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Morphological and Molecular Identification of Colletotrichum kahawae Causing Anthracnose of Coffea liberica and Fungicide Selection for the Disease Management
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Xuejun LI1, Yuan REN1, 3, Hongmei CHEN1, Peng QU1, Huabo DU1, Pengbo HE2, **, Wanzhong TAN1, 4, **
Chinese Journal of Tropical Crops | 2024, 45(6) : 1273 - 1281
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Chinese Journal of Tropical Crops | 2024, 45(6): 1273-1281
• Plant Protection & Bio-safety •
Morphological and Molecular Identification of Colletotrichum kahawae Causing Anthracnose of Coffea liberica and Fungicide Selection for the Disease Management
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Xuejun LI1, Yuan REN1, 3, Hongmei CHEN1, Peng QU1, Huabo DU1, Pengbo HE2, **, Wanzhong TAN1, 4, **
Affiliations
  • 1.College of Tropic Crop, Yunnan Agricultural University, Pu’er, Yunnan 655099, China
  • 2.College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650100, China
  • 3.Yunnan Yanshan Craneberry Agriculture Co., Ltd., Wenshan, Yunan 663100, China
  • 4.West Yunnan University, Lincang, Yunnan 677000, China
Published: 2024-06-25 doi: 10.3969/j.issn.1000-2561.2024.06.019
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The present work was conducted to identify the pathogen causing anthracnose of Liberica coffee (Coffea liberica) plants and to screen fungicides against the disease pathogen. The leaves of C. liberica with anthracnose symptoms were sampled from fields for fungal isolation and purification. Four pure fungal isolates were obtained and only KFTJ01 was verified pathogenic to the coffee leaves by tests following Koch’s Postulates. The rDNA-ITS sequence (554 bp), beta-tubulin gene (tub2) and calmodulin gene (CAL) were successfully amplified via PCR from genomic DNA of KFTJ01 and sequenced. Blastn analysis of the the three DNA sequences showed that KFTJ01 was identical to the strain WZ-135 (99.62%, MN856281), CREADC-ER2212 (100%, MT409131) and YMTJ4 (100%, MK569149) of Colletotrichum kahawae. KFTJ01 was also clustered with C. kahawae WZ-135 strain on the same end-branch of the phylogenic tree (bootstrap confidence is 98%). Therefore, the pathogen of Liberica coffee was identified as C. kahawae which is a quarantine species and has never been recorded in China. Fungicide selection experiments showed that among the 10 chemicals, methionine-acetazolyl and methyl thiobacillam almost completely (100%) suppressed the colony growth of KFTJ01. The median effective concentration calculated from the functions was 0.0607 mg/mL and 0.0809 mg/mL, respectively, indicating that both the chemicals were very effective against the anthracnose pathogen. The results are important references for the field diagnosis of the coffee plant anthracnose and for controlling the disease epidemics.

Coffea liberica  /  anthracnose  /  Colletotrichum kahawae  /  multigene sequence analysis  /  fungicide
Xuejun LI, Yuan REN, Hongmei CHEN, Peng QU, Huabo DU, Pengbo HE, Wanzhong TAN. Morphological and Molecular Identification of Colletotrichum kahawae Causing Anthracnose of Coffea liberica and Fungicide Selection for the Disease Management[J]. Chinese Journal of Tropical Crops, 2024 , 45 (6) : 1273 -1281 . DOI: 10.3969/j.issn.1000-2561.2024.06.019
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Year 2024 volume 45 Issue 6
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.06.019
  • Receive Date：2023-03-30
  • Online Date：2026-06-24
  • Published：2024-06-25
Article Data
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History
  • Received：2023-03-30
  • Revised：2023-08-03
Funding
Affiliations
    1.College of Tropic Crop, Yunnan Agricultural University, Pu’er, Yunnan 655099, China
    2.College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan 650100, China
    3.Yunnan Yanshan Craneberry Agriculture Co., Ltd., Wenshan, Yunan 663100, China
    4.West Yunnan University, Lincang, Yunnan 677000, China
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https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2024.06.019
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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