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Mechanism of Transcription Factor APETALA3-3 Regulating Floral Morphogenesis in Dendrobium officinale Based on DAP-Seq and RNA-seq Analysis
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Yarong SONG, Hongfeng ZHU, Die AO, Anqi LIU, Deyin LU, Fengmei HE*
Chinese Journal of Tropical Crops | 2025, 46(5) : 1052 - 1063
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Chinese Journal of Tropical Crops | 2025, 46(5): 1052-1063
• Omics & Biotechnology •
Mechanism of Transcription Factor APETALA3-3 Regulating Floral Morphogenesis in Dendrobium officinale Based on DAP-Seq and RNA-seq Analysis
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Yarong SONG, Hongfeng ZHU, Die AO, Anqi LIU, Deyin LU, Fengmei HE*
Affiliations
  • College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
Published: 2025-05-25 doi: 10.3969/j.issn.1000-2561.2025.05.004
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The diversity of orchid labellum, a specialized petal, has great ornamental and biological value, but the formation mechanism has not been solved so far. APETALA3-3 (AP3-3) is a floral organ identity gene, which is related to the formation of orchid labellum, but there is no report on how it regulating the formation mechanism of orchid labellum diversity. In this project, wild type and DoAP3-3 overexpressing floral organs of Dendrobium officinale were used as the research objects to explore the formation mechanism from the perspective of transcription factor DoAP3-3 regulating downstream target genes through DAP-seq and RNA-seq joint analysis. The results showed that a total of 7 key candidate target genes, MADS6, NAC029, NAC054, WOX3, AS2, ERECTA, AG were identified, which may regulate floral organ development and floral morphogenesis development under the action of DoAP3-3 by specific regulation or interaction with other genes. The interaction between transcription factor DoAP3-3 and MADS6 was verified by yeast one-hybrid experiment, which further affecting floral morphogenesis development. This study would lay a foundation for preliminary analysis of the regulatory mechanism between DoAP3-3 and its target genes, and exploring the formation mechanism of highly specialized and diversified flower morphology in Orchidaceae.

APETALA3-3  /  Dendrobium officinale  /  DNA affinity purification sequencing  /  DAP-seq  /  RNA-seq  /  flower morphologies development
Yarong SONG, Hongfeng ZHU, Die AO, Anqi LIU, Deyin LU, Fengmei HE. Mechanism of Transcription Factor APETALA3-3 Regulating Floral Morphogenesis in Dendrobium officinale Based on DAP-Seq and RNA-seq Analysis[J]. Chinese Journal of Tropical Crops, 2025 , 46 (5) : 1052 -1063 . DOI: 10.3969/j.issn.1000-2561.2025.05.004
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Year 2025 volume 46 Issue 5
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doi: 10.3969/j.issn.1000-2561.2025.05.004
  • Receive Date：2024-12-26
  • Online Date：2026-06-26
  • Published：2025-05-25
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  • Received：2024-12-26
  • Accepted：2025-02-06
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    College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan 650201, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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