Under new pharmaceutical science initiatives and innovation training, we address complex biological sample analysis. A comprehensive pharmaceutical experiment was designed for this purpose. It integrates pharmaceutical analysis, polymer chemistry, and analytical chemistry. Advanced molecular imprinting technology (MIT) serves as the key tool. Cholic acid, a significant endogenous metabolite, is selected as the template. Cholic acid molecularly imprinted polymers (CA-MIPs) are synthesized via precipitation polymerization. Their structure is characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). The imprinting site and formation mechanism is elucidated. Adsorption performance is evaluated by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Selectivity and enrichment efficiency in plasma are assessed. The experiment covers material design, synthesis, and characterization. It also includes application performance evaluation. This demonstrates high-level innovation and challenge. The practice enhances students’ innovative thinking and practical skills. It fosters social and professional responsibility. The project cultivates high-quality applied pharmaceutical talents. This represents a beneficial practice in holistic education.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |