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Camellia nitidissima belongs to the genus Camellia of theaceae and belongs to the first-level national protection plant, with high ornamental value and medicinal value. However, at present, there are few wild resources of C. nitidissima. It is necessary to find efficient artificial cultivation methods, rapidly propagate excellent C. nitidissima seedlings, and then greatly improve the yield of large-scale cultivation of C. nitidissima. Arbuscular mycorrhizal (AM) fungi can significantly promote plant growth and development, increase crop yield, and enhance plant stress and disease resistance. Inoculation of AM fungi into C. nitidissima has a positive effect on the cultivation of C. nitidissima. Since almost all AM fungal inoculation test involves the growth of mycorrhizal observation and infection rate determination, and AM infection situation observation methods available are not efficient. An efficient dyeing of AM fungi C. nitidissima was reported. In this study, the effects of five dyes (Sudan red Ⅳ, acid fuchsin, trypan blue, aniline blue and acetic acid ink) on AM fungi of healthy plant roots of C. nitidissima were investigated. The results showed that 5% acetic acid ink staining solution had the best staining effect. Mycelia, vesicles, spores and other structures of AM fungi in root cortex cells were clearly visible, and AM fungi could be clearly distinguished from other unknown fungi, and the root staining effect could be preserved for a long time. The mycelia and vesicles of AM fungi stained with Sudan red Ⅳ and acid fuchsin were not in obvious contrast with the background, and the preservation time of acid fuchsin staining was short, which was not conducive to observation. The dyeing effect of aniline blue and trypan blue was slightly inferior to acetic acid ink, and the price was expensive and the experimental cost was high. The last four dyes are suspected of causing cancer, and the long-term use has great potential safety risks to the health of operators. Acetic acid ink dyeing is simple, low toxicity, low cost and excellent dyeing effect. It is suitable for the staining and section observation of AM fungi in the roots of C. nitidissima. The results would provide new ideas for the future application of C. nitidissima mycorrhizal seedlings.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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