The fruit of rambutan has a delicate and juicy taste, but it is not resistant to storage. It is prone to browning 2-3 days after harvest, which limits the market circulation of rambutan. Therefore, green, safe and convenient post-harvest preservation technology is very important for the development of the rambutan industry. The purpose of the study was to explore the effects of exogenous sodium nitroprusside (SNP) treatment on the browning of postharvest rambutan peel and its active oxygen metabolism, and to provide new ideas for the browning of postharvest rambutan. The Baoyan 7 rambutan was soaked with 200 μmol/L SNP, and the control was treated with distilled water. The weight loss rate, browning index and antioxidant enzyme activity of rambutan during storage were measured. The results showed that SNP treatment could effectively inhibit the increase of browning index of postharvest rambutan peel and maintain the peel color. Compared with the control group, the activity of reactive oxygen species (ROS) scavenging enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX)] was significantly increased in the peel of rambutan treated with SNP, which improved the scavenging ability of reactive oxygen species radicals in the peel. The activities of polyphenol oxidase (PPO) and peroxidase (POD) were lower than those of the control, which effectively inhibited the occurrence of enzymatic browning. In addition, compared with the control group, SNP treatment can also significantly inhibit the increase of membrane lipid degradation-related enzyme activity (lipoxygenase, LOX) and maintain the structural integrity of pericarp cells. At the same time, the expression levels of browning-related genes NlWRKY23 and NlWRKY57 was significantly up-regulated. This shows that SNP treatment can enhance the antioxidant capacity of the peel of rambutan, reduce the oxidative damage of reactive oxygen species (ROS), and delay the occurrence of peel browning. The results of this study can provide theoretical basis and practical basis for the application of SNP in postharvest rambutan.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |