Ionogels have garnered great attention as promising soft conducting materials for the fabrication of flexible energy storage devices, soft actuators, and ionotronics. However, the leakage of the ionic liquids, weak mechanical strength, and poor manufacturability have greatly limited their reliability and applications. Here, we propose a new ionogel synthesis strategy by utilizing granular zwitterionic microparticles to stabilize ionic liquids. The ionic liquids swell the microparticles and physically crosslink microparticles via either electronic interaction or hydrogen bonding. Further introducing a photocurable acrylic monomer enables the fabrication of double-network (DN) ionogels with high stretchability (>600%) and ultrahigh toughness (fracture energy > 10 kJ/m²). The synthesized ionogels exhibit a wide working temperature of −60 to 90 °C. By tuning the crosslinking density of microparticles and physical crosslinking strength of ionogels, we synthesize DN ionogel inks and print them into three-dimensional (3D) motifs. Several ionogel-based ionotronics are 3D printed as demonstrations, including strain gauges, humidity sensors, and ionic skins made of capacitive touch sensor arrays. Via covalently linking ionogels with silicone elastomers, we integrate the ionogel sensors onto pneumatic soft actuators and demonstrate their capacities in sensing large deformation. As our last demonstration, multimaterial direct ink writing is harnessed to fabricate highly stretchable and durable alternating-current electroluminescent devices with arbitrary structures. Our printable granular ionogel ink represents a versatile platform for the future manufacturing of ionotronics.

Cell replacement therapy using neural progenitor cells (NPCs) has been shown to be an effective treatment for ischemic stroke. However, the therapeutic effect is unsatisfactory due to the imbalanced homeostasis of the local microenvironment after ischemia. Microenvironmental acidosis is a common imbalanced homeostasis in the penumbra and could activate acid-sensing ion channels 1a (ASIC1a), a subunit of proton-gated cation channels following ischemic stroke. However, the role of ASIC1a in NPCs post-ischemia remains elusive. Here, our results indicated that ASIC1a was expressed in NPCs with channel functionality, which could be activated by extracellular acidification. Further evidence revealed that ASIC1a activation inhibited NPC migration and neurogenesis through RhoA signaling-mediated reorganization of filopodia formation, which could be primarily reversed by pharmacological or genetic disruption of ASIC1a. In vivo data showed that the knockout of the ASIC1a gene facilitated NPC migration and neurogenesis in the penumbra to improve behavioral recovery after stroke. Subsequently, ASIC1a gain of function partially abrogated this effect. Moreover, the administration of ASIC1a antagonists (amiloride or Psalmotoxin 1) promoted functional recovery by enhancing NPC migration and neurogenesis. Together, these results demonstrate targeting ASIC1a is a novel strategy potentiating NPC migration toward penumbra to repair lesions following ischemic stroke and even for other neurological diseases with the presence of niche acidosis.

Temperature sensing is of high value in the wearable healthcare, robotics/prosthesis, and noncontact physiological monitoring. However, the common mechanic deformation, including pressing, bending, and stretching, usually causes undesirable feature size changes to the inner conductive network distribution of temperature sensors, which seriously influences the accuracy. Here, inspired by the transient receptor potential mechanism of biological thermoreceptors that could work precisely under various skin contortions, we propose an MXene/Clay/poly(N-isopropylacrylamide) (PNIPAM) (MCP) hydrogel with high stretchability, spike response, and deformation insensitivity. The dynamic spike response is triggered by the inner conductive network transformation from the 3-dimensional structure to the 2-dimensional surface after water being discharged at the threshold temperature. The water discharge is solely determined by the thermosensitivity of PNIPAM, which is free from mechanical deformation, so the MCP hydrogels can perform precise threshold temperature (32 °C) sensing under various deformation conditions, i.e., pressing and 15% stretching. As a proof of concept, we demonstrated the applications in plant electronics for the real-time surface temperature monitoring and skin electronics for communicating between human and machines. Our research opens venues for the accurate temperature-threshold sensation on the complicated surface and mechanical conditions.

