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To further explore the molecular characteristics and pathogenicity of Sri Lankan cassava mosaic virus (SLCMV) isolates in China. Mosaic cassava leaves were detected, and the domestic SLCMV isolates full-length genomes of DNA-A and DNA-B were obtained by PCR amplification. It`s nucleic acid and amino acid sequence characteristics were analyzed by bioinformatics software. The infectious clones of strong and weak pathogenic isolates (SLCMV-Colombo and SLCMV-DG1922) were constructed. The DNA-A and DNA-B components of the two virulence isolates were recombined, and the pathogenicity differences of the two isolates were compared by tobacco (Nicotiana tabacum) inoculation. The results showed that SLCMV isolates belong to “Old World begomoviruses” encoding 8 open reading frames (ORFs) including the AV2 gene. It has a typical common region (CR), repeat sequence, TATA Box and TAATATT↓AC stem-loop structure of Geminiviruses, and 7-amino-acid are missing at the carboxyl terminal of Rep protein. Domestic SLCMV genome, coding and non-coding region sequences were 97.0%-100.0% similar to the isolates from Cambodia, Thailand and Vietnam, and 86.5%-98.6% similar to the early isolates from India and Sri Lanka. Coding region of DNA-B were 95.0%-97.6% similar to Indian cassava mosaic virus strain (ICMV), were less than 80.0% to the other 9 African cassava mosaic virus strains. Infection clone inoculation showed that the DNA-A (ColA) component of the strong virulent isolate SLCMV-Col played an important role in the typical mosaic symptoms of N. tabacum leaves, while the DNA-B (DG1922-B) of the domestic isolate could cooperate with Col-A to produce strong pathogenicity to tobacco plants. This study analyzed the whole genome structure of SLCMV in China, the infectious clone and its technology provide an important research basis for further pathogenesis research of SLCMV.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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