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Coumarin Glycosides Reverse Enterococci-Facilitated Enteric Infections
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Wenjiao Xu1, 2, Guixin Yuan3, Yuwen Fang1, 2, Xiaojia Liu1, 2, Xiaowei Ma1, 2, Kui Zhu1, 2, *
Research. Vol 7 Article ID 0374
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Research. Vol 7 Article ID 0374
• Research Article •
Coumarin Glycosides Reverse Enterococci-Facilitated Enteric Infections
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Wenjiao Xu1, 2, Guixin Yuan3, Yuwen Fang1, 2, Xiaojia Liu1, 2, Xiaowei Ma1, 2, Kui Zhu1, 2, *
Affiliations
  • 1National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • 2Engineering Research Center of Animal Innovative Drugs and Safety Evaluation, Ministry of Education, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • 3Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing 100193, China.
Published: 2024-05-16 doi: 10.34133/research.0374
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Commensal enterococci with pathogenic potential often facilitate the growth of diverse pathogens, thereby exacerbating infections. However, there are few effective therapeutic strategies to prevent and intervene in enterococci-mediated polymicrobial infections. Here, we find that enterococci at high density drive the expansion and pathogenicity of enteric Salmonella enterica serotype Typhimurium (S. Tm). Subsequently, we show that the driving role of enterococci in such infections is counteracted by dietary coumarin glycosides in vivo. Enterococci, which are tolerant of iron-deficient environments, produce β-glucosidases to hydrolyze coumarin glycosides into bioactive aglycones, inhibiting S. Tm growth and ameliorating the severity of S. Tm-induced symptoms by inducing iron limitation. Overall, we demonstrate that coumarin glycosides as a common diet effectively reverse enterococci-facilitated enteric infections, providing an alternative intervention to combat polymicrobial infections.

Wenjiao Xu, Guixin Yuan, Yuwen Fang, Xiaojia Liu, Xiaowei Ma, Kui Zhu. Coumarin Glycosides Reverse Enterococci-Facilitated Enteric Infections[J]. Research, 2024 , 7 (5) : 0374 . DOI: 10.34133/research.0374
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Introduction
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Enterococci are commensals of the gut microbiota in both animals and humans, causing latent risks under specific conditions and therefore considered as “pathobionts”. Many factors can favor the overgrowth of enterococci in the gut, including medical reasons, dietary habits, and susceptible individuals [1]. Enterococcal intestinal domination, especially the devastating dissemination of vancomycin-resistant enterococci, has been identified as an indicator of subsequent infections and adverse outcomes [2,3]. Numerous studies demonstrated that enterococci facilitate the growth and pathogenicity of various pathogens, such as Clostridioides difficile, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Gardnerella vaginalis, and Candida albicans, via up-regulation of virulence, metabolic cross-feeding, biofilm formation, immune evasion, and transmission of drug resistance [4–9]. For instance, enterococci can promote toxin production of C. difficile through arginine catabolism in the gut, enhancing the fitness of C. difficile [10]. The driving role of enterococci in polymicrobial infection contributes to recurrent infections with elevated rates of treatment failure [11].
Given that multidrug-resistant enterococci are prone to overgrow in the gut after antibiotic treatments [12,13], enterococci-associated polymicrobial infections are difficult to eradicate by routine antibiotics. Seriously, the ability of enterococci to promote the proliferation, pathogenesis, and persistence of various pathogens further compromises the efficacy of existing antibiotics [14,15]. As an example, Enterococcus faecalis fuels the rapid autoaggregation and growth of E. coli, thus reinforcing dual-species biofilms to withstand harsh stresses including antibiotic treatments [16]. Consequently, exploring effective therapeutic strategies of enterococci-associated polymicrobial infections is an emergent and challenging problem to be solved.
Mounting evidence suggests that dietary plants offer a promising approach to improving host health and preventing diseases [17]. Natural coumarins, ubiquitously distributed in plant-based foods and medicinal herbs, possess the advances in structural diversity, accessibility, and robust antimicrobial activity, with an average intake of 1.2 mg/d for a 60-kg consumer [18,19]. However, many natural products, including coumarins, are often glycoconjugated as glycosides to enable storage and solubility in plants, exhibiting less or no antimicrobial activity compared with the deglycosylated counterparts (aglycones) [20,21]. Therefore, the deglycosylation of coumarin glycosides is the limiting step to modulate their bioavailability and bioactivities in hosts [22]. Although pioneering studies have reported that dietary glycosides can be converted into bioactive aglycones by gut microorganisms [23], the specific bacteria and proteins involved in coumarin glycoside utilization remain poorly understood.
