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This study aims to screen the microbial strains capable of producing β-D-glucosidase from fermented vanilla pods and apply the screened strains to the fermentation process of vanilla with the goal of enhancing vanillin production and improving the quality of vanilla bean. Septenary selective medium was utilized to isolate β-D-glucosidase producing bacteria, identify the homology of each bacterial strain and measure the production of β-D-glucosidase activity of each strain on two strains with higher enzyme production. The phylogenetic tree was constructed. The two strains of bacteria with the highest enzyme production in different combinations were sprayed onto the fermented vanilla pods. The content of vanillin, vanillin glucoside, and volatile components in different treatments of β-D-glucosidase producing bacteria were determined. Nine strains were successfully screened from the vanilla pods originating in Madagascar (M), and twelve strains were isolated from the vanilla pods produced by Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences (S). The fifteen strains were detected as Bacillus. M3 and S7 had higher enzyme activities than other isolates. Vanilla pods treated with M3 obtained the highest vanillin content, which reached to 2.25% and possed the highest aroma components. The maximum decrease in vanillin glucoside content was 80.7% when sprayed with M3 and S7 in a volume ratio of 1:1. There were 34 volatile substances in the fermented pods sprayed with M3, making it the most volatile combination of spraying bacteria. In the present study, we successfully isolated β-D-glucosidase producing bacteria for improving the quality of vanilla pods fermentation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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