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Mango is a typical respiration leap fruit, with vigorous postharvest metabolism and very sensitive to ethylene.CTR1 is a negative regulator of the ethylene signaling pathway and plays a central role in the ethylene signaling pathway. In order to study the possible role of mango CTR1 expression pattern in mango postharvest storage, a CTR gene (MiCTR1) was screened from the Tainung No.1) mango transcriptome database, and its encoded gene was used using biological methods. The protein was sequenced and analyzed, and the expression pattern of MiCTR1 gene during mango ripening and under 1-MCP treatment was analyzed. The results showed that the length of the open reading frame of MiCTR1 was 1551 bp, encoding 516 amino acids, The molecular formula of the predicted protein was C2503H3932N712O797S26, the total atomic number was 7970, and the weight of the protein was 57.58 kDa, the theoretical isoelectric point (pI) was 5.72, the fat coefficient was 70.78, and the overall average hydrophilicity was -0.596, with 111 phosphorylation sites, and the phosphorylation modification of serine was the main one, supplemented by threonine, had no transmembrane structure and no signal peptide. Subcellular prediction analysis showed that it was located in the nucleus. Protein domain prediction MiCTR1 protein contained a conserved PB1 domain located at amino acid sequence 189-285. Phylogenetic analysis showed that MiCTR1 was closely related to Prunus dulcis, Prunus persica, Prunus avium, Prunus armeniaca, Prunus mume. The results of fluorescence quantitative PCR showed that the relative expression of MiCTR1 increased during the ripening process of mango, and 1-MCP down-regulated its expression. In this study, the MiCTR1 gene was cloned in mango and its biological analysis was analyzed, and its expression pattern during postharvest storage was analyzed, which would provide a basis for the molecular mechanism of mango ripening.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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