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The hyperosmotic Ca2+ channel protein OSCA is crucial for controlling hyperosmotic stressors in plants. To understand the role of the OSCA gene family in tea plant response to drought stress, this study was conducted to identify the OSCA gene family based on the whole genome data of tea plant and analyze the gene and protein structures and promoter cis-acting element, and analyze the expression patterns of CsOSCAs in different tissues of tea plant, among different drought-resistant varieties and under drought stress. The results showed that the tea plants genome contained 12 members of the OSCA gene family, named CsOSCA1-CsOSCA12. The amino acid sequences encoded by the CsOSCAs were 667-831 bp in length, with protein molecular weight ranging from 76 630.55 kDa to 93 563.99 kDa and isoelectric points ranging from 6.15 to 9.33, containing 9-12 transmembrane structural domains, all containing the characteristic conserved structural domain DUF221, which could be divided into four subfamilies based on phylogenetic relationships. Ten CsOSCAs were localized to seven chromosomes of Camellia sinensis and two CsOSCAs were localized to the contig of unanchored chromosomes. The secondary structure of the CsOSCAs contained 32%-38% transmembrane structure and 60%-68% α-helix. The CsOSCAs were tissue expression specific, and CsOSCA2, CsOSCA3, CsOSCA11, and CsOSCA12 were significantly higher in the drought-sensitive variety CN98 than those in the other two drought-tolerant varieties. Nine genes were responded to PEG stress, among which CsOSCA2, CsOSCA3, CSOSCA5, CsOSCA8, CsOSCA10, and CsOSCA12 were strongly induced by drought stress. Six genes included drought-inducible response elements, and 11 genes contained abscisic acid response elements, according to further examination of the promoter cis-acting elements. It is hypothesized that the OSCA gene family of tea plants play an important role in the response to drought stress, and this study would provide a reference for the functional analysis and stress resistance of OSCA gene in tea plant.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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