Instrumental analysis is a basic undergraduate course. It is mainly teaching-oriented for chemistry， materials， biology， environment， food and other majors. Among them， chromatography and spectrometry are two of the key teaching contents in the course of instrumental analysis. They have mature theoretical teaching systems and the supporting teaching experiments are relatively coincident. However， the main knowledge points are cluttered and complicated， and the connections between these chapters are not close enough. It’s hard for students to expand and comprehensively apply knowledge on the basis of theoretical knowledge and experimental technology. Besides， the innovation and cutting-edge aspects are relatively lacking. Based on the geographical advantages of coastal schools， this paper combined with the cases of new pollutants microplastics to explore the integrated teaching method of chromatography and spectroscopy in instrumental analysis. It systematically expands the teaching content， teaching mode and teaching effect respectively. The teaching contents of chromatographic and spectroscopic principles are expanded. The analysis abilities were demonstrated by case teaching methods and comparative teaching method. The integrated method of chromatography and spectroscopy can combine the characteristics to achieve the effective complementarity of the two methods. It combines the fast high-throughput analysis abilities of chromatography and the high selectivity and sensitivity of spectroscopy. This course is conducive to promoting the standardization and normalization of screening and identification methods for new pollutants. It can provide more advanced analytical techniques for monitoring new pollutants. In addition， this course can introduce hot topics to broaden the scope of subject knowledge. It is helpful to improve students’ ability to investigate relevant literature， understand scientific frontier development of science， and master the comprehensive application of instruments. It is also beneficial to guide students to master the induction， comparison and analysis of basic theoretical knowledge， which is conducive to students’ integration of instrument analysis. At the same time， it helps students grasp the bottleneck problems in the process of the instrument development. It then motivates students to become leading talents to meet the major strategic needs of the country. This paper is in favor of cultivating students’ innovative awareness and scientific spirit， and finally realizing the fundamental task of establishing morality and cultivating talents.

chromatography  /  spectroscopy  /  integrated instruction  /  new marine pollutants  /  microplastics