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Screening of MeCML42 Interacting Proteins in Cassava
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Huimin ZHANG1, 2, 3, Yujian GE1, 2, 3, Jingyi HOU1, 2, Baojuan LIANG1, 2, 3, Zhixin REN1, 2, 3, Jianchun GUO2, 3, Yuan YAO2, 3, Yajie WANG2, 3, *, Mengting GENG1, *
Chinese Journal of Tropical Crops | 2024, 45(11) : 2251 - 2257
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Chinese Journal of Tropical Crops | 2024, 45(11): 2251-2257
• Omics & Biotechnology •
Screening of MeCML42 Interacting Proteins in Cassava
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Huimin ZHANG1, 2, 3, Yujian GE1, 2, 3, Jingyi HOU1, 2, Baojuan LIANG1, 2, 3, Zhixin REN1, 2, 3, Jianchun GUO2, 3, Yuan YAO2, 3, Yajie WANG2, 3, *, Mengting GENG1, *
Affiliations
  • 1.College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
  • 3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572000, China
Published: 2024-11-25 doi: 10.3969/j.issn.1000-2561.2024.11.002
Outline
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Cassava (Manihot esclenta Crantz) has excellent planting characteristics such as drought resistance, barren tolerance and strong adaptability, and is the main food crop for more than 1 billion people in the world. CMLs (calmodulin-like proteins) are plant calmodulin-like receptors in cells. They interact with other calmodulin-binding proteins to regulate cellular physiological processes and participate in plant stress response. MeCML42 gene in cassava is induced by drought stress. In order to study the drought stress response pathway involved in this gene, yeast two-hybridization was used to screen the interacting proteins of MeCML42. Firstly, the decoy vector pGBKT7-MeCML42 was constructed by homologous recombination technique, and the self-activation experiment showed that MeCML42 protein had no self-activation activity. The toxicity test showed that MeCML42 protein was not toxic to yeast and did not affect the normal growth of yeast. The cDNA library was screened by yeast two-hybridization and 7 candidate interacting proteins were obtained. The interaction between MeCML42 and thioredoxin MeCDSP32 was verified by yeast double heterogyclic experiment. Mannitol simulation of drought stress showed that the expressions of MeCML42 and MeCDSP32 were both induced by drought, and the expression levels were the highest at 48 h under stress. These results indicated that MeCML42 and MeCDSP32 were synergistically involved in cassava's response to drought stress. The results of this study provide a new basis for MeCML42 to participate in cassava response to drought stress.

cassava  /  MeCML42  /  yeast two-hybrid  /  MeCDSP32
Huimin ZHANG, Yujian GE, Jingyi HOU, Baojuan LIANG, Zhixin REN, Jianchun GUO, Yuan YAO, Yajie WANG, Mengting GENG. Screening of MeCML42 Interacting Proteins in Cassava[J]. Chinese Journal of Tropical Crops, 2024 , 45 (11) : 2251 -2257 . DOI: 10.3969/j.issn.1000-2561.2024.11.002
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.11.002
  • Receive Date：2024-03-07
  • Online Date：2026-06-26
  • Published：2024-11-25
Article Data
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History
  • Received：2024-03-07
  • Revised：2024-03-29
Funding
Affiliations
    1.College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
    3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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