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Cloning, Expression Analysis, and Functional Verification of the MePCS1 Gene in Cassava
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Baojuan LIANG1, 2, 3, Yujian GE1, 2, 3, Jingyi HOU1, 2, Huimin ZHANG1, 2, 3, Zhixin REN1, 2, 3, Mengting GENG1, Yajie WANG2, 3, Jianchun GUO2, 3, *, Yuan YAO2, 3, *
Chinese Journal of Tropical Crops | 2024, 45(11) : 2243 - 2250
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Chinese Journal of Tropical Crops | 2024, 45(11): 2243-2250
• Omics & Biotechnology •
Cloning, Expression Analysis, and Functional Verification of the MePCS1 Gene in Cassava
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Baojuan LIANG1, 2, 3, Yujian GE1, 2, 3, Jingyi HOU1, 2, Huimin ZHANG1, 2, 3, Zhixin REN1, 2, 3, Mengting GENG1, Yajie WANG2, 3, Jianchun GUO2, 3, *, Yuan YAO2, 3, *
Affiliations
  • 1.College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
  • 3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences / National key Laboratory for Tropical Crop Breeding, Sanya, Hainan 572000, China
Published: 2024-11-25 doi: 10.3969/j.issn.1000-2561.2024.11.001
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Phytochelatin synthase (PCS) is a key enzyme that catalyzes the synthesis of phytochelatin (PC). PC can reduce the toxicity of heavy metals to plants through complexation. Heavy metal pollution in soil increases the risk of cassava food safety. It is of great significance to study the reduction and control of heavy metals in cassava by analyzing the function of MePCS1 gene. In this study, the coding region of MePCS1 gene in cassava variety SC8 was cloned by PCR. The full length was 1512 bp, encoding 503 amino acids. Protein sequence analysis showed that MePCS1 protein was a hydrophilic protein, containing 41 phosphorylation sites and 2 glycosylation sites, without signal peptide. The phylogenetic tree showed that the cassava MePCS1 protein had the closest relationship with the PCS protein of the rubber tree. The qPCR analysis showed that the MePCS1 gene had the highest expression level in the stem. During the development of cassava roots, the expression level of MePCS1 gene was the highest in the root enlargement period. Lead (Pb) stress induced MePCS1 gene expression. MePCS1 gene can improve the tolerance of BY4741 yeast to Pb. The results of this study provide new information for further analysis of the functional characteristics of the MePCS1 gene and its mechanism for reducing Pb in cassava tuber roots.

phytochelatin synthases  /  heavy metal Pb stress  /  yeast expression
Baojuan LIANG, Yujian GE, Jingyi HOU, Huimin ZHANG, Zhixin REN, Mengting GENG, Yajie WANG, Jianchun GUO, Yuan YAO. Cloning, Expression Analysis, and Functional Verification of the MePCS1 Gene in Cassava[J]. Chinese Journal of Tropical Crops, 2024 , 45 (11) : 2243 -2250 . DOI: 10.3969/j.issn.1000-2561.2024.11.001
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Year 2024 volume 45 Issue 11
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.11.001
  • Receive Date：2024-04-24
  • Online Date：2026-06-26
  • Published：2024-11-25
Article Data
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History
  • Received：2024-04-24
  • Revised：2024-05-09
Funding
Affiliations
    1.College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
    3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences / National key Laboratory for Tropical Crop Breeding, Sanya, Hainan 572000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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