3.042 | 3.015 | 2.630 |
| 苏丹红Ⅱ | 17.091 | 15.107 | 10.877 |
| 苏丹红Ⅲ | 11.855 | 11.194 | 6.899 |
| 苏丹红7B | 18.894 | 16.226 | 12.615 |
| 苏丹红Ⅳ | 0.458 | 1.285 | 1.514 |
), ArticleFig(id=1177619558241289039, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=表3, caption=
不同洗脱条件下的分离度
, figureFileSmall=null, figureFileBig=null, tableContent=
| 化合物 | 方案a | 方案b | 方案c |
| 苏丹红G | 2.576 | 2.529 | 2.280 |
| 苏丹红Ⅰ | 3.042 | 3.015 | 2.630 |
| 苏丹红Ⅱ | 17.091 | 15.107 | 10.877 |
| 苏丹红Ⅲ | 11.855 | 11.194 | 6.899 |
| 苏丹红7B | 18.894 | 16.226 | 12.615 |
| 苏丹红Ⅳ | 0.458 | 1.285 | 1.514 |
), ArticleFig(id=1177619558295814992, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Table 4, caption=
Calibration curve equations, limits of detection and limits of quantification of 6 kinds of Sudan dyes
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| 组分 | 线性回归
方程 | 相关
系数 | 检出限
/(mg/L) | 定量限
/(mg/L) |
| 苏丹红Ⅰ | Y=66593X+1451 | 0.9999 | 0.03 | 0.10 |
| 苏丹红Ⅱ | Y=57856X-1316 | 0.9999 | 0.05 | 0.15 |
| 苏丹红Ⅲ | Y=13843X-1336 | 0.9999 | 0.15 | 1.00 |
| 苏丹红Ⅳ | Y=30314X-170 | 0.9998 | 0.20 | 1.00 |
| 苏丹红7B | Y=94825X-969 | 0.9999 | 0.05 | 0.10 |
| 苏丹红G | Y=53686X-906 | 0.9999 | 0.03 | 0.10 |
), ArticleFig(id=1177619558354535249, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=表4, caption=
6种苏丹红染料的校准曲线方程、相关系数、检出限和定量限结果
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| 组分 | 线性回归
方程 | 相关
系数 | 检出限
/(mg/L) | 定量限
/(mg/L) |
| 苏丹红Ⅰ | Y=66593X+1451 | 0.9999 | 0.03 | 0.10 |
| 苏丹红Ⅱ | Y=57856X-1316 | 0.9999 | 0.05 | 0.15 |
| 苏丹红Ⅲ | Y=13843X-1336 | 0.9999 | 0.15 | 1.00 |
| 苏丹红Ⅳ | Y=30314X-170 | 0.9998 | 0.20 | 1.00 |
| 苏丹红7B | Y=94825X-969 | 0.9999 | 0.05 | 0.10 |
| 苏丹红G | Y=53686X-906 | 0.9999 | 0.03 | 0.10 |
), ArticleFig(id=1177619558413255506, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=EN, label=Table 5, caption=
Average recoveries and precisions of 6 kinds of Sudan dyes in Capsicum annuum powder (n=6)
, figureFileSmall=null, figureFileBig=null, tableContent=
| 组分 | 0.5 mg/kg | 2.5 mg/kg | 5.0 mg/kg |
回收率
/% | 相对标准
偏差/% | 回收率
/% | 相对标准偏差/% | 回收率
/% | 相对标准偏差/% |
| 苏丹红G | 117.5 | 0.6 | 75.1 | 1.8 | 71.6 | 3.2 |
| 苏丹红Ⅰ | 107.0 | 1.1 | 84.2 | 1.7 | 76.7 | 4.7 |
| 苏丹红Ⅱ | 81.0 | 0.7 | 89.2 | 4.6 | 81.1 | 4.0 |
| 苏丹红Ⅲ | 71.3 | 2.8 | 60.2 | 4.9 | 50.0 | 5.2 |
| 苏丹红7B | 45.2 | 4.9 | 35.6 | 6.1 | 32.1 | 11.1 |
| 苏丹红Ⅳ | 57.7 | 0.5 | 46.7 | 3.8 | 30.6 | 6.6 |
), ArticleFig(id=1177619558476170067, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153429496670703859, language=CN, label=表5, caption=
辣椒粉中6种苏丹红染料的加标回收率及精密度(n=6)
, figureFileSmall=null, figureFileBig=null, tableContent=
| 组分 | 0.5 mg/kg | 2.5 mg/kg | 5.0 mg/kg |
回收率
/% | 相对标准
偏差/% | 回收率
/% | 相对标准偏差/% | 回收率
/% | 相对标准偏差/% |
| 苏丹红G | 117.5 | 0.6 | 75.1 | 1.8 | 71.6 | 3.2 |
| 苏丹红Ⅰ | 107.0 | 1.1 | 84.2 | 1.7 | 76.7 | 4.7 |
| 苏丹红Ⅱ | 81.0 | 0.7 | 89.2 | 4.6 | 81.1 | 4.0 |
| 苏丹红Ⅲ | 71.3 | 2.8 | 60.2 | 4.9 | 50.0 | 5.2 |
| 苏丹红7B | 45.2 | 4.9 | 35.6 | 6.1 | 32.1 | 11.1 |
| 苏丹红Ⅳ | 57.7 | 0.5 | 46.7 | 3.8 | 30.6 | 6.6 |
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