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Drought resistant microbial preparations are beneficial soil microorganisms that produce plant growth hormone to enhance the drought tolerance of plants and promote plant growth, which is an ideal substitute for traditional chemical drought resistant preparations. In this study, the bacteria obtained in the soils of Wuyishan tea plantation were used as the research object to screen strains with the high secretion indole-3-acetic acid (IAA) ability and strong drought resistance, and the culture conditions optimization and whole genome sequencing analysis were also conducted. A strain SZF7 with high secreting IAA was obtained, which belonged to Bacillus cereus, which had stronger drought resistance than other strains. The optimal culture conditions for IAA secretion of SZF7 were pH 5.0 and 37 ℃ with 24 h incubation, and pH and temperature significantly affected on the IAA secretion. In the COG and KEGG metabolic pathways of SZF7 genome, the genes related to biological metabolism accounted for 47.96% and 68.52% of the total functional genes, respectively. The genes related to plant growth promotion included IAA, siderophore, non-ribosomal peptide synthase (NRPS), lipopeptide compounds and bacillibactin. There were two IAA synthesis pathways in SZF7, one of which was indole-3-pyruvate pathway mediated by indole-3-pyruvate decarboxylase, and the other was tryptamine pathway mediated by indole-3-glycerol phosphate synthase. This study would provide a scientific basis for the application of drought-resistant and growth-promoting bacteria in the production of tea and other cash crops.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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