Full
Outline
Copeptin， as a stable surrogate biomarker for arginine vasopressin （AVP）， plays an important role in the differential diagnosis of polyuria-polydipsia syndromes. Current guidelines recommend dynamic monitoring of copeptin levels during stimulation tests to assist in differentiating AVP deficiency （AVP-D） from primary polydipsia. Although these methods are well-established， they have certain limitations. Therefore， safer and more feasible stimulants with reliable effects are of clinical interest. Additionally， the currently available copeptin assay， which is based on time-resolved immunofluorescence assay， can be subject to interference from autoantibodies or hemolysis. This study aims to evaluate the effects of four common stimulants used in growth hormone function tests， namely levodopa， insulin， glucagon， and octreotide， on copeptin levels using a reliable liquid chromatography-tandem mass spectrometry （LC-MS/MS） method developed in our laboratory. A total of 62 subjects undergoing growth hormone function tests were retrospectively enrolled and stratified by stimulation type： levodopa （n=28）， insulin-induced hypoglycemia （n=7）， glucagon （n=20）， and octreotide （n=7）. Blood samples were collected at baseline， 30， 60， 90， and 120 min （for the glucagon stimulation test， samples were collected at 120 and 180 min） for growth hormone determination. Copeptin levels at each time point were measured using LC-MS/MS. The effects of stimulation and correlations were analyzed using the Wilcoxon paired signed-rank test， Mann-Whitney test， and Spearman correlation analysis. The results demonstrated that copeptin levels increased under levodopa stimulation by a maximum of 8.47-fold of baseline （p<0.000 1）， under insulin stimulation by a maximum of 5.85-fold of baseline （p=0.031 2）， under glucagon stimulation by a maximum of 1.43-fold of baseline （p<0.000 1）， and decreased under octreotide to 43% of baseline （p<0.05）. No significant correlation was observed between copeptin level changes and those in growth hormone levels. In the levodopa-stimulation group， the maximum value of copeptin in patients with AVP deficiency was significantly lower than that in non-AVP deficiency patients （p=0.000 2）， and the area under the receiver operating characteristic curve was 0.98 （95% confidence interval 0.94–1.00， p=0.002 1）. Our results demonstrate that levodopa and insulin can effectively stimulate copeptin secretion， whereas octreotide exhibits a suppressive effect. These findings offer important physiological insights into AVP regulation and indicate that certain GH stimulation agents may have extended utility in copeptin-based diagnostic strategies. The LC-MS/MS method for detecting copeptin has potential clinical value in the diagnosis of patients with AVP deficiency and can provide a new detection method for clinical practice. However， the relatively small sample sizes restrict the statistical power， and larger prospective studies are warranted.
45
21
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
