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Differential Expression of TCS1 Allele Involved in the Formation of Low Caffeine in Nongdao Yecha
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Yufei LIU, Dandan PANG, Chunlin CHEN, Yiping TIAN, Yunnan SUN, Youyong LI, Linbo CHEN**
Chinese Journal of Tropical Crops | 2024, 45(1) : 23 - 29
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Chinese Journal of Tropical Crops | 2024, 45(1): 23-29
• Omics & Biotechnology •
Differential Expression of TCS1 Allele Involved in the Formation of Low Caffeine in Nongdao Yecha
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Yufei LIU, Dandan PANG, Chunlin CHEN, Yiping TIAN, Yunnan SUN, Youyong LI, Linbo CHEN**
Affiliations
  • Tea Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation / Yunnan Provincial Key Laboratory of Tea Science, Menghai, Yunnan 666201, China
Published: 2024-01-25 doi: 10.3969/j.issn.1000-2561.2024.01.003
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The alkaloid content of different samples in different seasons and different developmental stages of MHLC of two tea varieties, Nongdao Yecha (MHLC) and Yunkang 10 (YK10) was determined by HPLC. Caffeine synthase 1 gene (TCS1) was cloned from the cDNA of tea plant by RT-PCR in different samples, and its expression level was analyzed. The results showed that MHLC was a natural tea germplasm resource with low caffeine and high theobromine. Theobromine content of MHLC in different seasons was greater than 23.00 mg/g, while the caffeine content was less than 4.00 mg/g. In addition, the accumulation of caffeine and theobromine in MHLC shoots was the highest, and the leaves at the same stage had higher caffeine and theobromine than stems, and the caffeine and theobromine in leaves and stems gradually decreased with the increase of maturity. Through gene cloning and quantitative analysis, it was found that MHLC had two TCS1 allelic variants: TCS1b (with only theobromine synthase activity) and TCS1d (with both theobromine and caffeine synthase activities). Among them, the high expression of TCS1b and the extremely low expression of TCS1d are likely to be the key reasons for MHLC high theobromine and low caffeine. This research would provide material for low-caffeine tea breeding and molecular basis for the efficient utilization of MHLC.

Nongdao Yecha  /  specific resources  /  TCS1  /  biosynthesis
Yufei LIU, Dandan PANG, Chunlin CHEN, Yiping TIAN, Yunnan SUN, Youyong LI, Linbo CHEN. Differential Expression of TCS1 Allele Involved in the Formation of Low Caffeine in Nongdao Yecha[J]. Chinese Journal of Tropical Crops, 2024 , 45 (1) : 23 -29 . DOI: 10.3969/j.issn.1000-2561.2024.01.003
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doi: 10.3969/j.issn.1000-2561.2024.01.003
  • Receive Date：2022-10-12
  • Online Date：2026-06-26
  • Published：2024-01-25
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  • Received：2022-10-12
  • Revised：2023-01-04
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    Tea Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation / Yunnan Provincial Key Laboratory of Tea Science, Menghai, Yunnan 666201, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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