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Understanding the expression regulation mechanisms of enzymes related to starch synthesis in cassava can significantly advance the molecular breeding for high yield and high starch content. Prior research has uncovered that the auxin-responsive gene, MeSAUR1, upregulates the expression of MeAGPS1a, a gene encoding the small subunit of the key starch-synthesizing enzyme, AGPase. Yeast two-hybrid screening identified a Yippee protein family member, MeYippee1, as a candidate interacting protein with MeSAUR1. In this study, the coding region of the MeYippee1 gene from cassava variety SC8 was cloned. The gene was 321 bp in the coding region, lacking introns, encoding a protein of 106 amino acids, with four predicted phosphorylation sites. Expression analysis in different tissues and organs revealed that MeYippee1 was most highly expressed in axillary buds, followed by fibrous roots, storage roots, and mature leaves, while expression levels were lower in stems and petioles, and the lowest in apical buds and young leaves. Expression analysis during the different developmental stages of the storage root showed that MeYippee1 expression was primarily during the formation phase of the storage roots. Further point-to-point yeast two-hybrid verification indicated an interactive relationship between MeYippee1 and MeSAUR1 proteins. The results would provide new insights into the molecular mechanism underlying the positive regulation of MeAGPS1a expression by MeSAUR1.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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