This study was conducted to explore the cumulative demand of nitrogen, phosphorus and potassium in disease-free sugarcane original seedlings, and provide reference for the nutrient management in the process of field propagation of disease-free sugarcane original seedlings. The growth of the disease-free sugarcane original seedlings of Zhongtang No. 1 in 2020 and 2021 was investigated, and the biomass and contents of nitrogen, phosphorus and potassium in roots, stems and leaves at various growth stages were determined for further analysis of the accumulation, phase absorption and daily absorption rate of nitrogen, phosphorus and potassium. It took 6 to 7 months for disease-free sugarcane original seedlings to reach 15 stem nodes from temporary planting of seedlings to the best harvest of seed canes. From July, August to late September (stem 1 to 2), the growth of sugarcane stems was the fastest. The cumulative increase of dry matter was the fastest in stem 4 (late October to late November), and this is the key stage of biomass accumulation. During the whole growth period of the disease-free sugarcane original seedlings, the content and accumulation of each element from high to low were potassium > nitrogen > phosphorus. Among the organs of roots, stems and leaves, leaves and stems always had higher nitrogen, phosphorus and potassium contents. The accumulation of nitrogen, phosphorus and potassium was mainly stored in the leaves before the stem elongation stage 1, and then mainly stored in the stems. The nutrient uptake rates of nitrogen, phosphorus and potassium at the seedling stage and tillering stage was 24.84%, 26.18% and 27.74%, respectively, and it was 55.54%, 58.61% and 52.30% at the stem 1 to 3 (late July to late October), while 19.62%, 15.20% and 19.96% during stem 4 and 5 (late October to late December). Reasonable fertilization can be carried out according to the phase absorption of each element, thereby improving the quality and economic benefits of disease-free sugarcane seedlings production. The demands of nitrogen, phosphorus and potassium in the breeding process of disease-free sugarcane original seedlings were clarified, which would provide a reference for the scientific and reasonable fertilization in large-scale field propagation of disease-free sugarcane original seedlings in the future, and improving the efficiency of the propagation and nutrient utilization of disease-free original seedlings.