In this work, we investigated the hydrolytic capacity of enterococci to coumarin glycosides, offering an effective approach to combating enterococci-mediated polymicrobial infections. First, we show the bacterial cooperation that enterococci promote the expansion and pathogenicity of enteric Salmonella enterica serotype Typhimurium (S. Tm). Next, our findings illustrate that the oral administration of coumarin glycosides reverses the promotion of enterococci to S. Tm infection. Specifically, enterococci-derived β-glucosidases (BGLs) hydrolyze coumarin glycosides into antibacterial aglycones, inhibiting the growth of S. Tm by outcompeting for iron. The interventions of coumarin glycosides in enterococci-mediated polymicrobial infection are validated in both in vitro and animal models, suggesting that diet-based strategies are encouraging approaches to mitigating enteric polymicrobial infections.
Results
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Enterococci promote the expansion and pathogenicity of S. Tm
To identify the driving impact of enterococci on polymicrobial infections in the gut, we used Enterococcus faecium and enteropathogenic S. Tm as a model. First, we quantified their interactions by assessing the growth dynamics of S. Tm 15E475 in cocultures with E. faecium CAU369 at inoculum ratios of 1:1, 1:10, and 1:100 (S. Tm: E. faecium), respectively. The growth of S. Tm in the cocultures presented an identical pattern to that of monocultures (Fig. S1A), indicating that E. faecium does not affect the growth of S. Tm under planktonic cultures. Given that enterococci-associated biofilm formation frequently enhances the proliferation of other pathogens, we determined whether the interaction between E. faecium CAU369 and S. Tm 15E475 affects biofilm biomass based on the standard crystal violet assay. We found that the cocultures showed significantly higher biofilm formation than monocultures at 72 and 96 h (Fig. S1B), suggesting that the promotion of enterococci to S. Tm growth is dependent on local bacterial communication.
To better mimic the environment of polymicrobial interactions, we used macrocolonies as a surrogate for biofilm formation tests to determine how enterococci modulate the growth of S. Tm. We grew the cocultured macrocolonies at various inoculum ratios of 1:1, 1:10, and 1:100 (S. Tm: E. faecium), maintaining the initial number of S. Tm constant. In comparison with macrocolonies of single species, we observed augmented and specific accumulation of biomass in cocultured macrocolonies (Fig. 1A). The higher ratio of E. faecium to S. Tm (from 1:1 to 1:100) potentiated the production of biofilm biomass in macrocolonies (Fig. 1B), indicating by the bacterial load that the presence of enterococci resulted in significantly more S. Tm number in cocultured macrocolonies (P = 0.022). Conversely, the number of E. faecium remained similar between single-and dual-species macrocolonies (P = 0.431), suggesting that E. faecium is still growing well when the cocultured S. Tm growth is robust. However, we did not observe a similar phenomenon in other dual-species macrocolonies, including methicillin-resistant S. aureus T144, E. coli B2 and P. aeruginosa PAO1 in the presence of E. faecium CAU369 (Fig. S1C). Consequently, we focused on the high inoculum ratio of E. faecium to S. Tm for subsequent studies.
Next, we investigated the impact of E. faecium on the virulence of invading S. Tm. Our findings revealed that high-density enterococci significantly up-regulated the relative expression of virulence genes (VGs) associated with T3SS-1 of S. Tm (Fig. S1D), which located in Salmonella pathogenicity islands-1, facilitating rapid replication of S. Tm in the gut [24,25]. Overall, these results indicate that E. faecium enhances the growth and virulence of S. Tm within biofilms (Fig. 1C). To evaluate the effect of E. faecium on S. Tm infection in vivo, we established a coinfected mouse model (Fig. 1D). Given that enterococcal overgrowth in the gut frequently serves as a precursor to subsequent infections of other pathogens [2], we gave mice an oral administration of E. faecium CAU369 24 h in advance to establish an ecological niche dominated by enterococci prior to S. Tm infection (Fig. S2A). Notably, mice coinfected with E. faecium showed higher burdens of S. Tm in cecal contents and livers compared with those infected with S. Tm alone (Fig. 1E). Meanwhile, coinfected mice showed less food intake and more weight loss (Fig. 1F and Fig. S2B). The intestines and livers of coinfected mice exhibited more severe injuries, as indicated by greater shrinkage and hemorrhage of the large intestine (Fig. 1G); a more swollen, yellowish, and brittle phenotype and increased organ indices of the liver (Fig. S2C and Fig. 1H); more integrity loss and inflammatory cell infiltration in the cecum, colon, and liver (Fig. 1I and Fig. S2D); and significantly up-regulated levels of inflammatory factor in the cecum and liver (Fig. 1J and Fig. S2E). Taken together, these findings demonstrate the successful establishment of a model wherein enterococci drive the growth of enteropathogenic bacteria, laying the groundwork for further exploration of therapeutic strategies.
