Article(id=1153986582030836387, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1153986579971429187, articleNumber=null, orderNo=null, doi=10.19812/j.cnki.jfsq11-5956/ts.20241218008, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1734451200000, receivedDateStr=2024-12-18, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1753061441190, onlineDateStr=2025-07-21, pubDate=1740412800000, pubDateStr=2025-02-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1753061441190, onlineIssueDateStr=2025-07-21, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1753061441190, creator=13701087609, updateTime=1753061441190, updator=13701087609, issue=Issue{id=1153986579971429187, tenantId=1146029695717560320, journalId=1149652044408987649, year='2025', volume='16', issue='4', pageStart='1', pageEnd='320', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=0, createTime=1753061440699, creator=13701087609, updateTime=1758783495950, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1177986619249406427, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1153986579971429187, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1177986619249406428, tenantId=1146029695717560320, journalId=1149652044408987649, issueId=1153986579971429187, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=44, endPage=52, ext={EN=ArticleExt(id=1153986582483821223, articleId=1153986582030836387, tenantId=1146029695717560320, journalId=1149652044408987649, language=EN, title=Research progress on determination of pollutants in food by liquid chromatography-high resolution mass spectrometry, columnId=1153429495274000613, journalTitle=Journal of Food Safety & Quality, columnName=Special Topic: Research and Detection of Pesticide and Veterinary Drug Residue, runingTitle=null, highlight=null, articleAbstract=
There are many types of pollutants in food, and exposure involves multiple stages such as raw material, processing, transportation, storage, packaging, and sales. With the improvement of people's requirements for the healthy, nutritive and safe condiments, the pollutants in food have received more and more attention. Liquid chromatography-high resolution mass spectrometry (LC-HRMS) combines the separation ability of liquid chromatography with the advantages of high resolution mass spectrometry, such as wide mass range, fast scanning speed, and high sensitivity, making it an important means in the field of food safety analysis. This review summarized the technical characteristics of quadrupole-time of flight mass spectrometry and quadrupole-orbitrap mass spectrometry, as well as the research