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Molecular Mechanism of Banana MaERF15 Regulating Postharvest Fruit Ripening by Directly Binding MaACO8 Promoter
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Bingyun HUANG1, 2, Yunke ZHENG2, 3, Jianbin ZHANG2, 3, Xinguo LI1, *, Juhua LIU2, 3, *
Chinese Journal of Tropical Crops | 2025, 46(5) : 1032 - 1042
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Chinese Journal of Tropical Crops | 2025, 46(5): 1032-1042
• Omics & Biotechnology •
Molecular Mechanism of Banana MaERF15 Regulating Postharvest Fruit Ripening by Directly Binding MaACO8 Promoter
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Bingyun HUANG1, 2, Yunke ZHENG2, 3, Jianbin ZHANG2, 3, Xinguo LI1, *, Juhua LIU2, 3, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • 2.National Key Laboratory for Tropical Crops Breeding / Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572024, China
  • 3.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
Published: 2025-05-25 doi: 10.3969/j.issn.1000-2561.2025.05.002
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Banana is a typical respiration fruit. MaERF15 is a transcription factor located in the nucleus and has the effect of regulating postharvest ripening of banana fruits. MaACO8 is a key ethylene biosynthesis-ACC oxidase gene expressed at high levels during postharvest ripening of banana fruits. But the molecular interaction mechanism between the two has not been clarified. To explore the transcriptional regulation mechanism of ethylene response factor MaERF15 on the key genes of downstream ethylene synthesis was to provide a theoretical basis for the innovation of postharvest preservation technology of banana fruits. In this study, the promoter sequence of MaACO8 was isolated from banana DNA, and the promoter activity of MaACO8 was studied by bioinformatics analysis and GUS staining. Yeast monohybridization, EMSA, dual fluorescein reporter system and in vivo imaging system were used to study the interaction mechanism between MaERF15 and MaACO8 promoter. The results showed that there were 11 GCC-boxes in the promoter sequence of MaACO8 that bind to AP2/ERF transcription factors, which had strong priming activity. MaERF15 could specifically bind to the GCC-box of the MaACO8 promoter, activate the expression of MaACO8, promote the biosynthesis of ethylene, and regulate fruit ripening. The results further improved the theory of postharvest ethylene biosynthesis regulating fruit ripening of banana fruits, and would provide genetic resources and theoretical basis for the research and development of new technologies for storage and transportation tolerance breeding and maturity regulation of bananas, respectively.

banana  /  MaERF15  /  MaACO8  /  transcription regulation
Bingyun HUANG, Yunke ZHENG, Jianbin ZHANG, Xinguo LI, Juhua LIU. Molecular Mechanism of Banana MaERF15 Regulating Postharvest Fruit Ripening by Directly Binding MaACO8 Promoter[J]. Chinese Journal of Tropical Crops, 2025 , 46 (5) : 1032 -1042 . DOI: 10.3969/j.issn.1000-2561.2025.05.002
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.05.002
  • Receive Date：2024-12-03
  • Online Date：2026-06-26
  • Published：2025-05-25
Article Data
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History
  • Received：2024-12-03
  • Accepted：2024-12-28
Funding
Affiliations
    1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2.National Key Laboratory for Tropical Crops Breeding / Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572024, China
    3.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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