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AT-hook nuclear localized proteins (AHLs) are small DNA-binding protein motifs that play an important role in plant growth and development, organ construction, stress and hormone signaling response. In this study, MeAHL17 was cloned from cassava cultivar SC8. Bioinformatics methods were used to analyze MeAHL17 promoter, protein physicochemical properties, prediction of conserved functional domain and sequence comparison. The results showed that the open reading frame length of MeAHL17 gene was 939 bp, encoding a protein with 312 amino acids, its theoretical isoelectric point was 6.89, its molecular formula was C1420H2215N415O451S11, and its molecular weight was 32.669 38 kDa. The total number of positively charged amino acid residues (Lys+Arg) was 23, the total number of negatively charged amino acid residues (Asp+Glu) was 24, the fat coefficient was 57.47, the total average hydrophilic coefficient (GRAVY) was -0.491 and the instability coefficient was 58.06. The protein contains no signal peptide, is located in the cell membrane, has no transmembrane structure, contains 5 glycosylation sites and 45 phosphorylation sites, and is an unstable hydrophilic acidic protein MeAHL17 contained AT-hook conserved core sequences and PPC domain conserved core sequences, which was consistent with the typical structural characteristics of AT-hook family. Through subcellular localization and transcriptional activity analysis experiments, MeAHL17 was proved to be a nucleus localized transcription factor with transcriptional activity. Tissue expression pattern analysis showed that MeAHL17 was mainly expressed in somatic embryo, fibrous root and callus. Different hormone treatments showed that MeAHL17 was induced by ethylene (ACC), methyl jasmonate (JA) and growth hormone (IAA), which suggested that MeAHL17 might be involved in ethylene, methyl jasmonate and growth hormone signaling pathway. Abiotic stress treatment showed that MeAHL17 was responsive to drought and salt stress. This study preliminarily identified the important role of MeAHL17 in growth and development, hormone signaling and stress, and provided theoretical basis and reference for further study of its function.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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