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In this study, 75% ethanol solution was used to prepare the total extracts of arecanut nut (ANE). Then different fractions of the extracts were prepared by fractionating with petroleum ether (PE-ANE), ethyl acetate (EAC-ANE), n-butanol (BU-ANE) and water (W-ANE) in turn. The inhibition of hypertension from ANE was determined by angiotensin converting enzyme (ACE) activity inhibition assay in vitro. The inhibitory was evaluated with the fraction displayingthe best ACE inhibitory activity by the enzyme kinetic method. In addition, the contents of total polyphenols, total sugar and total protein in each fraction of ANE were determined. The correlation between the contents of the compositions and ACE inhibitory activities was analyzed. Finally, phenoliccomponents in the four fractions were analyzed. The results showed that all the fractions of ANE at different polar parts exhibited ACE inhibitory activity, and EAC-ANE displayed the best. Enzyme kinetics tests showed that the inhibitory effect of EAC-ANE from tender areca on ACE was a mixed type of competitive inhibition and non-competitive inhibition. The correlation analysis showed that the IC50 value of the ACE inhibitory activity of each polar part of ANE was significantly negatively correlated with the content of polyphenols and total sugar. And the absolute value of the correlation coefficient between the IC50 value of ACE inhibitory activity and the polyphenol content reached a very strong correlation (r=-0.912). And a variety of polyphenol components such as catechin, L-epicatechin, procyanidin B2, protocatechuic acid, and quercetin were identified in the four different polar parts, and the content of polyphenol components in EAC-ANE was the highest. In conclusion, ANE has a certain blood pressure lowering potential, and the ethyl acetate extraction site is the best, and the polyphenol content has a strong correlation with the ACE inhibitory activity of each extract. It can be preliminarily inferred that polyphenols may be the main substances that inhibit the activity of ACE in ANE.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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