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Self-designed experiment in instrumental analysis: online solid phase extraction-high performance liquid chromatography- post column derivatization for the determination of trace pesticide residues in vegetables
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Yan LI, Yuntao LIU, Xien HUANG, Ziyi ZHOU, Ziqiu WANG, Jing WANG, Fei DING, Jing NAN, Anna TANG*
Chinese Journal of Chromatography | 2026, 44(9) : 1074 - 1081
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Chinese Journal of Chromatography | 2026, 44(9): 1074-1081
• Teaching Research •
Self-designed experiment in instrumental analysis: online solid phase extraction-high performance liquid chromatography- post column derivatization for the determination of trace pesticide residues in vegetables
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Yan LI, Yuntao LIU, Xien HUANG, Ziyi ZHOU, Ziqiu WANG, Jing WANG, Fei DING, Jing NAN, Anna TANG*
Affiliations
  • 1College of Chemistry, Nankai University, National Demonstration Center for Experimental Chemistry Education, Tianjin 300071, China
Published: 2026-09-08 doi: 10.3724/SP.J.1123.2025.10020
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In line with modern educational concepts and the demand for innovative talents, a course focused on self-designed experiments has been incorporated into the traditional instrumental analysis curriculum. This initiative aims to enhance students’ ability to apply knowledge effectively. While mastering instrument operation, students are encouraged to anticipate potential challenges during experiments. Under instructor guidance, students constructed an online system for solid-phase extraction coupled with liquid chromatography and post-column derivatization with fluorescence detection. They addressed real analytical needs such as improving sensitivity and precision, minimizing interference, and conducting rapid analyses of trace carbamate pesticides in vegetable samples. This self-designed liquid chromatography experiment course, tailored to students’ learning characteristics and interests, successfully engages their initiative and fosters independent innovation capabilities. As a result, it also improves teaching effectiveness and enhances learning efficiency.

self-designed experiment  /  online solid-phase extraction (online SPE)  /  high performance liquid chromatography (HPLC)  /  post-column derivatization  /  pesticide residue analysis
李琰, 刘韫滔, 黄熙恩, 周子易, 王梓丘, 王京, 丁飞, 南晶, 唐安娜. 仪器分析自主实验设计:在线固相萃取-高效液相色谱-柱后衍生测定蔬菜中痕量农药残留. 色谱, 2026 , 44 (9) : 1074 -1081 . DOI: 10.3724/SP.J.1123.2025.10020
Yan LI, Yuntao LIU, Xien HUANG, Ziyi ZHOU, Ziqiu WANG, Jing WANG, Fei DING, Jing NAN, Anna TANG. Self-designed experiment in instrumental analysis: online solid phase extraction-high performance liquid chromatography- post column derivatization for the determination of trace pesticide residues in vegetables[J]. Chinese Journal of Chromatography, 2026 , 44 (9) : 1074 -1081 . DOI: 10.3724/SP.J.1123.2025.10020
Year 2026 volume 44 Issue 9
68
32
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doi: 10.3724/SP.J.1123.2025.10020
  • Receive Date:2025-10-28
  • Online Date:2026-10-27
  • Published:2026-09-08
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  • Received:2025-10-28
Affiliations
    1College of Chemistry, Nankai University, National Demonstration Center for Experimental Chemistry Education, Tianjin 300071, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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