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Bombax ceiba is a deciduous tree belonging to the Genus Bombax in the Malvaceae family. It holds significant importance as an ornamental plant of economic, medicinal and edible value in southern China. It is the city flower of Guangzhou and Panzhihua. The traditional propagation methods for B. ceiba are seed sowing and grafting. However, the seeds are difficult to preserve and easily deteriorate, leading to decreased germination rates. Grafting results in even lower survival rates. Therefore, this experiment aimed to study the tissue culture techniques for the rapid propagation of B. ceiba. In this study, B. ceiba seeds were used as the experimental materials, and different disinfection methods were tested to determine the most suitable method for seed disinfection, aiming to obtain sterile seedlings. Using the sterile seedlings as the explants, various basic culture media, combinations of shoot-inducing hormones, induction and proliferation media for apical and adventitious buds, as well as rooting media, were tested and optimized. Additionally, seedling acclimatization experiments were conducted to explore the optimal basic culture medium, hormone combination, induction and proliferation medium, rooting medium, and seedling acclimatization methods. The results showed that the optimal basic culture medium for B. ceiba tissue culture was MS medium supplemented with 30 g/L sucrose and 7.5 g/L agar. The best disinfection method for B. ceiba seeds was 75% ethanol treatment for 1 min, followed by five rinses with sterile water, 4-hour sterilization with a 50% H2O2 solution prepared from 30% H2O2, and another five rinses with sterile water. The optimal plant growth regulators for inducing differentiation of apical buds into young shoots were a combination of 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA). The best induction and proliferation medium was MS medium supplemented with 0.5 mg/L 6-BA, 0.5 mg/L indole-3-butyric acid (IBA), 30 g/L sucrose, and 7.5 g/L agar. The optimal rooting medium was half-strength MS medium supplemented with 0.5 mg/L IBA, 30 g/L sucrose, 7.5 g/L agar, and 0.2 g/L activated charcoal. The recommended substrate for seedling acclimatization was a mixture of sand, peat soil, and vermiculite in a ratio of 1∶1∶1. Through the optimization of seed disinfection methods, culture media selection, hormone combinations, and seedling acclimatization experiments, an efficient tissue culture system for B. ceiba was established. This would provide technical support for the in vitro preservation of B. ceiba seedlings, propagation of superior individuals, healthy seedling breeding, and the industrialized production of seedlings, and lay a foundation for the genetic transformation system of B. ceiba.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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