progress in the detection of pollutants in food using LC-HRMS technology over the past 5 years. The article focused on the application of LC-HRMS in the targeted and non-targeted determination of pesticides residues, veterinary drugs and their metabolites, food additives, illegal additives and mycotoxins, and prospected the development trend. Aiming to provide reference for the detection of pollutants in food in the future.
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食品中污染物种类繁多, 且暴露涉及食品原料、加工、运输、储藏、包装和销售等多个环节, 随着人们对食品健康营养安全需求的提高, 食品中的污染物问题受到越来越多的关注。液相色谱-串联高分辨质谱(liquid chromatography-high resolution mass spectrometry, LC-HRMS)技术结合了液相色谱的分离能力和高分辨质谱的质量范围宽、扫描速度快、灵敏度高等优点, 成为食品安全分析领域的重要手段。本文综述了四极杆/飞行时间质谱、四极杆/静电场轨道阱质谱技术的特点以及近5年来LC-HRMS技术在食品中污染物检测方面的研究进展, 重点讨论了LC-HRMS靶向、非靶向测定食品中农药残留、兽药及其代谢物、非法添加物、食品添加剂、毒素等方面的应用, 并对其未来发展趋势进行了展望, 旨在为今后食品中污染物的检测提供参考。
, correspAuthors=吴彦蕾, authorNote=null, correspAuthorsNote=
* 吴彦蕾(1985—), 女, 硕士, 工程师, 主要研究方向为食品安全与检测。E-mail: eva1985_r @qq.com
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, authorsList=苏敏, 李红丽, 白亚敏, 黄大亮, 刘元, 吴彦蕾)}, authors=[Author(id=1177985600511689702, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=398568461@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1177985600633324523, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, authorId=1177985600511689702, language=EN, stringName=Min SU, firstName=Min, middleName=null, lastName=SU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Chongqing Institute for Food and Drug Control, Key Laboratory of Condiment Supervision Technology, State Administration for Market Regulation, Chongqing 401121, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1177985600683656173, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, authorId=1177985600511689702, language=CN, stringName=苏敏, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=重庆市食品药品检验检测研究院, 国家市场监督管理总局重点实验室(调味品监管技术), 重庆 401121
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苏敏(1992—), 女, 硕士, 高级工程师, 主要研究方向为食品安全与检测。E-mail: 398568461@qq.com
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苏敏(1992—), 女, 硕士, 高级工程师, 主要研究方向为食品安全与检测。E-mail: 398568461@qq.com
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42(17): 285-293., articleTitle=Rapid screening and determination of 20 plant-derived toxins in honey by UPLC-Q-Exactive quadrupole-electrostatic field track trap high resolution mass spectrometry, refAbstract=null)], funds=[Fund(id=1177985603871326270, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, awardId=CQSJKJDW2023035, language=CN, fundingSource=重庆市市场监督管理局科研计划项目(CQSJKJDW2023035), fundOrder=null, country=null), Fund(id=1177985603955212352, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, awardId=CQSJKJDW2024037, language=CN, fundingSource=重庆市市场监督管理局科研计划项目(CQSJKJDW2024037), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1177985600423609313, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, xref=null, ext=[AuthorCompanyExt(id=1177985600431997922, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, companyId=1177985600423609313, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Chongqing Institute for Food and Drug Control, Key Laboratory of Condiment Supervision Technology, State Administration for Market Regulation, Chongqing 401121, China), AuthorCompanyExt(id=1177985600436192227, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, companyId=1177985600423609313, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=重庆市食品药品检验检测研究院, 国家市场监督管理总局重点实验室(调味品监管技术), 重庆 401121
)])], figs=[ArticleFig(id=1177985603133128750, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=EN, label=Fig.1, caption=
Structure diagram of mass spectrum, figureFileSmall=r0CjQx3rZtjehEOpXMPhng==, figureFileBig=Edny85LIeMfakmNe5DGG6A==, tableContent=null), ArticleFig(id=1177985603179266096, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=CN, label=图1, caption=
质谱结构图, figureFileSmall=r0CjQx3rZtjehEOpXMPhng==, figureFileBig=Edny85LIeMfakmNe5DGG6A==, tableContent=null), ArticleFig(id=1177985603229597746, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=EN, label=Table 1, caption=
Differences in Q-TOF and Q-orbitrap parameters
, figureFileSmall=null, figureFileBig=null, tableContent=
| 仪器 | 适宜质量范围 | 典型分辨率 | 扫描速率/Hz |
| Q-TOF | 高m/z | 15000~50000 | >20 |
| Q-Orbitrap | 低m/z | 35000~140000 | 1.5、3.0、6.0、12.0 |
), ArticleFig(id=1177985603305095220, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=CN, label=表1, caption=
Q-TOF与Q-Orbitrap参数区别
, figureFileSmall=null, figureFileBig=null, tableContent=
| 仪器 | 适宜质量范围 | 典型分辨率 | 扫描速率/Hz |
| Q-TOF | 高m/z | 15000~50000 | >20 |
| Q-Orbitrap | 低m/z | 35000~140000 | 1.5、3.0、6.0、12.0 |
), ArticleFig(id=1177985603388981302, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=EN, label=Table 2, caption=
Application of LC-HRMS to pesticide residues in food
, figureFileSmall=null, figureFileBig=null, tableContent=
| 序号 | 仪器 | 前处理 | 基质 | 检测项目 | 定量限/(μg/kg) | 文献 |
| 1 | UPLC-Q-Orbitrap | QuEChERS | 蔬菜 | 例行监测的61种农药 | 1~10 | [19] |
| 2 | UPLC-Q-Orbitrap | QuEChERS | 豆类杂粮 | 甲拌磷等53种农药 | 1~10 | [20] |
| 3 | UPLC-Q-Orbitrap | QuEChERS | 水产 | 甲胺磷等62种农药 | 2~10 | [21] |
| 4 | UPLC-Q-Orbitrap | 改良QuEChERS | 鸡蛋 | 除草剂、杀菌剂等5类94种农药 | 1~10 | [22] |