Conventionally, to produce a linear motion, one motor’s stator is employed to drive one runner moving forward or backward. So far, there is almost no report of one electromechanical motor or piezoelectric ultrasonic motor that can directly generate two symmetrical linear motions, while this function is desired for precise scissoring and grasping in the minimally invasive surgery field. Herein, we report a brand-new symmetric-actuating linear piezoceramic ultrasonic motor capable of generating symmetrical linear motions of two outputs directly without additional mechanical transmission mechanisms. The key component of the motor is an (2 × 3) arrayed piezoceramic bar stator operating in the coupled resonant mode of the first longitudinal (L₁) and third bending (B₃) modes, leading to symmetric elliptical vibration trajectories at its two ends. A pair of microsurgical scissors is used as the end-effector, demonstrating a very promising future for high-precision microsurgical operations. The sliders of the prototype show the following features: (a) symmetrical, fast relative moving velocity (~1 m/s) outward or inward simultaneously; (b) high step resolution (40 nm); and (c) high power density (405.4 mW/cm³) and high efficiency (22.1%) that are double those of typical piezoceramic ultrasonic motors, indicating the full capacity of symmetric-actuating linear piezoceramic ultrasonic motor working in symmetric operation principle. This work also has enlightening significance for future symmetric-actuating device designs.

Flexible full-textile pressure sensor is able to integrate with clothing directly, which has drawn extensive attention from scholars recently. But the realization of flexible full-textile pressure sensor with high sensitivity, wide detection range, and long working life remains challenge. Complex recognition tasks necessitate intricate sensor arrays that require extensive data processing and are susceptible to damage. The human skin is capable of interpreting tactile signals, such as sliding, by encoding pressure changes and performing complex perceptual tasks. Inspired by the skin, we have developed a simple dip-and-dry approach to fabricate a full-textile pressure sensor with signal transmission layers, protective layers, and sensing layers. The sensor achieves high sensitivity (2.16 kPa⁻¹), ultrawide detection range (0 to 155.485 kPa), impressive mechanical stability of 1 million loading/unloading cycles without fatigue, and low material cost. The signal transmission layers that collect local signals enable real-world complicated task recognition through one single sensor. We developed an artificial Internet of Things system utilizing a single sensor, which successfully achieved high accuracy in 4 tasks, including handwriting digit recognition and human activity recognition. The results demonstrate that skin-inspired full-textile sensor paves a promising route toward the development of electronic textiles with important potential in real-world applications, including human–machine interaction and human activity detection.

Neuromuscular dysfunction is tightly associated with muscle wasting that occurs with age or due to degenerative diseases. However, the molecular mechanisms underlying neuromuscular dysfunction are currently unclear. Recent studies have proposed important roles of Protein arginine methyltransferase 1 (Prmt1) in muscle stem cell function and muscle maintenance. In the current study, we set out to determine the role of Prmt1 in neuromuscular function by generating mice with motor neuron-specific ablation of Prmt1 (mnKO) using Hb9-Cre. mnKO exhibited age-related motor neuron degeneration and neuromuscular dysfunction leading to premature muscle loss and lethality. Prmt1 deficiency also impaired motor function recovery and muscle reinnervation after sciatic nerve injury. The transcriptome analysis of aged mnKO lumbar spinal cords revealed alterations in genes related to inflammation, cell death, oxidative stress, and mitochondria. Consistently, mnKO lumbar spinal cords of sciatic nerve injury model or aged mice exhibited elevated cellular stress response in motor neurons. Furthermore, Prmt1 inhibition in motor neurons elicited mitochondrial dysfunction. Our findings demonstrate that Prmt1 ablation in motor neurons causes age-related motor neuron degeneration attributing to muscle loss. Thus, Prmt1 is a potential target for the prevention or intervention of sarcopenia and neuromuscular dysfunction related to aging.

Sustained wear damages on the sliding surfaces of alloys are generally the culprit responsible for the failure of various mechanical systems. Inspired by high-entropy effects, here we deliberately deploy nanohierarchical architecture with composition undulation in a Ni₅₀(AlNbTiV)₅₀ complex concentrated alloy, which yields ultralow wear rate within the order of 10⁻⁷ to 10⁻⁶ mm³/Nm between room temperature and 800 °C. Such remarkable wear resistance heretofore represents one of the highest wear resistance reported for the bulk alloys or composites, and originates from the multi-type adaptive friction interface protection governed by intrinsically nano-coupled grains and nanoprecipitates. This cooperative heterostructure releases gradient frictional stress in stages upon wear at room temperature through the coexistence of multiple deformation pathways while activating a dense nanocrystalline glaze layer upon wear at 800 °C to minimize adhesive and oxidative wear. Our work uncovers a practical avenue for tailoring wear properties with multicomponent heterostructures over a wide temperature range.

