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Screening of Biocontrol Bacteria Against Second-stage Juveniles of Meloidogyne enterolobii and Evaluation of the Growth-promoting Characteristics
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Meng CHEN1, 2, Jiawei SHANG2, Haibo LONG2, Jun WANG2, Lijia GUO2, You ZHOU2, Changcong LIANG2, Yang YANG2, Junsheng HUANG2, Laying YANG2, *
Chinese Journal of Tropical Crops | 2024, 45(10) : 2183 - 2191
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Chinese Journal of Tropical Crops | 2024, 45(10): 2183-2191
• Plant Protection & Bio-safety •
Screening of Biocontrol Bacteria Against Second-stage Juveniles of Meloidogyne enterolobii and Evaluation of the Growth-promoting Characteristics
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Meng CHEN1, 2, Jiawei SHANG2, Haibo LONG2, Jun WANG2, Lijia GUO2, You ZHOU2, Changcong LIANG2, Yang YANG2, Junsheng HUANG2, Laying YANG2, *
Affiliations
  • 1.College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430007, China
  • 2.Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / National Collection of Microbial Resource for Fertilizer (Hainan) / Collection of Tropical Agricultural Microbial Resource in Hainan Province, Haikou, Hainan 571101, China
Published: 2024-10-25 doi: 10.3969/j.issn.1000-2561.2024.10.019
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Meloidogyne enterolobii is a soil-borne disease that seriously harms crop production in tropical areas, and the agricultural losses caused by it are incalculable every year. For the control of root-knot nematodes, the traditional physical and chemical control methods have some disadvantages, while biological control is environmentally friendly and safe for people and livestock, so it has gradually become a research hotspot. This study used the impregnation method to screen strains with killing activity against the second instar larvae of M. enterolobii. The fermentation conditions and treatment time of the screened highly active strains were optimized. The strains were identified using 16S rRNA sequencing as well as physiological and biochemical methods, and growth promoting characteristics were analyzed. The research results indicated that the nematicidal activity of the fermentation supernatant of BWLY1PSB-1, BWLY2X-4 and BWLY3X-11 were more than 65%. Through morphological, physiological and biochemical characteristics, and molecular biology identification, BWLY1PSB-1, BWLY2X-4 and BWLY3X-11 were identified as Staphylococcus pateuri, Bacillus methylotrophicus and Bacillus sp., respectively; The nematicidal activity of the fermentation supernatant of each strain could reach the maximum after 24 h treatment of nematodes, and the optimal fermentation medium was LB medium, and the nematicidal activity of the supernatant reached relatively high when cultured for five days. The research results on the growth promoting characteristics of thee strains indicated that BWLY1PSB-1 had the ability to produce iron carriers and IAA, while BWLY2X-4 and BWLY3X-11 had the ability to produce proteases, cellulases, iron carriers, and IAA. The preliminary screening of excellent strains that can effectively control plant root-knot nematodes and have rowth promoting characteristics would enrich the microbial resources for the biological control of plant root-knot nematodes.

Meloidogyne enterolobii  /  biological control  /  Staphylococcus sp.  /  Bacillus sp.  /  growth-promoting characteristics
Meng CHEN, Jiawei SHANG, Haibo LONG, Jun WANG, Lijia GUO, You ZHOU, Changcong LIANG, Yang YANG, Junsheng HUANG, Laying YANG. Screening of Biocontrol Bacteria Against Second-stage Juveniles of Meloidogyne enterolobii and Evaluation of the Growth-promoting Characteristics[J]. Chinese Journal of Tropical Crops, 2024 , 45 (10) : 2183 -2191 . DOI: 10.3969/j.issn.1000-2561.2024.10.019
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.10.019
  • Receive Date：2023-12-12
  • Online Date：2026-06-25
  • Published：2024-10-25
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History
  • Received：2023-12-12
  • Revised：2024-04-06
Funding
Affiliations
    1.College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430007, China
    2.Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences / National Key Laboratory for Tropical Crop Breeding / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / National Collection of Microbial Resource for Fertilizer (Hainan) / Collection of Tropical Agricultural Microbial Resource in Hainan Province, Haikou, Hainan 571101, China
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https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2024.10.019
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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