Coumarin glucosides inhibit S. Tm growth in the presence of enterococci
Coumarins, in the form of aglycones or glycosides, are abundantly present in the human diet [19], with their bioavailability dependent on structures and stability. Aglycones can be readily absorbed in the small intestine, whereas glycosides exhibit very low absorptivity and primarily transit to the large intestine [26,27]. Therefore, coumarin glycosides have the robust advantage of oral administration, allowing them to reach infection sites of enteropathogenic bacteria. To screen representative coumarin compounds, we collected 30 coumarins and their analogs from dietary and medicinal plants and assessed their antibacterial activities (Fig. 2A). Fraxetin (FXE) showed the predominant antibacterial activity (Table S1). Therefore, we selected FXE and its glycoside, fraxin (FX), as the representative aglycone and glycoside, respectively, for following mechanistic studies.
To define the role of enterococci in the biological activity of coumarin glycosides, we examined the growth kinetics of S. Tm in cocultures with E. faecium plus FX. Intriguingly, the presence of E. faecium with FX markedly inhibited the S. Tm growth, whereas the single addition of E. faecium or FX has no antibacterial activity (Fig. 2B and Fig. S3), implying that E. faecium may hydrolyze inactive FX into bioactive FXE. To ascertain the generality of this phenomenon among E. faecium isolates and other enterococcal species, we tested the S. Tm number in cocultures with diverse enterococcal isolates plus FX. All enterococcal isolates displayed similar activities that their presence with FX inhibited the growth of S. Tm (Fig. 2C and Fig. S4A). To directly verify the hydrolytic capacity of enterococci to FX, we constructed the tandem mass spectrometry (MS/MS) spectrum of FXE (Fig. 2D) and measured the concentrations of FXE in cocultured supernatants of FX with diverse enterococcal species using liquid chromatography with MS/MS (LC-MS/MS) analysis. As anticipated, enterococci efficiently hydrolyzed FX into FXE, with an average hydrolytic rate exceeding 54% for all assayed enterococcal isolates (Fig. 2E). The glycosidic bond of FX is located at the C-8 position. To evaluate the diversity of substrates catalyzed by enterococci, we assessed the hydrolytic activity of enterococci on esculin and daphnin, whose glycosidic bonds are at positions C-6 and C-7, respectively. Consistently, enterococcal isolates are capable of hydrolyzing esculin and daphnin as well, with average hydrolysis rates of 57.47% and 25.35%, respectively (Fig. 2F and G and Fig. S4B). Altogether, these findings denote that enterococci hydrolyze coumarin glycosides into antibacterial aglycones (Fig. 2H), thereby inhibiting the growth of S. Tm.
Enterococci-derived BGLs hydrolyze coumarin glycosides into antibacterial aglycones
Considering that plant-derived BGLs are responsible for the hydrolysis of coumarin glycosides [28], we hypothesized that enterococci hydrolyze coumarin glycosides by secreting similar BGLs. First, we screened the bgl gene encoding BGLs in enterococcal isolates based on whole-genome sequencing. As expected, E. faecium isolates, including E. faecium CAU369, carry homogenous bgl genes (Fig. S5A). To purify such BGLs, we amplified the coding sequence (CDS) fragments of bgl from the cDNA of E. faecium CAU369 and cloned them into the pET-28a(+) vector. Subsequently, recombinant BGL proteins were expressed and characterized (Fig. S5B and C). To evaluate the activity of purified BGLs, we further measured S. Tm growth curves in cocultures with FX plus BGLs for 24 h. The growth of S. Tm was noticeably suppressed in cocultures with FX plus BGLs, compared with monocultures of FX (Fig. 3A). In addition, bgl-overexpression strains were constructed by transferring the bgl gene into the wild-type E. faecalis JH2-2 (Fig. S5D). We observed that the bgl-overexpression strains (bgl-OE#1 and bgl-OE#2) show higher expression levels of bgl and elevated hydrolytic capacity of FX (Fig. 3B and C). Taken together, these results indicate that enterococci produce BGLs to hydrolyze coumarin glycosides into antibacterial aglycones (Fig. 3D).
Bioactive aglycones inhibit S. Tm growth by chelating iron(III)
The antibacterial activity of coumarin aglycones is closely associated with their hydroxyl groups [18], prompting us to perform structure–activity relationship analysis of compounds in Table S1. Compared to compounds with no- or monohydroxyl groups (16.7%, 2 of 12), those with 2 or more hydroxyl groups (61.1%, 11 of 18) predominated among active compounds. Notably, compounds with neighboring hydroxyl groups attached to the skeleton (catechol aglycones) exhibited strong antibacterial activity, accounting for 90.9% (10 of 11) of active compounds. These catechol aglycones share structural similarity with catecholate-type siderophores, which are known to acquire iron(III) for many bacteria [29]. We hypothesized that the antibacterial mechanism of catechol aglycones depends on iron chelation.
