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With the development of the aerospace industry, the method of combining traditional breeding technology with aerospace mutagenesis technology to achieve breeding goals has been widely recognized by breeding experts. The problems faced by the scale of the banana industry in Fujian make the breeding of new banana varieties an important issue. In the early stage of this study, Brazil banana was used as the female parent. On the basis of space mutation, through continuous cultivation, screening and observation, two materials with obvious differences in biological and morphological characteristics were selected. In order to identify the genetic specificity of the two independently bred aerospace banana materials, the ISSR molecular marker technology was used in this study to screen out two primers with good polymorphism from 30 primers to analyze the two aerospace banana materials and 18 existing banana varieties. A total of 17 clear DNA bands were amplified by the two primers screened, of which 13 were polymorphic bands, accounting for 76.47% of the total bands, showing a high polymorphism at the DNA molecular level of banana materials. Electrophoresis showed that, combined with ISSR primers UBC881 and UBC847, the two aerospace banana materials had specific amplification bands, which could be distinguished from the other 18 banana varieties. Cluster analysis results showed that the genetic similarity coefficients between the 20 banana germplasm materials ranged from 0.545 to 1.000, with an average of 0.905; when the genetic similarity coefficient was 0.960, there were obvious genetic differences between the two bred aerospace banana materials, and the two bred aerospace banana materials with the female parent, and with other 17 banana germplasms. The results of this study would provide a strong basis for the breeding and identification of new varieties of aerospace bananas.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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