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Screening and Prelininary Identification of a Heat-Inducible Promoter of Hevea
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Wenping WANG1, Xiaomei LUO1, Jie CAO1, 2, Shengmin ZHANG1, 2, Jiyan QI1, 2, Yi ZHANG1, 2, *
Chinese Journal of Tropical Crops | 2025, 46(5) : 1025 - 1031
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Chinese Journal of Tropical Crops | 2025, 46(5): 1025-1031
• Omics & Biotechnology •
Screening and Prelininary Identification of a Heat-Inducible Promoter of Hevea
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Wenping WANG1, Xiaomei LUO1, Jie CAO1, 2, Shengmin ZHANG1, 2, Jiyan QI1, 2, Yi ZHANG1, 2, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry (Agriculture and Rural College, Rural Revitalization College), Hainan University, Haikou, Hainan 570228, China
  • 2.Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China
Published: 2025-05-25 doi: 10.3969/j.issn.1000-2561.2025.05.001
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Genetic engineering technology serves as an effective method to shorten crop breeding cycles, specifically cultivate new crop varieties, and identify plant gene functions. Induced promoters are important tools in genetic engineering research, among which thermally inducible promoters being extensively utilized in plant molecular biology owing to the simple vector structure, convenient induction, and low cost. At present, the development of genetic engineering technology in rubber trees lags behind. Functional studies of target genes typically use constitutive promoters, which limits the progress of related work. In this study, rubber tree calli were treated by heat. Through transcriptome sequencing analysis, identified eight genes that were non-expressed or minimally expressed, but exhibited high level expression after heat induction in calli. After heat induced expression changes and specificity evaluation, two candidate genes HbHSP17.6C and HbHSP17.6B that specifically respond to heat treatment were identified. Further through nested PCR, we cloned the 1962 nt sequence upstream of the start codon of the heat shock protein gene HbHSP17.6C. Confirm that it is composed of a core transcription region and multiple cis acting elements, and is named pHbHSP17.6C. It is inferred that this promoter could be used for gene function research in rubber trees and other closely related plants, which is expected to provide new tool options for related research.

rubber tree  /  callus  /  heat-inducible promoter
Wenping WANG, Xiaomei LUO, Jie CAO, Shengmin ZHANG, Jiyan QI, Yi ZHANG. Screening and Prelininary Identification of a Heat-Inducible Promoter of Hevea[J]. Chinese Journal of Tropical Crops, 2025 , 46 (5) : 1025 -1031 . DOI: 10.3969/j.issn.1000-2561.2025.05.001
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.05.001
  • Receive Date：2024-12-03
  • Online Date：2026-06-26
  • Published：2025-05-25
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History
  • Received：2024-12-03
  • Accepted：2025-02-02
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Affiliations
    1.School of Tropical Agriculture and Forestry (Agriculture and Rural College, Rural Revitalization College), Hainan University, Haikou, Hainan 570228, China
    2.Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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