Given that the concentration of iron required for bacterial growth is at least about 10−6 M [30], we initially measured the growth dynamics of S. Tm by adding gradient concentrations of iron(III) into iron-limited M9 minimal medium (M9) broth (Fig. 4A) to confirm the impact of iron on S. Tm growth. Iron addition significantly promoted S. Tm growth in a dose-dependent manner (Fig. 4B), while the iron chelator [2,2-dipyridyl (DIP)] limited S. Tm growth dose-dependently (Fig. 4C), highlighting the essential role of iron for S. Tm growth. In contrast, the growth of E. faecium CAU369 remained unaffected at high concentrations of DIP (Fig. 4D), consistent with a previous study demonstrating the remarkable tolerance of enterococci to iron-limited environments [6]. Similar to DIP, the catechol aglycone, FXE, inhibited various pathogenic bacteria except enterococci in a concentration-dependent manner (Fig. S6). Interestingly, excess iron(III) added into cocultures of FXE and S. Tm resulted in a remarkable color-change reaction of the broth (Fig. 4E), and the antibacterial activity of FXE was offset (Fig. 4F). These observations suggest that the antibacterial activity of catechol aglycones is dependent on iron limitation (Fig. 4G). To visualize the interaction between FXE and iron(III), we examined the effect of iron(III) on the visible absorption spectrum of FXE (Fig. 4H). Upon addition of iron(III), a peak at 741 nm of FXE appeared in a concentration-dependent manner. Likewise, esculetin and daphnetin showed similar alterations in the absorbance spectrum (Fig. S7), indicating chelation of catechol aglycones with iron(III). To assess the affinity of chelation between iron(III) and FXE, we performed an isothermal titration calorimetry (ITC) assay. The dissociation constant (KD) was 1.154 × 10−5 mol/l when the stoichiometric ratio of iron(III) to FXE was 2 (Fig. 4I), indicating that FXE has a high affinity for iron(III). Taken together, these findings denote that catechol coumarin aglycones chelate iron(III), thereby restricting the growth of S. Tm by inducing iron limitation.
Coumarin glycosides reverse the driving role of enterococci in enteric infections
Given that bioactive aglycones are frequently stored in plant cells as inactive glycosides [31], the hydrolytic capacity of enterococci to glycosides prompted us to investigate its protective role in enteric infection in vivo. Using FXE or FX to treat single S. Tm infection (Fig. 5A), FXE administration (FXET) can reduce the S. Tm burdens in the cecum and liver of mice, while the FX administration (FXT) cannot (Fig. 5B), suggesting that the hydrolysis of FX by the inherent gut microbiota in mice is too mild to restrain the S. Tm infection. In contrast, we administered FXE or FX orally to coinfected mice (Fig. 5C), and either FXE or FX treatment (FXE/FX-treated) exhibited a more than 10-fold decrease in S. Tm burdens in the cecum and liver (Fig. 5D). Similar to the FXE-treated mice, the FX treatment considerably ameliorated the symptoms of coinfected mice, indicated by less weight loss (Fig. 5E), decreased injury of the large intestine and liver (Fig. 5F and Fig. S8A), and reduced organ indices of livers (Fig. 5G). Furthermore, in the FXE- or FX-treated mice, integrity loss and inflammatory cell infiltration of the cecum, colon, and liver were reduced (Fig. 5H and Fig. S8B), and levels of inflammatory factor in the cecum and liver were significantly down-regulated (Fig. 5I and Fig. S8C), compared with those in the coinfected mice. Therefore, these results demonstrate that FX is adequately hydrolyzed into FXE by enterococci in vivo and therefore combats the infection of S. Tm in the gut.
To evaluate the effect of FXE or FX treatment on the cecal microbiota in coinfected mice, we determined the composition and diversity of the cecal microbiota by 16S ribosomal RNA (rRNA) sequencing. Notably, α-diversity varied among the differentially treated mice, and, specifically, FXE/FX-treated mice had higher Shannon and Simpson indices than those of the coinfected mice (Fig. 6A). In addition, FX-treated mice had remarkably higher abundance-based coverage estimator (ACE) and Chao1 indices compared with those of the coinfected mice (Fig. 6B). These observations suggest that both FXE and FX treatments enhance the richness and diversity of the gut microbiota in coinfected mice. To measure the degree of similarity between microbial communities, we further evaluated the β-diversity. The distinct clustering of samples was displayed according to the cecal microbiota composition in the differentially treated groups (Fig. 6C). The phylum-level analysis revealed that coinfected mice dramatically increased the relative abundance of Proteobacteria in the cecal microbiota by 539.08% (P < 0.001) compared with the uninfected mice (Fig. 6D and E), suggesting that the coinfection of E. faecium and S. Tm induced dysbiosis of the cecal microbiota in mice [32,33]. In contrast, both FXE and FX treatments reduced the relative abundance of the phylum Proteobacteria by 179.45% (P < 0.001) and 142.91% (P = 0.001), respectively, compared with the coinfected mice. Furthermore, Bugbase analysis demonstrated that both FXE and FX treatments decreased the relative abundance of biofilm formation in the cecal microbiota of coinfected mice (Fig. 6F). To identify the specific bacterial taxa associated with coinfection and coumarin administration, we used the linear discriminant analysis effect size (LEfSe) analysis to reveal the enrichment of microbiota in differentially treated mice. A cladogram representative of the cecal microbiota structure displayed the predominant bacteria and the greatest differences in taxa among these groups (Fig. 6G). The results showed that the predominant bacteria of cecal microbiota in coinfected mice were Escherichia_Shigella, Rodentibacter, Salmonella, and Clostridium_sensu_stricto_1 spp. Conversely, either FXE or FX treatment suppressed these pathogenic bacteria and induced a large increase in the abundance of beneficial bacteria, including Ligilactobacillus, Romboutsia, and Turicibacter spp. (Fig. 6H). Hence, the oral administration of coumarin glycosides ameliorates microbiota dysbiosis induced by the coinfection of enterococci and S. Tm. Altogether, these findings suggest that coumarin glucosides reverse the promotion of enterococci to S. Tm infection in vivo.