| 5 | UPLC-Q-Orbitrap | 改良QuEChERS | 鸡肉 | 双酚胺类和双酚肼类13种农药 | 0.2~1.0 | [23] |
| 6 | UPLC-Q-Orbitrap | 改良QuEChERS | 茶叶 | 166种农药 | 10~25 | [24] |
| 7 | UPLC-Q-Orbitrap | 改良QuEChERS | 蓝莓 | 亚砜磷等90种农药 | 0.2~20.2 | [25] |
| 8 | UPLC-Q-Orbitrap | QuEChERS | 猪肉、鸡肉 | 157种农药 | 0.2~8.8 | [26] |
| 9 | LC-Q-TOF | 改良QuEChERS | 芒果、香蕉、释迦
和莲雾 | 8种新烟碱类杀虫剂、25种杀菌剂33种农药 | 0.5~50 | [27] |
| 10 | LC-Q-Orbitrap | QuEChERS-冷冻诱导液液萃取 | 蔬菜、水果 | 77种农药 | 0.05~0.5 | [28] |
| 11 | LC-Q-TOF | 改良QuEChERS | 牛肉 | 129种农药残留 | 0.003~11.37 | [29] |
| 12 | UPLC-Q-Orbitrap | PRiME HLB | 杨梅 | 29种农药残留 | 6 | [30] |
| 13 | UPLC-Q-TOF | 改良QuEChERS | 蔬菜、水果 | 420种农药残留 | 0.1~5 | [31] |
| 14 | UPLC-Q-TOF | 改良QuEChERS | 青菜 | 214种农药残留 | 0.206~18.125 | [32] |
), ArticleFig(id=1177985603460284472, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=CN, label=表2, caption=
LC-HRMS在食品中农药残留的应用
, figureFileSmall=null, figureFileBig=null, tableContent=
| 序号 | 仪器 | 前处理 | 基质 | 检测项目 | 定量限/(μg/kg) | 文献 |
| 1 | UPLC-Q-Orbitrap | QuEChERS | 蔬菜 | 例行监测的61种农药 | 1~10 | [19] |
| 2 | UPLC-Q-Orbitrap | QuEChERS | 豆类杂粮 | 甲拌磷等53种农药 | 1~10 | [20] |
| 3 | UPLC-Q-Orbitrap | QuEChERS | 水产 | 甲胺磷等62种农药 | 2~10 | [21] |
| 4 | UPLC-Q-Orbitrap | 改良QuEChERS | 鸡蛋 | 除草剂、杀菌剂等5类94种农药 | 1~10 | [22] |
| 5 | UPLC-Q-Orbitrap | 改良QuEChERS | 鸡肉 | 双酚胺类和双酚肼类13种农药 | 0.2~1.0 | [23] |
| 6 | UPLC-Q-Orbitrap | 改良QuEChERS | 茶叶 | 166种农药 | 10~25 | [24] |
| 7 | UPLC-Q-Orbitrap | 改良QuEChERS | 蓝莓 | 亚砜磷等90种农药 | 0.2~20.2 | [25] |
| 8 | UPLC-Q-Orbitrap | QuEChERS | 猪肉、鸡肉 | 157种农药 | 0.2~8.8 | [26] |
| 9 | LC-Q-TOF | 改良QuEChERS | 芒果、香蕉、释迦
和莲雾 | 8种新烟碱类杀虫剂、25种杀菌剂33种农药 | 0.5~50 | [27] |
| 10 | LC-Q-Orbitrap | QuEChERS-冷冻诱导液液萃取 | 蔬菜、水果 | 77种农药 | 0.05~0.5 | [28] |
| 11 | LC-Q-TOF | 改良QuEChERS | 牛肉 | 129种农药残留 | 0.003~11.37 | [29] |
| 12 | UPLC-Q-Orbitrap | PRiME HLB | 杨梅 | 29种农药残留 | 6 | [30] |
| 13 | UPLC-Q-TOF | 改良QuEChERS | 蔬菜、水果 | 420种农药残留 | 0.1~5 | [31] |
| 14 | UPLC-Q-TOF | 改良QuEChERS | 青菜 | 214种农药残留 | 0.206~18.125 | [32] |
), ArticleFig(id=1177985603535781946, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=EN, label=Table 3, caption=
Application of LC-HRMS to veterinary drugs in food
, figureFileSmall=null, figureFileBig=null, tableContent=
| 序号 | 仪器 | 前处理 | 基质 | 检测项目 | 定量限/(μg/kg) | 文献 |
| 1 | LC-Q-Orbitrap | 提取: 80%乙腈-水溶液(含0.2%甲酸)
净化: Oasis PRiME HLB | 畜禽肉 | 108种兽药残留 | 0.2~100 | [39] |
| 2 | UPLC-Q-Orbitrap | 提取: 乙腈、乙酸乙酯
净化: PSA | 鱼 | 112种兽药残留 | 2.0 | [40] |
| 3 | UPLC-Q-TOF | 提取: 乙腈、0.1 mol/L EDTA | 牛奶 | 89种抗生素 | 0.1~9 | [41] |
| 4 | UPLC-Q-Orbitrap | 液液微萃取 | 养殖鱼 | 52种兽药残留 | 2~50 | [42] |
| 5 | LC-Q-Orbitrap | 提取: 80%乙腈-水溶液(含0.2%甲酸)
净化: Oasis PRiME HLB | 猪、鸡、鱼 | 14类160种兽药残留 | 0.5~50 | [43] |