The biomimetic construction of a microstructural–mechanical–electrical anisotropic microenvironment adaptive to the native cardiac tissue is essential to repair myocardial infarction (MI). Inspired by the 3D anisotropic characteristic of the natural fish swim bladder (FSB), a novel flexible, anisotropic, and conductive hydrogel was developed for tissue-specific adaptation to the anisotropic structural, conductive, and mechanical features of the native cardiac extracellular matrix. The results revealed that the originally stiff, homogeneous FSB film was tailored to a highly flexible anisotropic hydrogel, enabling its potential as a functional engineered cardiac patch (ECP). In vitro and in vivo experiments demonstrated the enhanced electrophysiological activity, maturation, elongation, and orientation of cardiomyocytes (CMs), and marked MI repair performance with reduced CM apoptosis and myocardial fibrosis, thereby promoting cell retention, myogenesis, and vascularization, as well as improving electrical integration. Our findings offer a potential strategy for functional ECP and provides a novel strategy to bionically simulate the complex cardiac repair environment.

Spermatogonial stem cells (SSCs) have important applications in both reproduction and regenerative medicine. Nevertheless, specific genes and signaling transduction pathways in mediating fate decisions of human SSCs remain elusive. Here, we have demonstrated for the first time that OIP5 (Opa interacting protein 5) controlled the self-renewal and apoptosis of human SSCs. RNA sequencing identified that NCK2 was a target for OIP5 in human SSCs, and interestingly, OIP5 could interact with NCK2 as shown by Co-IP (co-immunoprecipitation), IP-MS (mass spectrometry), and GST pulldown assays. NCK2 silencing decreased human SSC proliferation and DNA synthesis but enhanced their apoptosis. Notably, NCK2 knockdown reversed the influence of OIP5 overexpression on human SSCs. Moreover, OIP5 inhibition decreased the numbers of human SSCs at S and G2/M phases, while the levels of numerous cell cycle proteins, including cyclins A2, B1, D1, E1 and H, especially cyclin D1, were remarkably reduced. Significantly, whole-exome sequencing of 777 patients with nonobstructive azoospermia (NOA) revealed 54 single-nucleotide polymorphism mutations of the OIP5 gene (6.95%), while the level of OIP5 protein was obviously lower in testes of NOA patients compared to fertile men. Collectively, these results implicate that OIP5 interacts with NCK2 to modulate human SSC self-renewal and apoptosis via cell cyclins and cell cycle progression and that its mutation and/or lower expression is correlated with azoospermia. As such, this study offers novel insights into molecular mechanisms underlying the fate determinations of human SSCs and the pathogenesis of NOA, and it provides new targets for treating male infertility.

So far, there have been 4 methods to control chirality including the use of chiral auxiliaries, reagents, solvents, and catalysts documented in literature and textbooks. Among them, asymmetric catalysts are normally divided into homogeneous and heterogeneous catalysis. In this report, we present a new type of asymmetric control—asymmetric catalysis via chiral aggregates that would not belong to the above categories. This new strategy is represented by catalytic asymmetric dihydroxylation reaction of olefins in which chiral ligands are aggregated by taking advantage of typical aggregation-induced emission systems containing tetrahydrofuran and H₂O cosolvents. It was proven that the chiral induction can be enhanced from er of 78:22 to 97:3 simply by changing the ratios of these 2 cosolvents. The formation of chiral aggregates of asymmetric dihydroxylation ligands, (DHQD)₂PHAL and (DHQ)₂PHAL, has been proven by aggregation-induced emission and a new analytical tool—aggregation-induced polarization established by our laboratory. In the meanwhile, chiral aggregates were found to be formed either by adding NaCl into tetrahydrofuran/H₂O systems or by increasing concentrations of chiral ligands. The present strategy also showed promising reverse control of enantioselectivity in the Diels–Alder reaction. This work is anticipated to be extended broadly to general catalysis, especially to asymmetric catalysis in the future.