Discussion
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Enterococci often play a driving role in a variety of polymicrobial infections with poor prognoses and high mortality rates. There are few effective approaches to treating such recalcitrant and antibiotic-tolerant enterococci-mediated polymicrobial infections. Here, we show that enterococci-derived BGLs hydrolyze coumarin glucosides into antibacterial aglycones, thus reversing the promotion of enterococci to pathogenic bacteria and attenuating the severity of infection (Fig. 7). These findings suggest that dietary glycosides are a promising therapeutic intervention for enterococci-associated polymicrobial infections in the gut.
Various factors such as gastric acid suppressive medication and antibiotic treatments contribute to the intestinal expansion of enterococci [13,34]. Pioneering studies have manifested that enterococcal domination promotes the colonization of other enteric pathogens and aggravates associated infections [35–38]. It is consistent with our observations that enterococci exacerbate the growth and pathogenicity of S. Tm. Our work emphasizes the influence of bacterial interactions on the pathogen with specific commensals or coinfected pathogens. The effect of polymicrobial communities can result in enhanced disease severity due to their cooperative interactions, as one of the pathogens affects the fitness of the others [10,39–41], and, therefore, targeting such cooperations may inform novel preventative and therapeutic strategies. Correspondingly, the driving role of enterococci in polymicrobial infections provides us with an emerging perspective that recognizes enterococci as potential therapeutic targets. Given the compromise of antibiotic treatments [42], common antibiotic regimens are not suitable for polymicrobial infections mediated by multidrug-resistant enterococci. Hence, we explored a diet-based strategy to treat enterococci-associated polymicrobial infections, finding that coumarin glycosides, ubiquitously distributed in plant-based foods and medicinal herbs, can significantly suppress the growth of S. Tm in the presence of enterococci. Specifically, we found that enterococci hydrolyze the glycosidic bonds of coumarin glycosides by producing BGLs, thus transforming them into bioactive aglycones. In addition, polyphenol glycosides constitute about 80% of all polyphenolic compounds in plant tissues, while aglycones are rather rare [43]. Consequently, the hydrolytic capacity of enterococci to glycosides is the limiting step for modulating antibacterial activities of glycosides in the host, implying that commensals or probiotics can be engineered or optimized to inhibit enteric infections through plant or diet-based therapeutics.
Iron is an indispensable element in almost all living organisms and catalyzes many enzymatic reactions [44]. Pathogens must acquire enough iron to infect host within the iron-restricted gut environment [29]. We investigated the antibacterial mechanism of coumarin aglycones, finding that it is dependent on iron limitation by chelating iron via their neighboring hydroxyl groups. Our work is accord with a previous study showing that outcompeting S. Tm for iron can limit its colonization in the gut [45]. Hence, these findings represent a significant step forward in understanding how gut commensals modulate the bioactivities and bioavailability of dietary components and offer an attractive target for therapeutics to treat infections of multidrug-resistant bacteria by denying them access to iron.
There are some limitations in our study as well. Although enterococci enhance dual-species biofilm formation with diverse pathogens [46], such augmentation is strain dependent. In this study, the E. faecium strain CAU369 increased dual-species biofilm formation with S. Tm strain 15E475 only, and a similar phenomenon was not observed in other dual-species macrocolonies. The deeper mechanism regarding the promotion of enterococci to S. Tm proliferation within biofilms needs to be extensively explored. In addition, although we were unable to generate a bgl-knockout enterococcal mutant lacking the ability to produce BGLs, we characterized the impact of BGLs on glycoside hydrolysis using bgl-overexpression enterococcal strains with elevated hydrolytic capacity, as indicated by the dramatically reduced population of S. Tm in cocultures with bgl-overexpression enterococcal strains plus coumarin glycoside.
In conclusion, we demonstrate that enterococci enhance the pathogenicity and expansion of S. Tm, aggravating bacterial infection in the gut. Strikingly, dietary coumarin glycosides reverse the facilitation of enterococci in S. Tm infection, inhibiting the growth of S. Tm and ameliorating the disease severity. Our findings provide novel insights for designing and advancing therapeutic approaches to combating refractory polymicrobial infections.