| 6 | UPLC-Q-Orbitrap | 提取: 10 mmol/L EDTA-2Na溶液+乙腈:甲醇:水(3:1:1, 含1%乙酸)
净化: Oasis HLB | 鱼、虾、贝 | 11类79种兽药残留 | 0.2~5.0 | [44] |
| 7 | LC-Q-Orbitrap | 提取: 乙腈:水(8: 2)
净化: / | 水产品 | 15类123种兽药残留 | 0.1~50 | [45] |
| 8 | LC-Q-TOF | 提取: 甲醇, 0.1%甲酸, 0.1 mol/L EDTA
净化: / | 猪肉 | 65种兽药残留 | / | [46] |
| 9 | UPLC-Q-Orbitrap | 提取: 80%乙腈-水溶液(含0.2%甲酸)
净化: Oasis PRiME HLB | 动物源性食品 | 21类155种兽药残留 | 0.1~10 | [47] |
| 10 | UPLC-Q-TOF | 提取: 1%甲酸乙腈
净化: Oasis PRiME HLB | 猪肉、牛肉 | 30种兴奋剂类 | 0.2~4.0 | [48] |
| 11 | LC-Q-TOF | 提取: 乙腈、乙酸乙酯
净化: QuEChERS(增强型脂质吸附剂) | 牛肉、鸡肉、猪肝 | 32种激素类 | 3.0~30 | [49] |
| 12 | UPLC-Q-Orbitrap | 提取: 0.2%甲酸乙腈
净化: Oasis PRiME HLB | 乳制品 | 60种抗生素 | 5.0~10.0 | [50] |
| 13 | UPLC-Q-Orbitrap | 提取: 乙腈
净化: EMR-Lipid净化柱 | 猪肉 | 阿托品、普鲁卡因 | 0.10 | [51] |
| 14 | UPLC-Q-Orbitrap | 提取: 乙腈
净化: 正己烷 | 猪肉 | 9种大环内酯类 | 0.1~0.4 | [52] |
| 15 | UPLC-Q-Orbitrap | 提取: 1%甲酸乙腈
净化: BONDESIL-SAX、C18 | 鸡蛋 | 磺胺喹恶啉、二甲氧苄啶 | 0.3~0.6 | [53] |
), ArticleFig(id=1177985603598696508, tenantId=1146029695717560320, journalId=1149652044408987649, articleId=1153986582030836387, language=CN, label=表3, caption=
LC-HRMS在食品中兽药残留的应用
, figureFileSmall=null, figureFileBig=null, tableContent=
| 序号 | 仪器 | 前处理 | 基质 | 检测项目 | 定量限/(μg/kg) | 文献 |
| 1 | LC-Q-Orbitrap | 提取: 80%乙腈-水溶液(含0.2%甲酸)
净化: Oasis PRiME HLB | 畜禽肉 | 108种兽药残留 | 0.2~100 | [39] |
| 2 | UPLC-Q-Orbitrap | 提取: 乙腈、乙酸乙酯
净化: PSA | 鱼 | 112种兽药残留 | 2.0 | [40] |
| 3 | UPLC-Q-TOF | 提取: 乙腈、0.1 mol/L EDTA | 牛奶 | 89种抗生素 | 0.1~9 | [41] |
| 4 | UPLC-Q-Orbitrap | 液液微萃取 | 养殖鱼 | 52种兽药残留 | 2~50 | [42] |
| 5 | LC-Q-Orbitrap | 提取: 80%乙腈-水溶液(含0.2%甲酸)
净化: Oasis PRiME HLB | 猪、鸡、鱼 | 14类160种兽药残留 | 0.5~50 | [43] |
| 6 | UPLC-Q-Orbitrap | 提取: 10 mmol/L EDTA-2Na溶液+乙腈:甲醇:水(3:1:1, 含1%乙酸)
净化: Oasis HLB | 鱼、虾、贝 | 11类79种兽药残留 | 0.2~5.0 | [44] |
| 7 | LC-Q-Orbitrap | 提取: 乙腈:水(8: 2)
净化: / | 水产品 | 15类123种兽药残留 | 0.1~50 | [45] |
| 8 | LC-Q-TOF | 提取: 甲醇, 0.1%甲酸, 0.1 mol/L EDTA
净化: / | 猪肉 | 65种兽药残留 | / | [46] |
| 9 | UPLC-Q-Orbitrap | 提取: 80%乙腈-水溶液(含0.2%甲酸)
净化: Oasis PRiME HLB | 动物源性食品 | 21类155种兽药残留 | 0.1~10 | [47] |
| 10 | UPLC-Q-TOF | 提取: 1%甲酸乙腈
净化: Oasis PRiME HLB | 猪肉、牛肉 | 30种兴奋剂类 | 0.2~4.0 | [48] |
| 11 | LC-Q-TOF | 提取: 乙腈、乙酸乙酯
净化: QuEChERS(增强型脂质吸附剂) | 牛肉、鸡肉、猪肝 | 32种激素类 | 3.0~30 | [49] |
| 12 | UPLC-Q-Orbitrap | 提取: 0.2%甲酸乙腈
净化: Oasis PRiME HLB | 乳制品 | 60种抗生素 | 5.0~10.0 | [50] |
| 13 | UPLC-Q-Orbitrap | 提取: 乙腈
净化: EMR-Lipid净化柱 | 猪肉 | 阿托品、普鲁卡因 | 0.10 | [51] |
| 14 | UPLC-Q-Orbitrap | 提取: 乙腈
净化: 正己烷 | 猪肉 | 9种大环内酯类 | 0.1~0.4 | [52] |
| 15 | UPLC-Q-Orbitrap | 提取: 1%甲酸乙腈
净化: BONDESIL-SAX、C18 | 鸡蛋 | 磺胺喹恶啉、二甲氧苄啶 | 0.3~0.6 | [53] |
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