0, 60 and 120 kg/hm2 MgO treatments (denoted by Mg0, Mg60, and Mg120, respectively) on a long-term wax gourd (Tiezhu No.2) magnesium (Mg) fertilizer gradient positioning experiment was conducted in 2022 and 2023 to investigate the physiological mechanism of Mg to improve the storage resistance of wax gourd fruits by regulating pectin metabolism by measuring indicators such as fruit hardness, weight loss rate, Mg content and morphology, pectin content and morphology, and pectin metabolic enzyme activity during fruit storage. Results showed that wax gourd yield increased by 8.5% to 16.9% after Mg fertilization, with the Mg60 treatment showing the greatest increase. Compared with the Mg0 treatment, the flesh hardness of the Mg60 treatment increased by an average of 12.9%, 11.7%, and 18.9% in the two years of 0, 30, and 60 d storage, while the Mg120 treatment showed a significant difference from the Mg0 only after 30 days of storage in 2022. Suitable Mg fertilization delayed the rise of the fruit storage weight loss rate. The fruit storage weight loss rate of Mg60 after 60 d was 34.4% and 35.1% lower than that of Mg0 and Mg120 treatment. Magnesium fertilization significantly increased fruit Mg content. Alcohol-soluble (MgEth), water-soluble (MgWat), and salt-soluble Mg (MgNaCl) were the main forms of Mg in the fruit. The content gradually increased with the prolongation of storage time. During fruit storage, the total pectin content gradually decreaseg. However, appropriate Mg fertilization (Mg60) delayed the decline in water-soluble (WSP), ion-bound (CSP), and covalently bound pectin (SSP) content, thereby maintaining the total pectin content. Suitable Mg fertilization (Mg60) inhibited the activity of polygalacturonase (PG) and β-galactosidase (β-Gal), thereby slowing down the degradation process of pectin. Concurrently, it increased pectin methylesterase (PME) activity and the content of free Mg2+ (MgEth and MgWat), enabling negatively charged pectin acid to bind with Mg2+, thereby increasing CSP and SSP. This slowed the rate of hardness decline and weight loss caused by pectin degradation during fruit storage. Comprehensively, suitable Mg (60 kg/hm2) fertilization can improve the storage resistance of wax gourd by regulating pectin metabolism, which is conducive to optimizing the Mg of wax gourd, improving the storage quality, and promoting the sustainable development of the industry.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |