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Verification of the Interaction Between Cassava MeHsfB3b Transcription Factor and MeFKBP20 Protein
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Chaoqun WANG1, Linlin LI1, Yinhua CHEN1, Xiaofei ZHANG3, Yuan YAO2, *, Mengting GENG1, *
Chinese Journal of Tropical Crops | 2024, 45(3) : 450 - 458
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Chinese Journal of Tropical Crops | 2024, 45(3): 450-458
• Omics & Biotechnology •
Verification of the Interaction Between Cassava MeHsfB3b Transcription Factor and MeFKBP20 Protein
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Chaoqun WANG1, Linlin LI1, Yinhua CHEN1, Xiaofei ZHANG3, Yuan YAO2, *, Mengting GENG1, *
Affiliations
  • 1.College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Institute for Tropical Agricultural, Haikou, Hainan 571101, China
  • 3.International Center for Tropical Agriculture, Cali AA6713, Colombia
Published: 2024-03-25 doi: 10.3969/j.issn.1000-2561.2024.03.002
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The heat shock transcription factor MeHsfB3b is important in regulating the resistance to cassava bacterial blight. In the previous study, the candidate interaction protein MeFKBP20 of MeHsfB3b was obtained by the yeast two-hybrid technology, which belongs to the FKBP-type peptide prolyl cis-trans isomerase gene family. In this study, the full-length MeFKBP20 gene was cloned to be 561 bp, encoding 186 amino acids, with a molecular weight of 19.99 kDa and a FKPB_C domain. The expression pattern analysis showed that MeFKBP20 was highly expressed in mature leaves and roots of cassava, and the expression level of young leaves and petioles was low. It could quickly respond to the in-duction of pathogen XpmCHN11 and maintain a high expression abundance, suggesting that it is involved in response to pathogen infection. The interaction between MeHsfB3b protein and MeFKBP20 protein through 201-241 aa region was proved by the yeast two-hybrid point-to-point and bimolecular fluorescence complementation experiments. The results of this study are helpful to further analyze the mechanism of MeHsfB3b gene regulating cassava resistance to cassava bacterial blight.

heat shock transcription factor  /  FKBP-type peptide prolyl cis-trans isomerase  /  yeast two-hybrid  /  bimolecular fluorescence complementation
Chaoqun WANG, Linlin LI, Yinhua CHEN, Xiaofei ZHANG, Yuan YAO, Mengting GENG. Verification of the Interaction Between Cassava MeHsfB3b Transcription Factor and MeFKBP20 Protein[J]. Chinese Journal of Tropical Crops, 2024 , 45 (3) : 450 -458 . DOI: 10.3969/j.issn.1000-2561.2024.03.002
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.03.002
  • Receive Date：2023-02-09
  • Online Date：2026-06-26
  • Published：2024-03-25
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History
  • Received：2023-02-09
  • Revised：2023-03-14
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Affiliations
    1.College of Tropical Crops, Hainan University, Haikou, Hainan 570228, China
    2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Institute for Tropical Agricultural, Haikou, Hainan 571101, China
    3.International Center for Tropical Agriculture, Cali AA6713, Colombia
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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