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The induction of adventitious roots (AR) is a key step for successful clonal propagation in plants. Previous studies have shown that the auxin polar transport carrier MiPIN1 plays an important role in AR formation from mango cotyledon segments, yet its upstream regulatory mechanisms remain unclear. In this study, a 2 112 bp promoter sequence of MiPIN1 isolated using FPNI-PCR. Bioinformatic analysis revealed that the promoter region contained various cis-acting elements, including those involved in light response, MYB binding, abscisic acid response, and low-temperature stress. A bait vector, pAbAi-MiPIN1, was constructed, and the minimal concentration of Aureobasidin A (AbA) required to suppress autoactivation in the yeast system was determined to be 500 ng/mL. Furthermore, a yeast cDNA library was constructed from mango cotyledon segments during AR formation. Using the yeast one-hybrid (Y1H) system, 62 upstream proteins that potentially bind to the MiPIN1 promoter were identified, including auxin-binding protein ABP19a-like, zinc finger protein CCCH, MYB308, 8-hydroxygeraniol dehydrogenase, and chlorophyll a-b binding protein LHCII. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that the proteins were mainly involved in biological processes such as cellular processes, metabolism, response to stimuli, and biological regulation. They were also enriched in metabolic pathways related to secondary metabolite biosynthesis, energy metabolism, and the metabolism of amino acids, lipids, and carbohydrates. The findings would lay a foundation for unraveling the molecular regulatory network underlying adventitious root formation in mango.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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