MinD is involved in the fine regulation of plastid division and plays a key role in maintaining plastid morphology. Previous laboratory research found that the MeMinD protein is involved in the division of cassava plastids, but the related proteins that work in conjunction with MeMinD to regulate the division of cassava plastids have not been clearly identified. This study constructed the pGBKT7-MeMinD vector and, through yeast two-hybrid library screening, a total of 8 candidate interacting proteins of MeMinD were obtained; after point-to-point verification using the yeast two-hybrid system, it was found that the nicotinate phosphoribosyltransferase 2 (MeNAPRT2) interacts with MeMinD. The results of this study would provide new information for further analysis of the mechanism of MeMinD's participation in the regulation of cassava plastid splitting.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |