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The variation of volatile aroma compounds and squalene-related enzyme activities of the tissue cultivation of Pandanus amaryllifolius Roxb. leaves (PAL) treated with different concentrations of Zn and La was studied. The volatile aroma compounds were analyzed by GC-MS, and the key enzyme activities of squalene metabolism were detected and analyzed. The results showed that there were differences in the number and content of volatile compounds in the tissue cultivation of PAL treated with different concentrations of trace elements. Compared with the untreated groud, 4 mg/L Zn treatment and 1 mg/L, 2 mg/L, 4 mg/L La treatment had more volatile compounds. Zn and La treatment increased the content of alcohols and decreased the content of olefins. Zn and La treatment significantly reduced the content of squalene. The treatment of 2 mg/L Zn increased the content of β-sitosterol, stigmasterol, ethyl palmitate, 3-methyl-2-(5H)-furanone, 2,3-dihydro-3,5-dihydroxy-6-methyl-4(h)-pyran-4-one, and decreased the content of squalene and phytol. The content of phytol in the leaves of 8 mg/L La treatment was the highest. The activities of squalene metabolism-related enzymes were changed in the process of tissue culture. Lanosterol synthase (LSS) activity and squalene epoxidase (SE) activity were stronger in the mixture, and squalene synthase (SQS) activity was stronger in adventitious roots. Different concentrations of trace elements had a certain effect on the activity of squalene-related enzymes. The content of squalene was positively correlated with LSS enzyme activity, SE enzyme activity and SQS enzyme activity in the PAL treated with different concentrations of Zn. The content of squalene in the tissue cultivation PAL treated with La was negatively correlated with LSS enzyme activity and SQS enzyme activity, and positively correlated with SE enzyme activity. The results would enrich the relevant theoretical research on the regulation of trace elements on the volatile aroma compounds of PAL, and provided theoretical basis for improving the aroma quality of PAL.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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