Materials and Methods
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Bacterial strains and reagents
All the strains and reagents used in this study are shown in Tables S2 and S3, respectively.
Biofilm assays
For crystal violet staining, either single-species or mixed-species cultures [1.5 × 106 colony-forming units (CFUs)/ml] in tryptone soy broth supplemented with 10 mM glucose (TSBG) were inoculated into 96-well flat bottom transparent microtiter plates and incubated at 37 °C under static conditions. Supernatants were discarded, and the remaining biofilm was washed twice with phosphate-buffered saline (PBS). The biofilm was then stained with crystal violet solution (0.1%, w/v) by adding 200 μl per well and incubating for 30 min. After staining, the crystal violet solution was discarded, and the microtiter plates were washed twice with PBS, followed by crystal violet solubilization with ethanol–acetone (4:1; 200 μl per well) for 30 min at room temperature. The intensity of crystal violet staining was measured by absorbance at optical density at 595 nm using an Infinite M200 Microplate reader (Tecan).
For macrocolonies, S. Tm 15E475 and E. faecium CAU369 were inoculated as single species or a mixed species in a total volume of 10 μl onto the surface of TSBG agar. The starting inoculum of S. Tm was constantly at 1.5 × 106 CFUs. Macrocolonies were excised and resuspended in PBS at 96 h after inoculation, followed by CFU enumeration by plating on selective media and RNA extraction. Single-and dual-species macrocolonies of other bacterial strains (S. aureus T144, E. coli B2, or P. aeruginosa PAO1) were also inoculated as described above.
Minimum inhibitory concentration determination
Minimum inhibitory concentrations (MICs) of different compounds were determined using the broth microdilution method according to the CLSI 2021 guidelines and a previous study [47]. Briefly, all assayed compounds were 2-fold diluted in Mueller Hinton broth and equally mixed with bacterial suspensions. The mixtures were incubated for 16 to 18 h at 37 °C. MIC values were identified as the lowest concentrations of compounds with no visible bacterial growth.
Quantitative reverse transcription polymerase chain reaction analysis
Total RNA of S. Tm 15E475 or enterococcal strains [wild-type, empty vector (EV), bgl-OE#1, and bgl-OE#2] was extracted using the SteadyPure Universal RNA Extraction Kit. Reverse transcription of 1 μg of extracted RNA was performed using the Evo M-MLV Mix Kit following the manufacturer's protocol and a previous report [48]. Relative mRNA levels of VGs related to T3SS-1 were normalized to the expression of the housekeeping gene (rpoD) in S. Tm. Relative mRNA levels of bgl in enterococcal isolates were normalized to the expression of the reference gene (16S rRNA). The primers are shown in Table S4.
Mouse experiments
Female C57BL/6 mice aged 8 to 10 weeks (18 to 20 g) were obtained from Beijing Vital River Laboratory Animal Technology Co. Ltd. Mice were maintained in strict compliance with relevant regulations and guidelines (ID: SKLAB-B-2010-003). The usage of experimental animals is authorized by the Beijing Association for Science and Technology with the license number SYXK-2016-0008.
The S. Tm-infected mouse model was established and modified according to previous studies [45,49]. Mice were colonized with E. faecium CAU369 or S. Tm 15E475 alone as the mono-infected control. Briefly, in the mono-infected group, mice received streptomycin [antibiotic-treated (Abx); 100 μl of a solution (200 mg/ml) in sterile PBS] at 24 h before being colonized with 107 CFUs S. Tm 15E475 or 109 CFUs E. faecium CAU369. In the coinfected group, Abx-pretreated mice were precolonized with 109 CFUs E. faecium CAU369 for 24 h prior to colonization with 107 CFUs S. Tm 15E475. In the FXET/FXT groups, mice were administered FXE (100 mg/kg) or FX (100 mg/kg) at 24 h after the infection of S. Tm 15E475. In the FXE/FX-treated groups, mice were administered FXE (100 mg/kg) or FX (100 mg/kg) at 24 h after the coinfection of E. faecium CAU369 and S. Tm 15E475. Mice were euthanized by cervical dislocation at 96 h after S. Tm infection, and tissue samples including intestinal segments and livers were collected. The intestinal contents and liver tissues were homogenized and weighed, and dilutions were plated on Salmonella chromogenic agar containing selective antibiotics plates (ampicillin, 50 μg/ml) to obtain CFU per gram of tissues. The feces of single E. faecium-infected mice were collected at 12, 24, 48, and 96 h after infection, serially diluted, and plated onto Enterococcus agar containing selective antibiotics plates (ampicillin, 50 μg/ml) to determine the bacterial load. Meanwhile, cecal contents were collected for the evaluation of the abundance and diversity of intestinal microbiota.
Microbial sequencing
Cecal contents were collected into sterile tubes and stored at −80 °C. The gut microbial analysis was determined according to previous work [50]. Briefly, bacterial genomic DNA was extracted from cecal contents and the amplicons of the V3–V4 region within the 16S rRNA gene were sequenced using optimized primers (338F, 5′-ACTCCTACGGGAGGCAGCAG-3′; 806R, 5′-GGACTACHVGGGTWTCTAAT-3′) by the Illumina MiSeq platform. Sequences with ≥97% similarity were clustered, and operational taxonomic units (OTUs) were assigned for each representative sequence in the cluster. LEfSe was performed from the phylum to genus level to identify representative species of the cecal microbiota. The LEfSe analysis uses the nonparametric factor Kruskal–Wallis and rank test and then uses the (unpaired) Wilcoxon rank sum test to identify the most diverse abundance taxa.
Hematoxylin and eosin staining
Mouse tissues were fixed in a 4% paraformaldehyde solution for at least 1 week, subsequently processed for paraffin embedding, sectioned at 5 μm, and stained with hematoxylin and eosin.
Growth curves of bacteria
To test the ability of S. Tm and E. faecium to grow under iron-limited conditions, different concentrations of DIP were added to the broth of S. Tm 15E475 and E. faecium CAU 369, respectively, for 24 h. To evaluate the antibacterial activity of FXE, gradient concentrations of FXE were added to the broth of diverse Gram-negative/positive bacterial strains used in this study, respectively, for 24 h. To measure the effect of iron on S. Tm growth, iron(III) chloride hexahydrate (Sigma-Aldrich) was added into M9 broth containing S. Tm 15E475 at final concentrations of 0, 10−6, 10−5, and 10−4 M, respectively, for 24 h. To evaluate the hydrolytic ability of BGLs on FX, S. Tm 15E475 was inoculated in brain heart infusion (BHI) broth containing BGLs (2 mg/ml) and FX (6 mM). All growth curves were measured at a wavelength of optical density at 600 nm with an interval of 1 h using the Infinite M200 Microplate reader (Tecan).
Bacterial viability assay
To explore the role of iron in the antibacterial activity of FXE, S. Tm 15E475 was inoculated with excess iron(III) chloride hexahydrate at a final concentration of 10−2 M. To explore the hydrolytic capacity of enterococcal isolates, a mixture of the tested enterococcal isolate (1.5 × 109 CFUs/ml), FX (6 mM), and S. Tm 15E475 (1.5 × 106 CFUs/ml) was inoculated in BHI broth at 37°C with a shaking speed of 200 rpm for 12 h. Subsequently, 10-fold serially diluted suspensions were plated on Salmonella chromogenic agar plates at 0, 4, 8, and 12 h for overnight incubation at 37 °C.
Whole-genome sequencing
Genomic DNA was extracted from the E. faecium isolates according to the manufacturer's instructions and a previous study [51]. The obtained DNA was sequenced by Illumina Seq, and the whole genomes were aligned with the National Center for Biotechnology Information databases to search for the bgl gene with similarities ranging from 70% to 100% identity. The phylogenetic tree of E. faecium isolates based on the core genome sequences was constructed using Harvest version 1.1.2.
Ultraviolet-visible spectroscopy
The assay was performed in a transparent 96-well plate with a final volume of 200 μl. Tested compounds (FXE, esculetin, or daphnetin) were incubated with gradient concentrations of iron(III) in buffer [5% dimethyl sulfoxide (DMSO); pH 7.0] for 1 h at room temperature in the dark without shaking. Afterward, ultraviolet-visible (UV-Vis) spectrum was recorded using an Infinite M200 microplate reader (Tecan). UV-Vis spectra of the sole iron(III) served as the negative control. UV-Vis spectra of salicylic acid (1 mM) incubated with gradient concentrations of iron(III) served as the positive control.
ITC analysis
Calorimetric experiments were conducted to evaluate the interaction between FXE and iron(III) using the affinity ITC (TA Instruments) at 25 °C. Both iron(III) chloride hexahydrate (2 mM) and FXE (1 mM) were dissolved in 5% DMSO buffer. Sequential injections of iron(III) into the calorimetric cell filled with FXE were repeated 20 times with equilibration intervals of 200 s. The obtained data were processed to calculate the equilibrium dissociation constant (KD), stoichiometry (n), and changes in enthalpy (ΔH) and entropy (ΔS).
Purification of recombinant proteins
The CDS fragments of the bgl gene were amplified from the cDNA of E. faecium CAU369 and subsequently cloned into the pET-28a(+) vector. The recombinant proteins were expressed in E. coli BL21(DE3) and protein expression was induced by the addition of 0.1 mM isopropyl-β-d-thiogalactopyranoside at 28 °C for 4 h. Cells were harvested and resuspended in buffer A [20 mM tris-HCl (pH 8.0), 150 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, and 1 mM dithiothreitol] and lysed by sonication. Cell lysates were centrifuged at 12,000 rpm for 10 min, and the soluble supernatant was applied to HisTrap HP. His-BGL fusion protein was eluted with 100 and 500 mM imidazole in buffer A. Proteins were characterized by SDS-polyacrylamide gel electrophoresis gel with Coomassie blue staining and Western blot analysis with anti-His antibody. The target His-BGL fusion protein was separated from the gel and dissolved in buffer.
Matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight MS sample preparation and data acquisition
Under the premise of protein purification, the target band obtained by SDS-polyacrylamide gel electrophoresis was digested in-gel for preparing the mass spectrometer sample. Subsequently, the purified chitosanase was analyzed with a matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight MS (ultrafleXtreme, Bruker Daltonic Inc.). The raw data were converted to MASCOT generic files using protein identification software. The MS/MS fragmentation of a unique peptide sequence of the purified BGL protein was determined as NDFLWGGAVAAHQLEGGWDQGGK via MASCOT search engine (http://www.matrixscience.com/). The peptide sequencing was identified by the National Center for Biotechnology Information database (http://blast.ncbi.nlm.nih.gov/Blast.cgi) as the 6-phospho-β-glucosidase (WP_002355206.1).
Construction of the bgl-overexpression enterococcal strain
The bgl-overexpression enterococcal strain was constructed according to a previous study [47]. Briefly, the CDS of bgl was cloned into the pAM401 plasmid from the cDNA of E. faecium CAU369. The overexpression plasmid pAM401 + bgl and the empty plasmid pAM401 were separately transferred into E. faecalis JH2-2 by electroporation. Subsequently, the transformants were selected on BHI agar containing chloramphenicol (50 μg/ml) at 37 °C for 24 to 48 h and further validated by polymerase chain reaction to confirm the presence of bgl and cat genes. Finally, the expression levels of the bgl in the overexpression strain relative to internal the 16S rRNA gene were confirmed by reverse transcription polymerase chain reaction. All primers used are listed in Table S4.
Measurement of iron in diverse media by inductively coupled plasma MS
Distilled water, M9, Lilly–Barnett, and BHI media were autoclaved to eliminate all bacteria. Samples were diluted in a proportion of 1:50 in a solution containing 0.5% (v/v) of nitric acid and 0.01% (v/v) of Triton X-100. The amount of iron in these media was measured by inductively coupled plasma MS as previously described [52].
LC-MS/MS analysis
To assess the hydrolytic capacity of different enterococcal isolates, mixtures comprising enterococcal isolates with FX, esculin, or daphnin were cocultured in BHI broth at 37 °C for 12 h. Following incubation, all mixtures were voluted and then centrifugated at 14,000 rpm at 4 °C for 15 min. The supernatants were collected and diluted in methanol for LC-MS/MS analysis. The LC-MS apparatus (LCMS-8045, Kyoto, Japan) was equipped with a Waters XBridge BEH Amide column (particle size, 2.5 μm; dimensions, 2.1 mm × 100 mm; product number 186006091) maintained at an oven temperature of 35 °C and a flow rate of 0.3 ml/min. Acetonitrile (A) and 0.1% formic acid water (B) were used as mobile phases. The gradient elution conditions were as follows: 0.01 min, 95% B; 1.0 to 5.0 min, 95% to 0% B; 5.0 to 6.0 min, 0% B; 6.0 to 6.10 min, 0% to 95% B; and 6.10 to 8.0 min, 95% B. Finally, an aliquot of the supernatant (10 μl) was injected for LC-MS/MS analysis. Mass conditions of FXE and FX are provided in Table S5.
Enzyme-linked immunosorbent assay
Cecal tissues were collected for the detection of inflammatory factors [tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10)] levels using a competitive enzyme-linked immunosorbent assay. All tests were performed according to the manufacturer's instructions. The data are expressed as protein at picograms per milligram for TNF-α and IL-10 levels.
Statistical analysis
Statistical details of each experiment are provided in figure legends. The differences were analyzed by one-way analysis of variance (ANOVA) with the least significant difference (LSD) post hoc test for multiple-groups or independent-samples t test for 2 groups. A P value equal to or below 0.05 was considered statistically significant. The sample size (n) was indicated in the figure legends.
Funding
Less
  • National Key Research and Development Program of China(2022YFD1801600)
  • National Natural Science Foundation of China (32230106)
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doi: 10.34133/research.0374
  • Receive Date：2023-12-27
  • Online Date：2025-07-24
  • Published：2024-05-16
Article Data
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  • Received：2023-12-27
  • Accepted：2024-04-10
Funding
National Key Research and Development Program of China(2022YFD1801600)
National Natural Science Foundation of China (32230106)
Affiliations
    1National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
    2Engineering Research Center of Animal Innovative Drugs and Safety Evaluation, Ministry of Education, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
    3Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing 100193, China.

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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